ID A0A3Q0S7C2_AMPCI Unreviewed; 546 AA.
AC A0A3Q0S7C2;
DT 10-APR-2019, integrated into UniProtKB/TrEMBL.
DT 10-APR-2019, sequence version 1.
DT 24-JAN-2024, entry version 23.
DE RecName: Full=glutaminase {ECO:0000256|ARBA:ARBA00012918};
DE EC=3.5.1.2 {ECO:0000256|ARBA:ARBA00012918};
OS Amphilophus citrinellus (Midas cichlid) (Cichlasoma citrinellum).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Cichlomorphae; Cichliformes; Cichlidae; New World cichlids;
OC Cichlasomatinae; Heroini; Amphilophus.
OX NCBI_TaxID=61819 {ECO:0000313|Ensembl:ENSACIP00000018971.1, ECO:0000313|Proteomes:UP000261340};
RN [1] {ECO:0000313|Ensembl:ENSACIP00000018971.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + L-glutamine = L-glutamate + NH4(+);
CC Xref=Rhea:RHEA:15889, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC ChEBI:CHEBI:29985, ChEBI:CHEBI:58359; EC=3.5.1.2;
CC Evidence={ECO:0000256|ARBA:ARBA00001062};
CC -!- SIMILARITY: Belongs to the glutaminase family.
CC {ECO:0000256|ARBA:ARBA00011076}.
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DR AlphaFoldDB; A0A3Q0S7C2; -.
DR STRING; 61819.ENSACIP00000018971; -.
DR Ensembl; ENSACIT00000019482.1; ENSACIP00000018971.1; ENSACIG00000014748.1.
DR GeneTree; ENSGT00390000010463; -.
DR OMA; EYQQDWK; -.
DR Proteomes; UP000261340; Unplaced.
DR GO; GO:0004359; F:glutaminase activity; IEA:UniProtKB-EC.
DR GO; GO:0006541; P:glutamine metabolic process; IEA:InterPro.
DR Gene3D; 1.10.238.210; -; 1.
DR Gene3D; 1.25.40.20; Ankyrin repeat-containing domain; 1.
DR Gene3D; 3.40.710.10; DD-peptidase/beta-lactamase superfamily; 1.
DR HAMAP; MF_00313; Glutaminase; 1.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR012338; Beta-lactam/transpept-like.
DR InterPro; IPR015868; Glutaminase.
DR InterPro; IPR041541; Glutaminase_EF-hand.
DR NCBIfam; TIGR03814; Gln_ase; 1.
DR PANTHER; PTHR12544; GLUTAMINASE; 1.
DR PANTHER; PTHR12544:SF33; GLUTAMINASE LIVER ISOFORM, MITOCHONDRIAL; 1.
DR Pfam; PF12796; Ank_2; 1.
DR Pfam; PF17959; EF-hand_14; 1.
DR Pfam; PF04960; Glutaminase; 1.
DR SMART; SM00248; ANK; 2.
DR SUPFAM; SSF48403; Ankyrin repeat; 1.
DR SUPFAM; SSF56601; beta-lactamase/transpeptidase-like; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 1.
PE 3: Inferred from homology;
KW ANK repeat {ECO:0000256|ARBA:ARBA00023043, ECO:0000256|PROSITE-
KW ProRule:PRU00023}; Coiled coil {ECO:0000256|SAM:Coils};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Repeat {ECO:0000256|ARBA:ARBA00022737}.
FT DOMAIN 25..108
FT /note="Glutaminase EF-hand"
FT /evidence="ECO:0000259|Pfam:PF17959"
FT REPEAT 462..484
FT /note="ANK"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00023"
FT COILED 509..536
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 546 AA; 61199 MW; 2DD798521E6A1F18 CRC64;
MIVFLFSSVS SSLMHMHSKP SCSGVENMLF YTITEGKEQV PISYFTAALK KTGLHPSDPR
LKDCMDNIRQ AVKESVGEVM MDRDLFHRCV GGNIVLLIQA FRKKFIIPEF DVFVQKINEI
YEKVQNQSDG KVADYIPQLA KFSPELWGVS LCTVDGQRHS VGHTKQPFCL QSCVKPLQYA
IAVHESGTEK VHRYVGMEPS GLKFNVLSLD DEDKPHNPMV NPGAIVISSL IKVDRYVLAE
SARTVYHSLF SKRQFQSEKE TGDRNFAIGY YMKEKKCFPP GADMIDALDF YFQLCSIEVT
CESGSIMAAT LANGGICPIT GERILSAEAV RNTLSLMHSC GMYDFSGQMA FHVGLPAKSG
VSGAILLVIP NVMGVMCWSP PLDRVGNSVR GIHFCQELVS VFNFHNYDNL SHFVKKQDPR
RQDGEDRNRS VFNLLFAAYS GDVSALRRFA LSSMDMDLKD HDSRTALHVA AAEGHMDVVK
FLTETCKADP FVEDRWGNLP VNDAMQFGHD EVVKVLKDYQ QECRQQEKQH SEAVHSKKLD
TIEGMV
//
