ID A0A3Q1LSK1_BOVIN Unreviewed; 1188 AA.
AC A0A3Q1LSK1;
DT 10-APR-2019, integrated into UniProtKB/TrEMBL.
DT 10-APR-2019, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE SubName: Full=Solute carrier family 12 member 2 {ECO:0000313|Ensembl:ENSBTAP00000060229.1};
GN Name=SLC12A2 {ECO:0000313|Ensembl:ENSBTAP00000060229.1,
GN ECO:0000313|VGNC:VGNC:34665};
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913 {ECO:0000313|Ensembl:ENSBTAP00000060229.1, ECO:0000313|Proteomes:UP000009136};
RN [1] {ECO:0000313|Ensembl:ENSBTAP00000060229.1, ECO:0000313|Proteomes:UP000009136}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Hereford {ECO:0000313|Ensembl:ENSBTAP00000060229.1,
RC ECO:0000313|Proteomes:UP000009136};
RA Rosen B.D., Bickhart D.M., Koren S., Schnabel R.D., Hall R., Zimin A.,
RA Dreischer C., Schultheiss S., Schroeder S.G., Elsik C.G., Couldrey C.,
RA Liu G.E., Van Tassell C.P., Phillippy A.M., Smith T.P.L., Medrano J.F.;
RT "ARS-UCD1.2.";
RL Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSBTAP00000060229.1}
RP IDENTIFICATION.
RC STRAIN=Hereford {ECO:0000313|Ensembl:ENSBTAP00000060229.1};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 chloride(out) + K(+)(out) + Na(+)(out) = 2 chloride(in) +
CC K(+)(in) + Na(+)(in); Xref=Rhea:RHEA:72395, ChEBI:CHEBI:17996,
CC ChEBI:CHEBI:29101, ChEBI:CHEBI:29103;
CC Evidence={ECO:0000256|ARBA:ARBA00034631};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:72396;
CC Evidence={ECO:0000256|ARBA:ARBA00034631};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the SLC12A transporter family.
CC {ECO:0000256|ARBA:ARBA00010593}.
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DR RefSeq; XP_005209144.1; XM_005209087.2.
DR AlphaFoldDB; A0A3Q1LSK1; -.
DR Ensembl; ENSBTAT00000082866.1; ENSBTAP00000060229.1; ENSBTAG00000009637.6.
DR GeneID; 286845; -.
DR CTD; 6558; -.
DR VEuPathDB; HostDB:ENSBTAG00000009637; -.
DR VGNC; VGNC:34665; SLC12A2.
DR GeneTree; ENSGT00940000155742; -.
DR OrthoDB; 5490251at2759; -.
DR Proteomes; UP000009136; Chromosome 7.
DR Bgee; ENSBTAG00000009637; Expressed in prostate gland and 109 other cell types or tissues.
DR ExpressionAtlas; A0A3Q1LSK1; baseline.
DR GO; GO:0016324; C:apical plasma membrane; IEA:UniProt.
DR GO; GO:0015377; F:chloride:monoatomic cation symporter activity; IEA:InterPro.
DR GO; GO:0015079; F:potassium ion transmembrane transporter activity; IEA:UniProt.
DR GO; GO:0035725; P:sodium ion transmembrane transport; IEA:UniProt.
DR Gene3D; 1.20.1740.10; Amino acid/polyamine transporter I; 1.
DR InterPro; IPR004841; AA-permease/SLC12A_dom.
DR InterPro; IPR013612; AA_permease_N.
DR InterPro; IPR002444; NKCC1.
DR InterPro; IPR018491; SLC12_C.
DR InterPro; IPR002443; SLC12A1/SLC12A2.
DR InterPro; IPR004842; SLC12A_fam.
DR NCBIfam; TIGR00930; 2a30; 1.
DR PANTHER; PTHR11827:SF58; SOLUTE CARRIER FAMILY 12 MEMBER 2; 1.
DR PANTHER; PTHR11827; SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS; 1.
DR Pfam; PF00324; AA_permease; 1.
DR Pfam; PF08403; AA_permease_N; 1.
DR Pfam; PF03522; SLC12; 1.
