ID   A0A3Q1JLF9_ANATE        Unreviewed;       571 AA.
AC   A0A3Q1JLF9;
DT   10-APR-2019, integrated into UniProtKB/TrEMBL.
DT   02-JUN-2021, sequence version 2.
DT   27-MAR-2024, entry version 21.
DE   RecName: Full=Perforin 1.2 {ECO:0008006|Google:ProtNLM};
OS   Anabas testudineus (Climbing perch) (Anthias testudineus).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC   Anabantaria; Anabantiformes; Anabantoidei; Anabantidae; Anabas.
OX   NCBI_TaxID=64144 {ECO:0000313|Ensembl:ENSATEP00000033935.2, ECO:0000313|Proteomes:UP000265040};
RN   [1] {ECO:0000313|Ensembl:ENSATEP00000033935.2}
RP   IDENTIFICATION.
RG   Ensembl;
RL   Submitted (NOV-2023) to UniProtKB.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC   -!- SIMILARITY: Belongs to the complement C6/C7/C8/C9 family.
CC       {ECO:0000256|ARBA:ARBA00009214}.
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DR   AlphaFoldDB; A0A3Q1JLF9; -.
DR   Ensembl; ENSATET00000034427.2; ENSATEP00000033935.2; ENSATEG00000023259.2.
DR   GeneTree; ENSGT00530000063725; -.
DR   Proteomes; UP000265040; Unplaced.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.40.150; C2 domain; 1.
DR   InterPro; IPR000008; C2_dom.
DR   InterPro; IPR035892; C2_domain_sf.
DR   InterPro; IPR020864; MACPF.
DR   InterPro; IPR020863; MACPF_CS.
DR   PANTHER; PTHR46096:SF5; PERFORIN 1.3-RELATED; 1.
DR   PANTHER; PTHR46096; PERFORIN-1; 1.
DR   Pfam; PF00168; C2; 1.
DR   Pfam; PF01823; MACPF; 1.
DR   SMART; SM00239; C2; 1.
DR   SMART; SM00457; MACPF; 1.
DR   SUPFAM; SSF49562; C2 domain (Calcium/lipid-binding domain, CaLB); 1.
DR   PROSITE; PS50004; C2; 1.
DR   PROSITE; PS00279; MACPF_1; 1.
DR   PROSITE; PS51412; MACPF_2; 1.
PE   3: Inferred from homology;
KW   Cytolysis {ECO:0000256|ARBA:ARBA00022852};
KW   Disulfide bond {ECO:0000256|ARBA:ARBA00023157};
KW   Reference proteome {ECO:0000313|Proteomes:UP000265040};
KW   Secreted {ECO:0000256|ARBA:ARBA00022525}; Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           26..571
FT                   /note="Perforin 1.2"
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5030080502"
FT   DOMAIN          29..373
FT                   /note="MACPF"
FT                   /evidence="ECO:0000259|PROSITE:PS51412"
FT   DOMAIN          390..512
FT                   /note="C2"
FT                   /evidence="ECO:0000259|PROSITE:PS50004"
SQ   SEQUENCE   571 AA;  63508 MW;  F2796DB22ED1083D CRC64;
     TREFDFNNLS RLLLSLPLCL SSTVSFIGTP QECKDAHFVP GYNLGGEGFD IVTMERKGAY
     VIDTETWNLG NGTCKMYRNS YMNREKQKVP VGVVDWRTLP KCSLKVSSTV YDSVESLVND
     STSSVSNDWK VGLSIPVHAS VTVGVGFGGS HSRDATFGMQ KSKQDRYNFF RHAVFCRFYS
     YRLATKPPLS QEFKSAVNSL PAYSRKTEEF YRNLIDTYGT HYIIKVSLGG EIKAITSVRT
     CQATMNGLSA TEISDCLSVE ASASFANSAS INAMYKHCQA KKKTLGSHQS FSSTYNERNT
     EVIGGHIDSA DILFQSQSNP SVYGSWLSSL KHTPDVVQYS IKPLHTILPR GHPASAGLKQ
     EVEKYIKKNA VLKKCSESCQ IGHRSSKRDP CDCVCNSNQN VKSNCCPAGK GLATLKVFKL
     YAQGLYGDKW TQTDGSVEVS YGDRKKRTII ISNNDNPKWP ETFEFGPISI NMKDRLKFRV
     YDEDTYWNSD LLGECSIELH EGKVSDSCML KHGTLFFSYI VECAPSLSGD QCQEYTPSPM
     SPSLAKVFYT RNGVLLGESE EQFAKSVIQS D
//