ID A0A3P8TTI1_AMPPE Unreviewed; 733 AA.
AC A0A3P8TTI1;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=procollagen-lysine 5-dioxygenase {ECO:0000256|ARBA:ARBA00012264};
DE EC=1.14.11.4 {ECO:0000256|ARBA:ARBA00012264};
OS Amphiprion percula (Orange clownfish) (Lutjanus percula).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Pomacentridae; Amphiprion.
OX NCBI_TaxID=161767 {ECO:0000313|Ensembl:ENSAPEP00000028374.1, ECO:0000313|Proteomes:UP000265080};
RN [1] {ECO:0000313|Ensembl:ENSAPEP00000028374.1, ECO:0000313|Proteomes:UP000265080}
RP NUCLEOTIDE SEQUENCE.
RA Lehmann R.;
RT "Finding Nemo's genes: A chromosome-scale reference assembly of the genome
RT of the orange clownfish Amphiprion percula.";
RL Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSAPEP00000028374.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-oxoglutarate + L-lysyl-[collagen] + O2 = (5R)-5-hydroxy-L-
CC lysyl-[collagen] + CO2 + succinate; Xref=Rhea:RHEA:16569, Rhea:RHEA-
CC COMP:12751, Rhea:RHEA-COMP:12752, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:16810, ChEBI:CHEBI:29969,
CC ChEBI:CHEBI:30031, ChEBI:CHEBI:133442; EC=1.14.11.4;
CC Evidence={ECO:0000256|ARBA:ARBA00024166};
CC -!- COFACTOR:
CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC Evidence={ECO:0000256|ARBA:ARBA00001954};
CC -!- COFACTOR:
CC Name=L-ascorbate; Xref=ChEBI:CHEBI:38290;
CC Evidence={ECO:0000256|ARBA:ARBA00001961};
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000256|ARBA:ARBA00004367}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004367}; Lumenal side
CC {ECO:0000256|ARBA:ARBA00004367}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR AlphaFoldDB; A0A3P8TTI1; -.
DR STRING; 161767.ENSAPEP00000028374; -.
DR Ensembl; ENSAPET00000029123.1; ENSAPEP00000028374.1; ENSAPEG00000020103.1.
DR GeneTree; ENSGT01030000234558; -.
DR OMA; ETMEDCG; -.
DR Proteomes; UP000265080; Chromosome 23.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0031418; F:L-ascorbic acid binding; IEA:UniProtKB-KW.
DR GO; GO:0008475; F:procollagen-lysine 5-dioxygenase activity; IEA:UniProtKB-EC.
DR GO; GO:0008045; P:motor neuron axon guidance; IEA:Ensembl.
DR GO; GO:0001755; P:neural crest cell migration; IEA:Ensembl.
DR CDD; cd23002; GT_LH3; 1.
DR Gene3D; 2.60.120.620; q2cbj1_9rhob like domain; 1.
DR InterPro; IPR044861; IPNS-like_FE2OG_OXY.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR InterPro; IPR006620; Pro_4_hyd_alph.
DR InterPro; IPR001006; Procol_lys_dOase.
DR PANTHER; PTHR10730:SF7; MULTIFUNCTIONAL PROCOLLAGEN LYSINE HYDROXYLASE AND GLYCOSYLTRANSFERASE LH3; 1.
DR PANTHER; PTHR10730; PROCOLLAGEN-LYSINE,2-OXOGLUTARATE 5-DIOXYGENASE/GLYCOSYLTRANSFERASE 25 FAMILY MEMBER; 1.
DR Pfam; PF03171; 2OG-FeII_Oxy; 1.
DR SMART; SM00702; P4Hc; 1.
DR SUPFAM; SSF53448; Nucleotide-diphospho-sugar transferases; 1.
DR PROSITE; PS51471; FE2OG_OXY; 1.
DR PROSITE; PS01325; LYS_HYDROXYLASE; 1.
PE 4: Predicted;
KW Dioxygenase {ECO:0000256|ARBA:ARBA00022964};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000265080};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP};
KW Vitamin C {ECO:0000256|ARBA:ARBA00022896}.
FT SIGNAL 1..21
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 22..733
FT /note="procollagen-lysine 5-dioxygenase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5018069747"
FT DOMAIN 640..733
FT /note="Fe2OG dioxygenase"
FT /evidence="ECO:0000259|PROSITE:PS51471"
SQ SEQUENCE 733 AA; 84894 MW; 11C7376EE7F61BC8 CRC64;
MSSWRILCLA ALAFFQSSTS AEKRRLSPEN LLVITAATEE TDGFQRFMRT AREFNYTVKV
LGLGEDWKGG DVARTVGGGQ KVRWLKKELL KHSDNKDLVV MFVDSYDVIF ASGPEELLSK
FSRLGHRVVF SAEGFCWPDQ RLASKYPEVH SGKRYLNSGG FIGFAADLSA IVQQWKYKDN
DDDQLFYTRI FLDKTQRTKF NITLDHRSRI FQNLNGAVDE VVLKFERAKV RARNVAYDTL
PVIIHGNGPT KLQLNYLGNY VPTAWTYENG CGICDDDLLF FTDVPDEELP LVYVAVFIEH
ATPFMEEFLD RLTTLNYPTD RIRLFIHNNV VYHERHIQKF WERYRALFPD ALLVGPEEDL
KEDKARTMAV EACKKDPECD YYFSIDSDVA LTNPDTLRIL IEENKSVIAP MLSKHGKLWS
NFWGALSPEG YYSRSEDYIE IVQGKRIGLW NVPYITQAYL IKGSMLRSKL SQVSLYVDDG
MDPDMVFCRS IRDQGVFMFV SNRDEFGRLV ASANFNTSRL HPDMWQIFDN PMDWKEKYVH
ENYSKIFEDE KSFVEQPCPD VYWFPAFSEK MCDHLVETME DYGEWSGGSH KDERLAGGYE
NVPTVDIHMN QIGFEKEWLK FLKEYIAPVT EKLYPGYYPK AQAIMNFVVR YRPDEQPSLR
PHHDSSTFTI NIALNNKGVD YEGGGCKFLR YDCKVESPRK GWSFMHPGRL THYHEGLPTT
RGTRYIMVSF VDP
//
