ID A0A3P8VU76_CYNSE Unreviewed; 1218 AA.
AC A0A3P8VU76;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 24-JAN-2024, entry version 19.
DE RecName: Full=isoleucine--tRNA ligase {ECO:0000256|ARBA:ARBA00013165};
DE EC=6.1.1.5 {ECO:0000256|ARBA:ARBA00013165};
DE AltName: Full=Isoleucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00032665};
OS Cynoglossus semilaevis (Tongue sole).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Carangaria; Pleuronectiformes; Pleuronectoidei; Cynoglossidae;
OC Cynoglossinae; Cynoglossus.
OX NCBI_TaxID=244447 {ECO:0000313|Ensembl:ENSCSEP00000015950.1, ECO:0000313|Proteomes:UP000265120};
RN [1] {ECO:0000313|Ensembl:ENSCSEP00000015950.1, ECO:0000313|Proteomes:UP000265120}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=24487278;
RA Chen S., Zhang G., Shao C., Huang Q., Liu G., Zhang P., Song W., An N.,
RA Chalopin D., Volff J.N., Hong Y., Li Q., Sha Z., Zhou H., Xie M., Yu Q.,
RA Liu Y., Xiang H., Wang N., Wu K., Yang C., Zhou Q., Liao X., Yang L.,
RA Hu Q., Zhang J., Meng L., Jin L., Tian Y., Lian J., Yang J., Miao G.,
RA Liu S., Liang Z., Yan F., Li Y., Sun B., Zhang H., Zhang J., Zhu Y., Du M.,
RA Zhao Y., Schartl M., Tang Q., Wang J.;
RT "Whole-genome sequence of a flatfish provides insights into ZW sex
RT chromosome evolution and adaptation to a benthic lifestyle.";
RL Nat. Genet. 46:253-260(2014).
RN [2] {ECO:0000313|Ensembl:ENSCSEP00000015950.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-
CC isoleucyl-tRNA(Ile); Xref=Rhea:RHEA:11060, Rhea:RHEA-COMP:9666,
CC Rhea:RHEA-COMP:9695, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58045, ChEBI:CHEBI:78442, ChEBI:CHEBI:78528,
CC ChEBI:CHEBI:456215; EC=6.1.1.5;
CC Evidence={ECO:0000256|ARBA:ARBA00000114};
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594, ECO:0000256|RuleBase:RU363035}.
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DR AlphaFoldDB; A0A3P8VU76; -.
DR Ensembl; ENSCSET00000016150.1; ENSCSEP00000015950.1; ENSCSEG00000010191.1.
DR GeneTree; ENSGT00550000074921; -.
DR Proteomes; UP000265120; Chromosome 11.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd07961; Anticodon_Ia_Ile_ABEc; 1.
DR CDD; cd00818; IleRS_core; 1.
DR Gene3D; 3.40.50.620; HUPs; 2.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR033709; Anticodon_Ile_ABEc.
DR InterPro; IPR002301; Ile-tRNA-ligase.
DR InterPro; IPR023586; Ile-tRNA-ligase_type2.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR NCBIfam; TIGR00392; ileS; 1.
DR PANTHER; PTHR42780:SF1; ISOLEUCINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR PANTHER; PTHR42780; SOLEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF19302; DUF5915; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR PRINTS; PR00984; TRNASYNTHILE.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|RuleBase:RU363035};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363035};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363035};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363035};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW ECO:0000256|RuleBase:RU363035};
KW Reference proteome {ECO:0000313|Proteomes:UP000265120}.
FT DOMAIN 94..592
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 647..804
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
SQ SEQUENCE 1218 AA; 139346 MW; C971BC0A23CCCCD2 CRC64;
MVEPVPESIN FPNEEEKVLK FWQDKDCFQE CLKQSKNRPR YTFYDGPPFA TGLPHYGHIL
AGTIKDIVTR FAHQSGYHVE RRFGWDCHGL PVTSVKRMGR WIDFKNDYKT LYPWFMETVW
WVFKQLYDKG LVYRGVKVMP FSTACNTPLS NFESHQNYKD VQDPSVIVNF PLLESEDISL
IAWTTTPWTL PSNLALCVNP EFIYVKVKVY ILMEARLGAL FKSESEYTLL DKFPGKMLKG
KKYKPLFPYF AKCAEKGAFQ VVTDNYVKEE EGTGVVHQAP YFGADDYRVC TEYSIIQKDQ
APICPVDASG CFTSEVTDFA GEYVKDADKN IIKWLKEKGR LVNASTYKHS YPFCWRSDTP
LIYKAVPSWF VRVEHMVDKL LDNNGKCYWV PEFVREKRFG NWLRDARDWA ISRNRYWGTP
IPMWVSDDFE EVVCVGSTAE LEELTGTKVT DLHRENIDSL TIPSRCGKGT LRRVTEVFDC
WFESGSMPYA QVHYPFENRK EFEDTFPADF IAEGIDQTRG WFYTLLVLST ALFGKPPFKN
VIVNGLVLAS DGQKMSKRKK NYPDPGLIVQ NYGADALRLY LINSPVVRAE NLRFKEEGVR
DMLKDVFLPW YNAYRFLVQN VQRLQKEEGT VFLYNENTAK PSDNIMDKWI QSFTQSLIQF
FRAEMDAYRL YTVVPRLVKF VDMLTNWYVR TNRRRLKGET GTQDCLWALE TLFSVLFSMC
RLMAPFTPFV TEMMYQNLRH LIDPVSFDEK DTSSIHYLML PQVRESLIDK QIENAVSQMQ
SIIELGRVIR DRKTLPVKYP LKEVIVIHQD PEALKNIQSL QKYILEELNV RQLTLSTDKD
KYGIRLRAEP DHMVLGKRLK GAFKAVTASI KELTNEHLET FQKTGSIIVD GHELHEEDLR
LMYTFDQDSN CAAQYEAHSD EQVLVLLDVT PDQSMVDEGV AREVINRIQK LRKKAHLVPS
DEITVYYRCQ PEGEYLHSVI QAHTDFILAT TKAPLLPSPV PSKAVVIIEE KTQLKGSDLE
FTIVKGSSAS PVCLDGPACS YLNLRVKINN KEQEGVVLLE NPKGDNRLTV DKLKVVCSSI
FGSNNTQLRL FNGETGEDLN ALSGKTLSVT SDSSSPGSSS SSDGVLCPYV NLLLCNAQPA
ECLTGEVGTL LLFNPVGQNG LDYSGLLSET AKVFGLRNRR TKLYLDQDLT QELPSDFCVK
SLDTKTLFVK VLPTTAEA
//
