ID   A0A3Q2DD25_CYPVA        Unreviewed;       590 AA.
AC   A0A3Q2DD25;
DT   10-APR-2019, integrated into UniProtKB/TrEMBL.
DT   10-APR-2019, sequence version 1.
DT   27-MAR-2024, entry version 25.
DE   RecName: Full=Beta-galactosidase {ECO:0000256|RuleBase:RU000675};
DE            EC=3.2.1.23 {ECO:0000256|RuleBase:RU000675};
OS   Cyprinodon variegatus (Sheepshead minnow).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC   Ovalentaria; Atherinomorphae; Cyprinodontiformes; Cyprinodontidae;
OC   Cyprinodon.
OX   NCBI_TaxID=28743 {ECO:0000313|Ensembl:ENSCVAP00000016717.1, ECO:0000313|Proteomes:UP000265020};
RN   [1] {ECO:0000313|Ensembl:ENSCVAP00000016717.1}
RP   IDENTIFICATION.
RG   Ensembl;
RL   Submitted (NOV-2023) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC         in beta-D-galactosides.; EC=3.2.1.23;
CC         Evidence={ECO:0000256|RuleBase:RU000675};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC       {ECO:0000256|ARBA:ARBA00009809, ECO:0000256|RuleBase:RU003679}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   AlphaFoldDB; A0A3Q2DD25; -.
DR   STRING; 28743.ENSCVAP00000016717; -.
DR   Ensembl; ENSCVAT00000025184.1; ENSCVAP00000016717.1; ENSCVAG00000019805.1.
DR   GeneTree; ENSGT00950000182942; -.
DR   OMA; ILIMEYW; -.
DR   Proteomes; UP000265020; Unplaced.
DR   GO; GO:0097546; C:ciliary base; IEA:Ensembl.
DR   GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0060271; P:cilium assembly; IEA:Ensembl.
DR   Gene3D; 2.60.120.260; Galactose-binding domain-like; 2.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   InterPro; IPR026283; B-gal_1-like.
DR   InterPro; IPR048912; BetaGal1-like_ABD1.
DR   InterPro; IPR048913; BetaGal_gal-bd.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR   InterPro; IPR019801; Glyco_hydro_35_CS.
DR   InterPro; IPR001944; Glycoside_Hdrlase_35.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR23421:SF199; BETA-GALACTOSIDASE; 1.
DR   PANTHER; PTHR23421; BETA-GALACTOSIDASE RELATED; 1.
DR   Pfam; PF21317; BetaGal_ABD_1; 1.
DR   Pfam; PF21467; BetaGal_gal-bd; 1.
DR   Pfam; PF01301; Glyco_hydro_35; 1.
DR   PIRSF; PIRSF006336; B-gal; 2.
DR   PRINTS; PR00742; GLHYDRLASE35.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR   PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU000675};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU000675};
KW   Reference proteome {ECO:0000313|Proteomes:UP000265020}.
FT   DOMAIN          13..326
FT                   /note="Glycoside hydrolase 35 catalytic"
FT                   /evidence="ECO:0000259|Pfam:PF01301"
FT   DOMAIN          384..484
FT                   /note="Beta-galactosidase 1-like first all-beta"
FT                   /evidence="ECO:0000259|Pfam:PF21317"
FT   DOMAIN          514..553
FT                   /note="Beta-galactosidase galactose-binding"
FT                   /evidence="ECO:0000259|Pfam:PF21467"
FT   ACT_SITE        162
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR006336-1"
FT   ACT_SITE        236
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR006336-1"
SQ   SEQUENCE   590 AA;  67704 MW;  70AEADA1F60ABEEF CRC64;
     MAHLEGLRAD SSQFTLRGQP FRILGGSIHY FRVPRPYWKD RLLKMKACGL NTLTTYVPWN
     LHEPERGAFN FQDQLDLRKA YVSLAAEIGF WVILRPGPYI CAEWDLGGLP SWLLQDKDMQ
     LRTTYPGFVN AVNVYFDKLV SVIQPLMFEN GGPVIALQLE NEYGSYAKDE EYMPFLKNCL
     RSRGINELLL TSDNWEGLRY KGMEGVLKTI NLQRLSFGAI QHLACMQPQK PLLVMEYWSG
     WFDVWGEHHH VFHAEDMLGV VSEILERGVS VNLYMFHGGT NFGFMNGAMD FGAYKPQVTS
     YDYDAPLSEA GGYTKKYEVL RDLFSRYNAE PLPEVPSPQE RKAYETVSTQ QNLSLWDSIQ
     FTEKPFRSER PINMENLPVN NNSGQSYGYT LYETSISSGG ALNSGNNIRD RALVFLDKQY
     VGCLDYKTHE IMLPDAERER TLTFLVENCG RVNYGKALDN QRKGIVGDIV LNDTCISGFN
     IFCLDLKPSF IKRFDSFIYF FYLLSNLIFL LHYPHDWGKG VVFVNGQNLG RYWCIGPQHF
     LYLPGPYLRS GENQIIVFEE EKVGGKILFS ENPEYGKTID VYKLPFCSLL
//