ID A0A3P8V305_CYNSE Unreviewed; 1534 AA.
AC A0A3P8V305;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 24-JAN-2024, entry version 18.
DE SubName: Full=UDP-glucose glycoprotein glucosyltransferase 1 {ECO:0000313|Ensembl:ENSCSEP00000008494.1};
OS Cynoglossus semilaevis (Tongue sole).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Carangaria; Pleuronectiformes; Pleuronectoidei; Cynoglossidae;
OC Cynoglossinae; Cynoglossus.
OX NCBI_TaxID=244447 {ECO:0000313|Ensembl:ENSCSEP00000008494.1, ECO:0000313|Proteomes:UP000265120};
RN [1] {ECO:0000313|Ensembl:ENSCSEP00000008494.1, ECO:0000313|Proteomes:UP000265120}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=24487278;
RA Chen S., Zhang G., Shao C., Huang Q., Liu G., Zhang P., Song W., An N.,
RA Chalopin D., Volff J.N., Hong Y., Li Q., Sha Z., Zhou H., Xie M., Yu Q.,
RA Liu Y., Xiang H., Wang N., Wu K., Yang C., Zhou Q., Liao X., Yang L.,
RA Hu Q., Zhang J., Meng L., Jin L., Tian Y., Lian J., Yang J., Miao G.,
RA Liu S., Liang Z., Yan F., Li Y., Sun B., Zhang H., Zhang J., Zhu Y., Du M.,
RA Zhao Y., Schartl M., Tang Q., Wang J.;
RT "Whole-genome sequence of a flatfish provides insights into ZW sex
RT chromosome evolution and adaptation to a benthic lifestyle.";
RL Nat. Genet. 46:253-260(2014).
RN [2] {ECO:0000313|Ensembl:ENSCSEP00000008494.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=N(4)-(alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-
CC (1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-
CC alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-
CC GlcNAc-(1->4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan
CC mannose isomer 9A1,2,3B1,2,3) + UDP-alpha-D-glucose = H(+) + N(4)-
CC (alpha-D-Glc-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-
CC Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-
CC (1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-
CC beta-D-GlcNAc-(1->4)-beta-D-GlcNAc)-L-asparaginyl-[protein] + UDP;
CC Xref=Rhea:RHEA:61304, Rhea:RHEA-COMP:14356, Rhea:RHEA-COMP:14357,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58223, ChEBI:CHEBI:58885,
CC ChEBI:CHEBI:59080, ChEBI:CHEBI:139493;
CC Evidence={ECO:0000256|ARBA:ARBA00034426};
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
CC -!- PATHWAY: Protein modification; protein glycosylation.
CC {ECO:0000256|ARBA:ARBA00004922}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum lumen
CC {ECO:0000256|ARBA:ARBA00004319}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 8 family.
CC {ECO:0000256|ARBA:ARBA00006351}.
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DR Ensembl; ENSCSET00000008583.1; ENSCSEP00000008494.1; ENSCSEG00000005390.1.
DR GeneTree; ENSGT00390000004600; -.
DR UniPathway; UPA00378; -.
DR Proteomes; UP000265120; Chromosome 1.
DR GO; GO:0005788; C:endoplasmic reticulum lumen; IEA:UniProtKB-SubCell.
DR GO; GO:0003980; F:UDP-glucose:glycoprotein glucosyltransferase activity; IEA:InterPro.
DR GO; GO:0006486; P:protein glycosylation; IEA:UniProtKB-UniPathway.
DR CDD; cd06432; GT8_HUGT1_C_like; 1.
DR InterPro; IPR040497; Glyco_transf_24.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR InterPro; IPR009448; UDP-g_GGtrans.
DR InterPro; IPR040693; UGGT_TRXL_1.
DR InterPro; IPR040694; UGGT_TRXL_2.
DR InterPro; IPR040692; UGGT_TRXL_3.
DR InterPro; IPR040525; UGGT_TRXL_4.
DR PANTHER; PTHR11226; UDP-GLUCOSE GLYCOPROTEIN:GLUCOSYLTRANSFERASE; 1.
DR PANTHER; PTHR11226:SF3; UDP-GLUCOSE:GLYCOPROTEIN GLUCOSYLTRANSFERASE 1; 1.
DR Pfam; PF18404; Glyco_transf_24; 1.
DR Pfam; PF18400; Thioredoxin_12; 1.
DR Pfam; PF18401; Thioredoxin_13; 1.
DR Pfam; PF18402; Thioredoxin_14; 1.
DR Pfam; PF18403; Thioredoxin_15; 1.
DR Pfam; PF06427; UDP-g_GGTase; 1.
DR SUPFAM; SSF53448; Nucleotide-diphospho-sugar transferases; 1.