DR PRINTS; PR01207; NAKCLTRNSPRT.
DR PRINTS; PR01208; NAKCLTRSPRT1.
PE 3: Inferred from homology;
KW Ion transport {ECO:0000256|ARBA:ARBA00023201};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000009136};
KW Sodium {ECO:0000256|ARBA:ARBA00023053};
KW Sodium transport {ECO:0000256|ARBA:ARBA00023201};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00023201}.
FT TRANSMEM 283..303
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 309..335
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 356..380
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 400..419
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 428..449
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 479..501
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 513..533
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 591..614
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 647..667
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 673..691
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 703..722
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 728..743
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 194..245
FT /note="Amino acid permease N-terminal"
FT /evidence="ECO:0000259|Pfam:PF08403"
FT DOMAIN 282..784
FT /note="Amino acid permease/ SLC12A"
FT /evidence="ECO:0000259|Pfam:PF00324"
FT DOMAIN 793..1188
FT /note="SLC12A transporter C-terminal"
FT /evidence="ECO:0000259|Pfam:PF03522"
FT REGION 1..126
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 139..185
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 953..973
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 161..175
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 953..969
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1188 AA; 128921 MW; 96E5F4337765A5CF CRC64;
MEPGPTAPSS GAPRLAGVGE TPPVAPLAAA GMDLPGTAVP SVPEDAAAVS RGGGGVRGEG
AAAAGDGLGR PLGPTPSQSR FQVDLVSENA GRAAAAAASA GAGGKETPAD GKASVESGQA
KASEEAKGRF RVNFVDPAAS SSADESVSDA AGIGGDGPNV SFQNGGDTVL SEGSSLHSGG
GGGGSGHHQH YYYDTHTNTY YLRTFGHNTM DAVPRIDHYR HTAAQLGEKL LRPSLAELHD
ELEKEPFEDG FANGEESTPT REAVVTYTAE SKGVVKFGWI KGVLVRCMLN IWGVMLFIRL
SWIVGQAGIG LSVIVIAMAT VVTTITGLST SAIATNGFVR GGGAYYLISR SLGPEFGGAI
GLIFAFANAV AVAMYVVGFA ETVVELLKEH SILMIDEIND IRIIGAITVV ILLGISVAGM
EWEAKAQIVL LVILLLAIAD FVIGTFIPLE SKKPKGFFGY KYEIFSENFG PDFREEETFF
SVFAIFFPAA TGILAGANIS GDLADPQSAI PKGTLLAILI TTLVYIGIAV SVGSCVVRDA
TGNVNDTIVT ELTNCTSAAC KLNFDFSSCE TNPCSYGLMN NFQVMSMVSG FAPLISAGIF
SATLSSALAS LVSAPKIFQA LCKDNIYPAF QMFAKGYGKN NEPLRGYILT FLIALGFILI
AELNVIAPII SNFFLASYAL INFSVFHASL AKSPGWRPAF KYYNMWISLL GAILCCIVMF
VINWWAALLT YVIVLGLYIY VTYKKPDVNW GSSTQALTYL NALQHSIRLS GVEDHVKNFR
PQCLVMTGAP NSRPALLHLV HDFTKNVGLM ICGHVHMGPR RQAMKEMSID QAKYQRWLIK
NKMKAFYAPV HADDLREGAQ YLMQAAGLGR MKPNTLVLGF KKDWLQADMR DVDMYINLFH
DAFDIQYGVV VIRLQEGLDI SHLQGQEELL SSQEKSPGVK DVVLSVEYSK KSDLDTSKPS
SEKSITHKES KGPAVPLNVA DQKLLEASTQ FQKKQGKNTI DVWWLFDDGG LTLLIPYLLT
TKKKWKDCKI RVFIGGKINR IDHDRRAMAT LLSKFRIDFS DIMVLGDINT KPKKENIVAF
DEMIEPYRLH EDDKEQDIAD KMKEDEPWRI TDNELELYKT KTYRQIRLNE LLKEHSNTAN
IIVMSLPVAR KGAVSSALYM AWLEALSKDL PPILLVRGNH QSVLTFYS
//
