ID A0A3P8UKC7_CYNSE Unreviewed; 986 AA.
AC A0A3P8UKC7;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN Name=AREL1 {ECO:0000313|Ensembl:ENSCSEP00000001096.1};
OS Cynoglossus semilaevis (Tongue sole).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Carangaria; Pleuronectiformes; Pleuronectoidei; Cynoglossidae;
OC Cynoglossinae; Cynoglossus.
OX NCBI_TaxID=244447 {ECO:0000313|Ensembl:ENSCSEP00000001096.1, ECO:0000313|Proteomes:UP000265120};
RN [1] {ECO:0000313|Ensembl:ENSCSEP00000001096.1, ECO:0000313|Proteomes:UP000265120}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=24487278;
RA Chen S., Zhang G., Shao C., Huang Q., Liu G., Zhang P., Song W., An N.,
RA Chalopin D., Volff J.N., Hong Y., Li Q., Sha Z., Zhou H., Xie M., Yu Q.,
RA Liu Y., Xiang H., Wang N., Wu K., Yang C., Zhou Q., Liao X., Yang L.,
RA Hu Q., Zhang J., Meng L., Jin L., Tian Y., Lian J., Yang J., Miao G.,
RA Liu S., Liang Z., Yan F., Li Y., Sun B., Zhang H., Zhang J., Zhu Y., Du M.,
RA Zhao Y., Schartl M., Tang Q., Wang J.;
RT "Whole-genome sequence of a flatfish provides insights into ZW sex
RT chromosome evolution and adaptation to a benthic lifestyle.";
RL Nat. Genet. 46:253-260(2014).
RN [2] {ECO:0000313|Ensembl:ENSCSEP00000001096.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|PROSITE-ProRule:PRU00104}.
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DR AlphaFoldDB; A0A3P8UKC7; -.
DR Ensembl; ENSCSET00000001124.1; ENSCSEP00000001096.1; ENSCSEG00000000729.1.
DR GeneTree; ENSGT00940000156723; -.
DR OMA; NLCIFGP; -.
DR Proteomes; UP000265120; Chromosome 1.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR CDD; cd00078; HECTc; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR017868; Filamin/ABP280_repeat-like.
DR InterPro; IPR001298; Filamin/ABP280_rpt.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR PANTHER; PTHR11254:SF340; APOPTOSIS-RESISTANT E3 UBIQUITIN PROTEIN LIGASE 1; 1.
DR PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR Pfam; PF00630; Filamin; 1.
DR Pfam; PF00632; HECT; 1.
DR SMART; SM00119; HECTc; 1.
DR SMART; SM00557; IG_FLMN; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR PROSITE; PS50194; FILAMIN_REPEAT; 1.
DR PROSITE; PS50237; HECT; 1.
PE 4: Predicted;
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000265120};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT TRANSMEM 177..196
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 202..221
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT REPEAT 250..356
FT /note="Filamin"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00087"
FT DOMAIN 680..986
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 521..543
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 524..543
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 986 AA; 113728 MW; 4DA4F925A7509D07 CRC64;
MDRRFFLTFF FCTVSWIFFW EVRWKKGKES QIKEWLQGHS LSQYRHLFED VQTLEELSLS
VLSRLEEVVK EQQRWRQIAE AHIQLLRDFA LQEWLCSQNL EHYYHTLKTL GCANLDELSE
FDSELQLSLA AWGYYYEDYI KLSTGIKILK TTRGSRDQDY EIRLAHSLAE RRLNEKWSIA
GALIFGCTVA LCFLIRDLMF YVIGGITVSI IAFVFTIKFL CELAARVVSF LQNEDPGRRG
DRSIYDYVRG NYLDPRSCKV SWDWKEPQEV GQTMSFRVQL FYKNGQPFPA HRPVGLRVHI
THIELALDIP VTQEVLQEPE SNVVKVTFTV RKAGRYEVAV KLGGLNVAYS PYYKIFQPGT
VVPSKTKIAY HFSTLVLVNG QQHTLQIEPR DEYGNPTSNS TSLTDEANYS VRVHSLGTVD
DDNLEEYCSK SVSLNKPQCQ VLLRLTLRKS GCFRARISYK KQLLSNGEFD VIVLSENEKT
CVERNVSTPG ISIYFEAYLY SSGNYSSSTW QFPASSLLAP QRRPSMGEEE DEHDSPVEGQ
PEKVKKPKKV YCYISPKQLS VKEFYLKIIP WRLFTFRVCP GTKFTYYGPD PVHKYQTLVV
DDGIQPPVEL SCKDRNIMAA TFIRFLHKNI GGSETFQDKV VFFQRELRHI HVKKPRTKTC
LKITRHSILD SSLKATRNFS VSDWSKNFEV VFQDEEALDW GGPRREWFEL VCKTLFDTSN
QLFNRFSDNN QGLVHPNPDR PPHLRLKMYE FAGRVVGKCL YESALGGAYK QLVRARFTRS
FLAQIIGLRM NYKYFETDDQ EFYKTKVCFI LNNDVSEMDL VFAEEKYSKS GQLEKVVELI
SGGAQIAVTN ENKMHYLNLL AQYRLASQVR DEVEHFLKGL NELVPENLLA IFDENELELL
MCGTGDINVQ DFKAHAVIVG GSWHFREKVM KWFWAVVSSF TQEELARLLQ FTTGSSQLPP
GGFNTLSPSF QIIAAPTHST LPTAHT
//
