ID A0A3P9BU84_9CICH Unreviewed; 1350 AA.
AC A0A3P9BU84;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE SubName: Full=NPC1-like intracellular cholesterol transporter 1 {ECO:0000313|Ensembl:ENSMZEP00005013540.1};
GN Name=NPC1L1 {ECO:0000313|Ensembl:ENSMZEP00005013540.1};
OS Maylandia zebra (zebra mbuna).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Cichlomorphae; Cichliformes; Cichlidae; African cichlids;
OC Pseudocrenilabrinae; Haplochromini; Maylandia; Maylandia zebra complex.
OX NCBI_TaxID=106582 {ECO:0000313|Ensembl:ENSMZEP00005013540.1, ECO:0000313|Proteomes:UP000265160};
RN [1] {ECO:0000313|Ensembl:ENSMZEP00005013540.1, ECO:0000313|Proteomes:UP000265160}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=25186727; DOI=10.1038/nature13726;
RA Brawand D., Wagner C.E., Li Y.I., Malinsky M., Keller I., Fan S.,
RA Simakov O., Ng A.Y., Lim Z.W., Bezault E., Turner-Maier J., Johnson J.,
RA Alcazar R., Noh H.J., Russell P., Aken B., Alfoldi J., Amemiya C.,
RA Azzouzi N., Baroiller J.F., Barloy-Hubler F., Berlin A., Bloomquist R.,
RA Carleton K.L., Conte M.A., D'Cotta H., Eshel O., Gaffney L., Galibert F.,
RA Gante H.F., Gnerre S., Greuter L., Guyon R., Haddad N.S., Haerty W.,
RA Harris R.M., Hofmann H.A., Hourlier T., Hulata G., Jaffe D.B., Lara M.,
RA Lee A.P., MacCallum I., Mwaiko S., Nikaido M., Nishihara H.,
RA Ozouf-Costaz C., Penman D.J., Przybylski D., Rakotomanga M., Renn S.C.P.,
RA Ribeiro F.J., Ron M., Salzburger W., Sanchez-Pulido L., Santos M.E.,
RA Searle S., Sharpe T., Swofford R., Tan F.J., Williams L., Young S., Yin S.,
RA Okada N., Kocher T.D., Miska E.A., Lander E.S., Venkatesh B., Fernald R.D.,
RA Meyer A., Ponting C.P., Streelman J.T., Lindblad-Toh K., Seehausen O.,
RA Di Palma F.;
RT "The genomic substrate for adaptive radiation in African cichlid fish.";
RL Nature 513:375-381(2014).
RN [2] {ECO:0000313|Ensembl:ENSMZEP00005013540.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=cholesterol(in) = cholesterol(out); Xref=Rhea:RHEA:39747,
CC ChEBI:CHEBI:16113; Evidence={ECO:0000256|ARBA:ARBA00034049};
CC -!- SIMILARITY: Belongs to the patched family.
CC {ECO:0000256|ARBA:ARBA00005585}.
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DR RefSeq; XP_004544258.1; XM_004544201.1.
DR STRING; 106582.ENSMZEP00005013540; -.
DR Ensembl; ENSMZET00005013999.1; ENSMZEP00005013540.1; ENSMZEG00005010178.1.
DR GeneID; 101465425; -.
DR KEGG; mze:101465425; -.
DR CTD; 29881; -.
DR GeneTree; ENSGT00940000159904; -.
DR OrthoDB; 2786103at2759; -.
DR Proteomes; UP000265160; LG12.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0005319; F:lipid transporter activity; IEA:InterPro.
DR GO; GO:0030301; P:cholesterol transport; IEA:UniProt.
DR Gene3D; 1.20.1640.10; Multidrug efflux transporter AcrB transmembrane domain; 2.
DR InterPro; IPR004765; NPC1-like.
DR InterPro; IPR032190; NPC1_N.
DR InterPro; IPR003392; Ptc/Disp.
DR InterPro; IPR000731; SSD.
DR NCBIfam; TIGR00917; 2A060601; 1.
DR PANTHER; PTHR45727; NPC INTRACELLULAR CHOLESTEROL TRANSPORTER 1; 1.
DR PANTHER; PTHR45727:SF3; NPC1-LIKE INTRACELLULAR CHOLESTEROL TRANSPORTER 1; 1.