PE 3: Inferred from homology;
KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Reference proteome {ECO:0000313|Proteomes:UP000265120};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT SIGNAL 1..32
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 33..1534
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5018336363"
FT DOMAIN 50..233
FT /note="UGGT thioredoxin-like"
FT /evidence="ECO:0000259|Pfam:PF18400"
FT DOMAIN 310..439
FT /note="UGGT thioredoxin-like"
FT /evidence="ECO:0000259|Pfam:PF18401"
FT DOMAIN 449..697
FT /note="UGGT thioredoxin-like"
FT /evidence="ECO:0000259|Pfam:PF18402"
FT DOMAIN 723..945
FT /note="UDP-glucose:glycoprotein glucosyltransferase
FT thioredoxin-like"
FT /evidence="ECO:0000259|Pfam:PF18403"
FT DOMAIN 1247..1514
FT /note="Glucosyltransferase 24 catalytic"
FT /evidence="ECO:0000259|Pfam:PF18404"
SQ SEQUENCE 1534 AA; 173890 MW; 8C00B26B2CC82E7B CRC64;
MSPLLTIFSP GIGVRMWLLW ALLFSLVSAS SAGADSKAVT TTLTTKWADT PLLLEASEFL
AEESQEKFWD FVESNQNIEG EHDDTDQAYY ELIVKKASAL LSSVQLNMLK FALSLRAYSA
TVHSFQQCDY IPGDTFIASN EPPPSGCSAF FNVHGEKTCD ADGLDALLKM APERPKPYLF
KGDHKYSGSN PDAPVVILYA EFGKPHFRDL HEVVASKARE GLVTYVLRHY LAVSRKVYLS
GYGVELAIKS QEYKAKDDTQ VQGTEVNATV IGENDPVDEV QGFLFGKLKT IYPELKEQLK
ELRKHLVEST NEMAPLKVWQ MQDLSFQTAA RILAAPAVEA LNVMKDLSQN FPTKARSITK
TVVNSEIRKE IGDNQKFFKG TLGLQPGDSA LFINGLHIDM DTQDIFSVFE ILRSEARVME
GLRSLLIDTP FIHDILKLNV QPSDSDYAVD IRNSAINWIN NLEADHRYSS WPSNVQELLR
PTFPGVIRQI RKNFHNLVMI LDPSQESASE LLSVAEMFYA NNIPLRIGLV FVVSDEDDID
GMQDPGVALV RAYNYISDEV NSQSAFEAVI SMFNRVPVGA KLSVGDVVKV LEKRFPYVEV
SSVLGADSSY DSNRKEGRAY YEQTGVGPLP VVMYNGIPYQ REQLDSDELE TVTMQKILET
TSFYQRAVYL GELVSDQDVV DFIMNQPNVV PRINPRVLST SRTYLDLSDT NNYFVDDYAR
FSTLDTKEKS TAVANSMNYM TKKDDGYIRP VTFWVVGDFD RPSGRKLLYD AIRHMKTSNN
IRLAMINNPS TDPSAETTRV ARAIWAAMQT QSANNAKNFI TKMAKEETAA TLQEDTDIGD
FAVGGMDVVL FKSAYDSPQF DVLRSHATYC RDVLKLKKGQ RAVISNGRII GPLEDEEVFN
QDDFLLLESI ILKTSGERIK NKVQHFEIEE DRASDLVMKV DALLSSQPKG EARVEYDFAD
DRYSAVKIRP KEGDMYFDVV AVVDPVTRDA QKLAPLLTVL KQLVNVNMRV FMNCQSKLSE
IPLKSFYRYV LEPEVMFQSD GSYSPGPLAK FLDMPQSPLF TLNLNTPESW MVESVHTRYD
LDNIYLEEVD NIVAAEYELE HLLLEGHCFD VSSGQPPRGL QFTLGTASEP VIVDTIVMAN
LGYFQLKANP GAWILKLRKG RSDEIYKIYS HDGTDSPADS DDVIVVLNNF KSRIIKVKVQ
KKADKLNEEL LSDGTEENDT GFWESLTRGF TGGGKVEEVK QDKDDVINIF SVASGHLYER
FLRIMMLSVR KHTNTPVKFW FLKNYLSPTF KEFIPHMAKE YGFQYELVQY KWPRWLHQQT
EKQRIIWGYK ILFLDVLFPL AVDKILFVDA DQIVRTDLKE LRDLDLEGAP YGYTPFCESR
REMDGYRFWK SGYWASHLAG RKYHISALYV VDLKKFRKIA AGDRLRGQYQ GLSQDPNSLS
NLDQDLPNNM IHQVPIKSLP QDWLWCETWC DNSSKKNAKT IDLCNNPMTK EPKLQAAVRI
VAEWTDYDQE IKRLQTRVQE GATLRPQEQQ GTGS
//
