ID A0A3Q1LUK5_BOVIN Unreviewed; 1484 AA.
AC A0A3Q1LUK5;
DT 10-APR-2019, integrated into UniProtKB/TrEMBL.
DT 10-APR-2019, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE RecName: Full=Rap guanine nucleotide exchange factor 2 {ECO:0000256|ARBA:ARBA00016709};
DE AltName: Full=Cyclic nucleotide ras GEF {ECO:0000256|ARBA:ARBA00031545};
DE AltName: Full=Neural RAP guanine nucleotide exchange protein {ECO:0000256|ARBA:ARBA00032021};
DE AltName: Full=PDZ domain-containing guanine nucleotide exchange factor 1 {ECO:0000256|ARBA:ARBA00030673};
DE AltName: Full=RA-GEF-1 {ECO:0000256|ARBA:ARBA00029925};
DE AltName: Full=Ras/Rap1-associating GEF-1 {ECO:0000256|ARBA:ARBA00031980};
GN Name=RAPGEF2 {ECO:0000313|Ensembl:ENSBTAP00000058915.1,
GN ECO:0000313|VGNC:VGNC:33723};
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913 {ECO:0000313|Ensembl:ENSBTAP00000058915.1, ECO:0000313|Proteomes:UP000009136};
RN [1] {ECO:0000313|Ensembl:ENSBTAP00000058915.1, ECO:0000313|Proteomes:UP000009136}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Hereford {ECO:0000313|Ensembl:ENSBTAP00000058915.1,
RC ECO:0000313|Proteomes:UP000009136};
RA Rosen B.D., Bickhart D.M., Koren S., Schnabel R.D., Hall R., Zimin A.,
RA Dreischer C., Schultheiss S., Schroeder S.G., Elsik C.G., Couldrey C.,
RA Liu G.E., Van Tassell C.P., Phillippy A.M., Smith T.P.L., Medrano J.F.;
RT "ARS-UCD1.2.";
RL Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSBTAP00000058915.1}
RP IDENTIFICATION.
RC STRAIN=Hereford {ECO:0000313|Ensembl:ENSBTAP00000058915.1};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- SUBCELLULAR LOCATION: Cell junction {ECO:0000256|ARBA:ARBA00004282}.
CC Cell membrane {ECO:0000256|ARBA:ARBA00004236}. Cytoplasm, perinuclear
CC region {ECO:0000256|ARBA:ARBA00004556}. Endosome
CC {ECO:0000256|ARBA:ARBA00004177}. Late endosome
CC {ECO:0000256|ARBA:ARBA00004603}.
CC -!- SIMILARITY: Belongs to the RAPGEF2 family.
CC {ECO:0000256|ARBA:ARBA00010829}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR Ensembl; ENSBTAT00000069736.1; ENSBTAP00000058915.1; ENSBTAG00000012450.6.
DR VEuPathDB; HostDB:ENSBTAG00000012450; -.
DR VGNC; VGNC:33723; RAPGEF2.
DR GeneTree; ENSGT00940000156418; -.
DR Proteomes; UP000009136; Chromosome 17.
DR Bgee; ENSBTAG00000012450; Expressed in cardiac ventricle and 107 other cell types or tissues.
DR ExpressionAtlas; A0A3Q1LUK5; baseline and differential.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-SubCell.
DR GO; GO:0005770; C:late endosome; IEA:UniProtKB-SubCell.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR CDD; cd00038; CAP_ED; 1.
DR CDD; cd00992; PDZ_signaling; 1.
DR CDD; cd01785; RA_PDZ-GEF1; 1.
DR CDD; cd00155; RasGEF; 1.
DR CDD; cd06224; REM; 1.
DR Gene3D; 2.30.42.10; -; 1.
DR Gene3D; 2.60.120.10; Jelly Rolls; 1.
DR Gene3D; 1.10.840.10; Ras guanine-nucleotide exchange factors catalytic domain; 1.
DR Gene3D; 1.20.870.10; Son of sevenless (SoS) protein Chain: S domain 1; 1.
DR InterPro; IPR000595; cNMP-bd_dom.
DR InterPro; IPR018490; cNMP-bd_dom_sf.
DR InterPro; IPR001478; PDZ.
DR InterPro; IPR036034; PDZ_sf.
DR InterPro; IPR000159; RA_dom.
DR InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR InterPro; IPR023578; Ras_GEF_dom_sf.
DR InterPro; IPR001895; RASGEF_cat_dom.
DR InterPro; IPR036964; RASGEF_cat_dom_sf.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR45161; CYTOSKELETON-ASSOCIATED PROTEIN 4; 1.
DR PANTHER; PTHR45161:SF2; RAP GUANINE NUCLEOTIDE EXCHANGE FACTOR 2; 1.
DR Pfam; PF00595; PDZ; 1.
DR Pfam; PF00788; RA; 1.
DR Pfam; PF00617; RasGEF; 1.
DR Pfam; PF00618; RasGEF_N; 1.
DR SMART; SM00100; cNMP; 1.
DR SMART; SM00228; PDZ; 1.
DR SMART; SM00314; RA; 1.
DR SMART; SM00147; RasGEF; 1.
DR SMART; SM00229; RasGEFN; 1.
DR SUPFAM; SSF51206; cAMP-binding domain-like; 1.
DR SUPFAM; SSF50156; PDZ domain-like; 1.
DR SUPFAM; SSF48366; Ras GEF; 1.
DR SUPFAM; SSF54236; Ubiquitin-like; 1.