DR Pfam; PF16414; NPC1_N; 1.
DR Pfam; PF02460; Patched; 1.
DR Pfam; PF12349; Sterol-sensing; 1.
DR SUPFAM; SSF82866; Multidrug efflux transporter AcrB transmembrane domain; 2.
DR PROSITE; PS50156; SSD; 1.
PE 3: Inferred from homology;
KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000265160};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Transport {ECO:0000256|ARBA:ARBA00022448}.
FT SIGNAL 1..19
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 20..1350
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5017956901"
FT TRANSMEM 282..308
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 366..387
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 650..671
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 683..707
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 713..736
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 756..783
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 789..814
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 861..882
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1131..1152
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1159..1180
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1186..1209
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1230..1249
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1261..1284
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 649..814
FT /note="SSD"
FT /evidence="ECO:0000259|PROSITE:PS50156"
FT REGION 1324..1350
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1326..1341
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1350 AA; 150480 MW; AA31B6D91BB0146E CRC64;
MMRLAALVIF INIVSLSEAQ HEPGYCVFYD ECGRNPLLNN TLIDPIVPCL NYTRAKLVTG
DHYKILKQVC PMFDQGESST YACCTIKQLG SLEKSLTLSK AVLVRCPSCA YNFAHLHCIN
TCSPNQSQTV KVTRVMNVTE QNRTREAVLG YQAFIGKTFA DTSFQSCKNV RIPATIGGYA
IATMCGRYGS KLCTPQRWYD FQGDSSNGLA PLDIDFKIIQ EGDTTGLPEG VIPYNGVALM
CNETTPTGGD VCSCQDCQES CPSVLPPSPV AGPFTLLGTN GYLVISIILL ILLILSFVLY
LSVSWWVASQ KNKKKGIHRG KGKDKDSDKV TEKIIHPSEV TCAETNSLAA QAYLSSQFRI
WGTIMASYPL TVLLLSLIVV AVLSVGLKDI KLTTDPVELW SAPNSRARQE KEFHDTYFDP
FYRTNQVILT APGRKSHIYD SLLFGPQNFS GIMSKELIIE LLELQTRIQN IQFWSDDLNR
TASLKDVCFA PLNPNNPSLT DCAVNSLPQY FQNSLDHINA KVNMTELGVT KEVDWRDHLI
YCLGSPLSFK DITDLGMSCM ADYGAPVFPF LAVGGYEDDA FSSAEAFILT FSLNNYARSD
PKFKVAMQWE KEFLKIVQEY QKDPKNSFTF AYMAERSLED EINRTTAEDI PIFMISYAVI
FVYIAVALGE HSSWKRLLVD SKFLVGLGGI LVVACSVLAS MGFYSWIGIP SSLVILQVVP
FLVLAVGADN IFIFVLEYQR DVRRPHETRE EQIGRVLGNV APSMLLCSLS ESVCFFLGAL
STMPAVKSFA LYAALAVLMD FALQMTAFVA LLSLDARRQD NNRCELLCCI KVSKQRPKKP
NKGFLMPFMK KYYAPVLLHR YTRIIVIVVF IFMFCGALFL MMNVKVGLDQ ELAMPQDSYM
LTYFQYLNKY FEVGVPVYFV TKKGFNFTSV EGMNAVCSSV GCDQFSLTQK IQYATNYPER
SYVAIPANSW VDDFIDWLNP QSKCCRLYTN GPNVGHFCPA NESGLICAKR CLARPDNDTV
RPTVAQFNRY LPDFLTNRPD LQCSKGGLGA YDKAVVRDES GEIIASRFMA YHTPLTNSQE
FTAALKMARE LADKITQGMR LVPGTSPDFE VFPYTITYVF YEQYLTIVNE GLFNISLCLL
PTFVVCCLLL GLDLRSGLLN LLTIVMIVVD TVGVMTLWSI DYNAVALINL VTAVGISVEF
VSHMTRSFAL STKPTRVERA IEATAKMGSA VFAGVAMTNL PGIIVLAFAK AQLIQIFFFR
LNLVITLLGM AHGLIFLPVV LSYFGPGVNK AVLLQFQQEK EKDREKAETN SHMRQVYDNI
SYEGNEIKQD PYSNTVDESG SKTVGKTDRL
//
