ID A0A3Q0RMR8_AMPCI Unreviewed; 1125 AA.
AC A0A3Q0RMR8;
DT 10-APR-2019, integrated into UniProtKB/TrEMBL.
DT 10-APR-2019, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE SubName: Full=Solute carrier family 12 member 2 {ECO:0000313|Ensembl:ENSACIP00000011891.1};
OS Amphilophus citrinellus (Midas cichlid) (Cichlasoma citrinellum).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Cichlomorphae; Cichliformes; Cichlidae; New World cichlids;
OC Cichlasomatinae; Heroini; Amphilophus.
OX NCBI_TaxID=61819 {ECO:0000313|Ensembl:ENSACIP00000011891.1, ECO:0000313|Proteomes:UP000261340};
RN [1] {ECO:0000313|Ensembl:ENSACIP00000011891.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 chloride(out) + K(+)(out) + Na(+)(out) = 2 chloride(in) +
CC K(+)(in) + Na(+)(in); Xref=Rhea:RHEA:72395, ChEBI:CHEBI:17996,
CC ChEBI:CHEBI:29101, ChEBI:CHEBI:29103;
CC Evidence={ECO:0000256|ARBA:ARBA00034631};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:72396;
CC Evidence={ECO:0000256|ARBA:ARBA00034631};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the SLC12A transporter family.
CC {ECO:0000256|ARBA:ARBA00010593}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR AlphaFoldDB; A0A3Q0RMR8; -.
DR STRING; 61819.ENSACIP00000011891; -.
DR Ensembl; ENSACIT00000012224.1; ENSACIP00000011891.1; ENSACIG00000009236.1.
DR GeneTree; ENSGT00940000155742; -.
DR OMA; DTISFEM; -.
DR Proteomes; UP000261340; Unplaced.
DR GO; GO:0016323; C:basolateral plasma membrane; IEA:Ensembl.
DR GO; GO:0015377; F:chloride:monoatomic cation symporter activity; IEA:Ensembl.
DR GO; GO:0042802; F:identical protein binding; IEA:Ensembl.
DR GO; GO:0042472; P:inner ear morphogenesis; IEA:Ensembl.
DR GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR GO; GO:0048798; P:swim bladder inflation; IEA:Ensembl.
DR Gene3D; 1.20.1740.10; Amino acid/polyamine transporter I; 1.
DR InterPro; IPR004841; AA-permease/SLC12A_dom.
DR InterPro; IPR013612; AA_permease_N.
DR InterPro; IPR002444; NKCC1.
DR InterPro; IPR018491; SLC12_C.
DR InterPro; IPR002443; SLC12A1/SLC12A2.
DR InterPro; IPR004842; SLC12A_fam.
DR NCBIfam; TIGR00930; 2a30; 1.
DR PANTHER; PTHR11827:SF58; SOLUTE CARRIER FAMILY 12 MEMBER 2; 1.
DR PANTHER; PTHR11827; SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS; 1.
DR Pfam; PF00324; AA_permease; 1.
DR Pfam; PF08403; AA_permease_N; 1.
DR Pfam; PF03522; SLC12; 1.
DR PRINTS; PR01207; NAKCLTRNSPRT.
DR PRINTS; PR01208; NAKCLTRSPRT1.
PE 3: Inferred from homology;
KW Ion transport {ECO:0000256|ARBA:ARBA00023201};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Sodium {ECO:0000256|ARBA:ARBA00023053};
KW Sodium transport {ECO:0000256|ARBA:ARBA00023201};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00023201}.
FT TRANSMEM 250..279
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 299..320
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 341..360
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 366..383
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 417..434
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 454..476
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 525..549
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 585..602
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 614..632
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 652..682
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 134..187
FT /note="Amino acid permease N-terminal"
FT /evidence="ECO:0000259|Pfam:PF08403"
FT DOMAIN 222..725
FT /note="Amino acid permease/ SLC12A"
FT /evidence="ECO:0000259|Pfam:PF00324"
FT DOMAIN 734..1125
FT /note="SLC12A transporter C-terminal"
FT /evidence="ECO:0000259|Pfam:PF03522"
FT REGION 1..81
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 93..130
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 890..916
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..16
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 110..130
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 899..916
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1125 AA; 123073 MW; 0870A6C65304F7B8 CRC64;
MSAPPSALSA PAQNSAAEND FLAPDAGQRP PGPTPSQSRF QVDLVAEAAG AADDKTSSSD
ATAAASEAPS TDPAVGGEEA KGRFRVVNFA DPTGAGSMAS PDAAPTEGMQ NGDTVMSETS
LHSSTGGQHH YHYDTHTNTY YLRTFGHNTI DAVPNIDFYR QTAAPLGEKL IRPTLSELHD
ELDKEPFEDG FANGDELTPA EEAAAKEAAE SKGVVKFGWI KGVLVRCMLN IWGVMLFIRM
SWIVGQAGIV LTCVIISMAT VVTTITGLST SAIATNGFVR GGGAYYLISR SLGPEFGGSI
GLIFAFANAV AVAMYVVGFA ETVVEILVNN DAVMTDQLND IRIIGTITII ILLGISVAGM
EWEAKAQIIL LVVLVSAIFN YFIGSFIPIK SKEPAGFFGY DASLMWENMG PDFREETFFS
VFAIFFPAAT GILAGTNISG DLADPQMAIP KGTLLAILIT GIVYLGVAVS AGSCILRDAS
GNMNDTISSQ FMENCSTAAC KFGYDFSTCK SQNACRYGLH NDFQVMSLVS GFGPVITAGI
FSATLSSALA SLVSAPKVFQ ALCKDNIYPG LQMFAKGYGK NNEPLRGYIL TFGIALGFIL
IAKLNTIAPI ISNFFLASYA LINFSVFHAS LANSPGWRPS FKYYSMWVSL AGAILCCGVM
FVINWAAALL TNIIVMALYI YVSHKKPDVN WGSSTQALTY HQALTHTLHL SGVEDHIKNF
RPQCLVMTGY PNSRPALLDL VHSFTKNVGL MICGHIRMGY RRPNFKELAT DQTRYQRWLL
KNETKAFYMP VLAEDLRQGC QYLLQAAGLG RLKPNTLVLG FKNDWRDGDM MNVETYISMI
HDAFDFQFGA VILRLKEGLD VSHIQGQDEL LSSQEKSSGM KDVIVSIDTS KDSDADSSKP
SSKATNKKSP TVPLNVSDQR LLEASQQFQR KQGKGTVDVW WLFDDGGLTL LIPYLLTNKM
RWKECKIRVF IGGKINRIDH DRRAMATLLS KFRIDFSDIT VLGDINTKPK KEHMAAFEEM
IEPYRLKEDD MEQEAAERLK NSEPWRITDN ELELYRAKTY RQLRLNELLK EHSSTANLIV
MSLPLARKGA VSSALYMAWL EALSKDLPPI LLVRGNHQSV LTFYS
//