DR PROSITE; PS50042; CNMP_BINDING_3; 1.
DR PROSITE; PS50106; PDZ; 1.
DR PROSITE; PS50200; RA; 1.
DR PROSITE; PS50009; RASGEF_CAT; 1.
DR PROSITE; PS50212; RASGEF_NTER; 1.
PE 3: Inferred from homology;
KW Cell junction {ECO:0000256|ARBA:ARBA00022949};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Differentiation {ECO:0000256|ARBA:ARBA00022782};
KW Endosome {ECO:0000256|ARBA:ARBA00022753};
KW Guanine-nucleotide releasing factor {ECO:0000256|ARBA:ARBA00022658,
KW ECO:0000256|PROSITE-ProRule:PRU00168};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Neurogenesis {ECO:0000256|ARBA:ARBA00022902};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000009136};
KW Ubl conjugation {ECO:0000256|ARBA:ARBA00022843}.
FT DOMAIN 133..233
FT /note="Cyclic nucleotide-binding"
FT /evidence="ECO:0000259|PROSITE:PS50042"
FT DOMAIN 265..378
FT /note="N-terminal Ras-GEF"
FT /evidence="ECO:0000259|PROSITE:PS50212"
FT DOMAIN 383..453
FT /note="PDZ"
FT /evidence="ECO:0000259|PROSITE:PS50106"
FT DOMAIN 604..690
FT /note="Ras-associating"
FT /evidence="ECO:0000259|PROSITE:PS50200"
FT DOMAIN 715..942
FT /note="Ras-GEF"
FT /evidence="ECO:0000259|PROSITE:PS50009"
FT REGION 68..97
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1000..1047
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1088..1174
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1219..1252
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1301..1355
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1389..1484
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 79..94
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1031..1045
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1101..1159
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1301..1331
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1334..1348
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1484 AA; 165088 MW; D1F8CEF6949D1194 CRC64;
MAFLVRCYAN CLQPWSSKLP ADFTKLHLTD SLHPQVTHVS SSHSGCSITS DSGSSSLSDI
YQATESEAGD MDLSGLPETA VDSEDDDDEE DIERASDPLM SRDIVRDCLE KDPIDRTDDD
IEQLLEFMHQ LPAFANMTMS VRRELCAVMV FAVVERAGTI VLNDGEELDS WSVILNGSVE
VTYPDGKAEI LCMGNSFGVS PTMDKEYMKG VMRTKVDDCQ FVCIAQQDYC RILNQVEKNM
QKVEEEGEIV MVKEHRELDR TGTRKGHIVI KGTSERLTMH LVEEHSVVDP TFIEDFLLTY
RTFLCSPMEV GKKLLEWFND PSLRDKVTRV VLLWVNNHFN DFEGDPAMTR FLEEFENNLE
REKMGGHLRL LNIACAAKAK RRLMTLTKPA REAPLPFILL GGSEKGFGIF VDSVDSASKA
TEAGLKRGDQ ILEVNGQNFE NIQLSKAMEI LRNNTHLSIT VKTNLFVFKE LLTRLSEEKR
NGAPHLPKIG DIKKASRYSI PDLAVDVEQV IGLEKVNKKS KANTVGGRNK LKKILDKTRI
SILPQKPYND IGIGQSQDDS IVGLRQTKHI PTALPVSGTL SSSNPDLLQS HHRILDFSAT
PDLPDQVLRV FKADQQSRYI MISKDTTAKE VVVQAIREFA VTATPDQYSL CEVSVTPEGV
IKQRRLPDQL SKLADRIQLS GRYYLKNNME TETLCSDEDA QELLRESQIS LLQLSTVEVA
TQLSMRNFEL FRNIEPTEYI DDLFKLKSKT SCANLKTFEE VINQETFWVA SEILRETNQL
KRMKIIKHFI KIALHCRECK NFNSMFAIIS GLNLAPVARL RTTWEKLPNK YEKLFQDLQD
LFDPSRNMAK YRNVLNSQNL QPPIIPLFPV IKKDLTFLHE GNDSKVDGLV NFEKLRMIAK
EIRHVGRMAS VNMDPALMFR TRKKKWRSLG SLSQGSTNAT VLDVAQTGGH KKRVRRSSFL
NAKKLYEDAQ MARKVKQYLS NLELEMDEES LQTLSLQCEP ATNTLPKNPT DKKPVKSETS
PVAPRAGLQP KAQPQPQPPQ PPHKLNQGLQ VPAVSLYPSR KKVPVKDLPP FGINSPQALK
KILSLSEEGS LERHRRPAED TISNTSSQLS SPPTSPQSSP RKGYTLAPSG TVDNFSDSGH
SEISSRSSIV SNSSFDSLPV SLPDERRQRP SVSIVETSLA SGRLERRPAV EPDQYSLGSC
APLSESRGLY AAATVISSPS TEELSQDQGD RASLDAADSG RGSWTSCSSG SHDNIQTIQH
QRSWETLPFG HTHFDYPGDA AGLWASSSHM DQIMFPDHSA KYSRQSQSRE SLDQAQSRAS
WASSTGYWGE DSEGDTGTIK RRGGKDVSLD ADSSSMTAVT AEEAKPAAMA AHIAVTPSAA
KGLIARKEGR YREPPPTPPG YVGIPITDFP EAHPHPARKP PDYTVALQRS RMLARPAEPP
APGSARPAPR PQWHRPGDGD PRAGPCAPPG LTAEEDEDEQ VSAV
//
