ID   A0A3Q1FXF0_9TELE        Unreviewed;       633 AA.
AC   A0A3Q1FXF0;
DT   10-APR-2019, integrated into UniProtKB/TrEMBL.
DT   10-APR-2019, sequence version 1.
DT   27-MAR-2024, entry version 24.
DE   SubName: Full=AlkB homolog 8, tRNA methyltransferase {ECO:0000313|Ensembl:ENSAPOP00000021169.1};
GN   Name=ALKBH8 {ECO:0000313|Ensembl:ENSAPOP00000021169.1};
OS   Acanthochromis polyacanthus (spiny chromis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC   Ovalentaria; Pomacentridae; Acanthochromis.
OX   NCBI_TaxID=80966 {ECO:0000313|Ensembl:ENSAPOP00000021169.1, ECO:0000313|Proteomes:UP000257200};
RN   [1] {ECO:0000313|Ensembl:ENSAPOP00000021169.1}
RP   IDENTIFICATION.
RG   Ensembl;
RL   Submitted (NOV-2023) to UniProtKB.
CC   -!- COFACTOR:
CC       Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC         Evidence={ECO:0000256|ARBA:ARBA00001954};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC       Nucleus {ECO:0000256|ARBA:ARBA00004123}.
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DR   AlphaFoldDB; A0A3Q1FXF0; -.
DR   Ensembl; ENSAPOT00000016969.1; ENSAPOP00000021169.1; ENSAPOG00000000864.1.
DR   GeneTree; ENSGT00940000158563; -.
DR   Proteomes; UP000257200; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0008757; F:S-adenosylmethionine-dependent methyltransferase activity; IEA:InterPro.
DR   GO; GO:0008175; F:tRNA methyltransferase activity; IEA:UniProt.
DR   GO; GO:0043412; P:macromolecule modification; IEA:UniProt.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProt.
DR   CDD; cd02440; AdoMet_MTases; 1.
DR   Gene3D; 2.60.120.590; Alpha-ketoglutarate-dependent dioxygenase AlkB-like; 1.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR027450; AlkB-like.
DR   InterPro; IPR037151; AlkB-like_sf.
DR   InterPro; IPR013216; Methyltransf_11.
DR   InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR13069:SF21; ALKYLATED DNA REPAIR PROTEIN ALKB HOMOLOG 8; 1.
DR   PANTHER; PTHR13069; UNCHARACTERIZED; 1.
DR   Pfam; PF13532; 2OG-FeII_Oxy_2; 1.
DR   Pfam; PF08241; Methyltransf_11; 1.
DR   SUPFAM; SSF51197; Clavaminate synthase-like; 1.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR   PROSITE; PS51471; FE2OG_OXY; 1.
PE   4: Predicted;
KW   Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW   Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW   Reference proteome {ECO:0000313|Proteomes:UP000257200};
KW   RNA-binding {ECO:0000256|ARBA:ARBA00022884};
KW   Transferase {ECO:0000256|ARBA:ARBA00022603};
KW   Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT   DOMAIN          116..236
FT                   /note="Fe2OG dioxygenase"
FT                   /evidence="ECO:0000259|PROSITE:PS51471"
FT   REGION          200..220
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          428..472
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          500..583
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        500..528
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        529..551
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        564..583
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   633 AA;  69693 MW;  550C6D2CEBD0146E CRC64;
     MIQCGDNSVT LYLSYVESVT HEEQTSVLLP EGLVLVEDFV SPEEEAVLLS AIDWSSTNDD
     ITAQKALKHR RVKHYGFEFR YDNNNVDKDK PLPAGLPEEC LPVLERCVSS EHISMMPDQL
     TVNQYESGQG IPPHVDTHSA FEDTILSLSL GAKTVMDFRH PDGRVVAVVL PGRSLLVMKG
     ESRYLWTHGI TPRKFDMVPA CDPQSSARSR PDLGTQSSLT LSRRGTRTSF TFRKIRHEPC
     RCAFPSACDS RGAPSTPSPA LPPSPPTLPC CRADAARLEE EYVHRVYDAI ASHFSSTRHS
     PWPRVCHFLA SLPPGSVLAD VGCGNGKYLG VNPDVIAVGC DRSGALIQIC AERGFHAFVS
     DALSVPLRTA SCDACISIAV IHHFSTQERR LAAVRELVRL LKPGGRALIY VWAFEQEYNK
     QKSKYLKESN KEHQGNHSPT SEDSQEPHGK SSIHISSHLE DSCRPDDSMT DVSRVTDGKL
     SVHTNRTAFN TQDLLVPWHL KEGKKRDDEE SGDSSKKGKS KEKNKKTSHN SCSASSLNPK
     TDPSPGSGSR LDTSDAAHIP DCKPSPSGNT TQTSSSTVKS ELESSPAPVF HRYYHVFQQG
     ELEQLCAQVN GVKVQSSYHD QGNWCVILQK DGL
//