ID A0A3Q1LK90_BOVIN Unreviewed; 813 AA.
AC A0A3Q1LK90;
DT 10-APR-2019, integrated into UniProtKB/TrEMBL.
DT 10-APR-2019, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=Cysteine--tRNA ligase, cytoplasmic {ECO:0000256|ARBA:ARBA00039362};
DE EC=6.1.1.16 {ECO:0000256|ARBA:ARBA00012832};
DE AltName: Full=Cysteinyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00031499};
GN Name=CARS1 {ECO:0000313|Ensembl:ENSBTAP00000057025.1,
GN ECO:0000313|VGNC:VGNC:26773};
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913 {ECO:0000313|Ensembl:ENSBTAP00000057025.1, ECO:0000313|Proteomes:UP000009136};
RN [1] {ECO:0000313|Ensembl:ENSBTAP00000057025.1, ECO:0000313|Proteomes:UP000009136}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Hereford {ECO:0000313|Ensembl:ENSBTAP00000057025.1,
RC ECO:0000313|Proteomes:UP000009136};
RA Rosen B.D., Bickhart D.M., Koren S., Schnabel R.D., Hall R., Zimin A.,
RA Dreischer C., Schultheiss S., Schroeder S.G., Elsik C.G., Couldrey C.,
RA Liu G.E., Van Tassell C.P., Phillippy A.M., Smith T.P.L., Medrano J.F.;
RT "ARS-UCD1.2.";
RL Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSBTAP00000057025.1}
RP IDENTIFICATION.
RC STRAIN=Hereford {ECO:0000313|Ensembl:ENSBTAP00000057025.1};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Catalyzes the ATP-dependent ligation of cysteine to
CC tRNA(Cys). {ECO:0000256|ARBA:ARBA00037196}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-
CC cysteinyl-tRNA(Cys); Xref=Rhea:RHEA:17773, Rhea:RHEA-COMP:9661,
CC Rhea:RHEA-COMP:9679, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:35235, ChEBI:CHEBI:78442, ChEBI:CHEBI:78517,
CC ChEBI:CHEBI:456215; EC=6.1.1.16;
CC Evidence={ECO:0000256|ARBA:ARBA00036537};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:17774;
CC Evidence={ECO:0000256|ARBA:ARBA00036537};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947};
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DR AlphaFoldDB; A0A3Q1LK90; -.
DR Ensembl; ENSBTAT00000084728.1; ENSBTAP00000057025.1; ENSBTAG00000010777.6.
DR VEuPathDB; HostDB:ENSBTAG00000010777; -.
DR VGNC; VGNC:26773; CARS1.
DR GeneTree; ENSGT00390000006347; -.
DR OMA; FHNDMKS; -.
DR Proteomes; UP000009136; Chromosome 29.
DR Bgee; ENSBTAG00000010777; Expressed in surface of tongue and 106 other cell types or tissues.
DR ExpressionAtlas; A0A3Q1LK90; baseline and differential.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004817; F:cysteine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006423; P:cysteinyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd00672; CysRS_core; 1.
DR CDD; cd10310; GST_C_CysRS_N; 1.
DR Gene3D; 1.20.1050.130; -; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR HAMAP; MF_00041; Cys_tRNA_synth; 1.
DR InterPro; IPR015803; Cys-tRNA-ligase.
DR InterPro; IPR024909; Cys-tRNA/MSH_ligase.
DR InterPro; IPR036282; Glutathione-S-Trfase_C_sf.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR032678; tRNA-synt_1_cat_dom.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR NCBIfam; TIGR00435; cysS; 1.
DR PANTHER; PTHR10890:SF3; CYSTEINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR PANTHER; PTHR10890; CYSTEINYL-TRNA SYNTHETASE; 1.
DR Pfam; PF01406; tRNA-synt_1e; 1.
DR PRINTS; PR00983; TRNASYNTHCYS.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF47616; GST C-terminal domain-like; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
PE 1: Evidence at protein level;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Proteomics identification {ECO:0007829|PeptideAtlas:A0A3Q1LK90};
KW Reference proteome {ECO:0000313|Proteomes:UP000009136};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT DOMAIN 126..536
FT /note="tRNA synthetases class I catalytic"
FT /evidence="ECO:0000259|Pfam:PF01406"
FT REGION 740..761
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 813 AA; 90487 MW; F2C60C7C0B5F4B21 CRC64;
MAGSTTEHAP DYTAILGISD EAARLRALDA YLSTRSYLQG FALSQVDVDA FRQLSGPPAD
PQLFHVARWF RHVTAILGSP PAARPPCGLQ ATGGGRRAQP TWSPPAGSEP CQLRLYNSLT
RRKDVFVPQD GRRVTWYCCG PTVYDASHMG HARSYISFDI LRRVLRDYFK FDVFYCMNIT
DIDDKIIKRA RQNFLLERYR ETQPQAAQLL EDVRAALQPF SAKLQETVDP DKKQMLERLQ
HAVAQAAEPL EGALRAGQAG QELDGRVQAL LEEAKDVLSD WLDSMHGSEV TDNSIFSELP
KFWEAEFHKD MEALNVLPPD VLTRVSEYVP EIVDFVQKIV DNGYGYASNG SVYFDTVKFA
NTEGHSYGKL VPEAVGDQKA LHEGEGDLSV SAERLSEKRS PNDFALWKAS KPGEPSWPCP
WGKGRPGWHI ECSAMAGTLL GASMDIHGGG FDLRFPHHDN ELAQSEAYFE NDCWVRYFLH
TGHLTIAGCK MSKSLKNFIT IKDALKKHSA RQLRLAFLMH SWKDTLDYSA NTMESALQYE
RLLNEFFLNV KDLLRAPVEV AAQFEKWEDE ETELNKSFYK KKAAVHQALC DNIDTRTVLE
EMRALVGQCN LYMAARKAAR RRPHRVLLES IAHYLTHMLK IFGAIEEESS LGFPIGGAGT
SLNLESTVMP YLQVLSEFRE GVRRIAREHR VPEVLQLSDA LRDNVLPELG VRLEDHEGGS
SGCDVGAGWG LPVAAGSQEG SCWDGSEGTA PLTGSAGPDR ASWASRQPLL SERRHLGLCS
CGLTARHPDS AGTCPSGGSL CRVDRQRVSQ LVF
//
