ID A0A3Q2I1N1_HORSE Unreviewed; 1404 AA.
AC A0A3Q2I1N1;
DT 10-APR-2019, integrated into UniProtKB/TrEMBL.
DT 13-SEP-2023, sequence version 2.
DT 27-MAR-2024, entry version 24.
DE SubName: Full=Patatin like phospholipase domain containing 7 {ECO:0000313|Ensembl:ENSECAP00000040937.2};
GN Name=PNPLA7 {ECO:0000313|Ensembl:ENSECAP00000040937.2,
GN ECO:0000313|VGNC:VGNC:21637};
OS Equus caballus (Horse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Perissodactyla; Equidae; Equus.
OX NCBI_TaxID=9796 {ECO:0000313|Ensembl:ENSECAP00000040937.2, ECO:0000313|Proteomes:UP000002281};
RN [1] {ECO:0000313|Ensembl:ENSECAP00000040937.2, ECO:0000313|Proteomes:UP000002281}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Thoroughbred {ECO:0000313|Ensembl:ENSECAP00000040937.2,
RC ECO:0000313|Proteomes:UP000002281};
RX PubMed=19892987; DOI=10.1126/science.1178158;
RG Broad Institute Genome Sequencing Platform;
RG Broad Institute Whole Genome Assembly Team;
RA Wade C.M., Giulotto E., Sigurdsson S., Zoli M., Gnerre S., Imsland F.,
RA Lear T.L., Adelson D.L., Bailey E., Bellone R.R., Bloecker H., Distl O.,
RA Edgar R.C., Garber M., Leeb T., Mauceli E., MacLeod J.N., Penedo M.C.T.,
RA Raison J.M., Sharpe T., Vogel J., Andersson L., Antczak D.F., Biagi T.,
RA Binns M.M., Chowdhary B.P., Coleman S.J., Della Valle G., Fryc S.,
RA Guerin G., Hasegawa T., Hill E.W., Jurka J., Kiialainen A., Lindgren G.,
RA Liu J., Magnani E., Mickelson J.R., Murray J., Nergadze S.G., Onofrio R.,
RA Pedroni S., Piras M.F., Raudsepp T., Rocchi M., Roeed K.H., Ryder O.A.,
RA Searle S., Skow L., Swinburne J.E., Syvaenen A.C., Tozaki T., Valberg S.J.,
RA Vaudin M., White J.R., Zody M.C., Lander E.S., Lindblad-Toh K.;
RT "Genome sequence, comparative analysis, and population genetics of the
RT domestic horse.";
RL Science 326:865-867(2009).
RN [2] {ECO:0000313|Ensembl:ENSECAP00000040937.2}
RP IDENTIFICATION.
RC STRAIN=Thoroughbred {ECO:0000313|Ensembl:ENSECAP00000040937.2};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = (9Z)-
CC octadecenoate + H(+) + sn-glycerol 3-phosphocholine;
CC Xref=Rhea:RHEA:40807, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16870, ChEBI:CHEBI:28610, ChEBI:CHEBI:30823;
CC Evidence={ECO:0000256|ARBA:ARBA00024569};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40808;
CC Evidence={ECO:0000256|ARBA:ARBA00024569};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-hexadecanoyl-sn-glycero-3-phosphate + H2O = H(+) +
CC hexadecanoate + sn-glycerol 3-phosphate; Xref=Rhea:RHEA:49092,
CC ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57518, ChEBI:CHEBI:57597;
CC Evidence={ECO:0000256|ARBA:ARBA00000355};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:49093;
CC Evidence={ECO:0000256|ARBA:ARBA00000355};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = H(+) +
CC hexadecanoate + sn-glycerol 3-phosphocholine; Xref=Rhea:RHEA:40435,
CC ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16870, ChEBI:CHEBI:72998;
CC Evidence={ECO:0000256|ARBA:ARBA00000597};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40436;
CC Evidence={ECO:0000256|ARBA:ARBA00000597};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1-acyl-sn-glycero-3-phosphocholine + H2O = a fatty acid +
CC H(+) + sn-glycerol 3-phosphocholine; Xref=Rhea:RHEA:15177,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16870,
CC ChEBI:CHEBI:28868, ChEBI:CHEBI:58168; EC=3.1.1.5;
CC Evidence={ECO:0000256|ARBA:ARBA00000150};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:15178;
CC Evidence={ECO:0000256|ARBA:ARBA00000150};
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000256|ARBA:ARBA00004643}; Single-pass type III membrane protein
CC {ECO:0000256|ARBA:ARBA00004643}. Membrane
CC {ECO:0000256|ARBA:ARBA00004183}; Single-pass type III membrane protein
CC {ECO:0000256|ARBA:ARBA00004183}.
CC -!- SIMILARITY: Belongs to the NTE family. {ECO:0000256|ARBA:ARBA00006636}.
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DR STRING; 9796.ENSECAP00000040937; -.
DR PaxDb; 9796-ENSECAP00000040937; -.
DR Ensembl; ENSECAT00000031343.2; ENSECAP00000040937.2; ENSECAG00000017449.4.
DR VGNC; VGNC:21637; PNPLA7.
DR GeneTree; ENSGT00940000156763; -.
DR InParanoid; A0A3Q2I1N1; -.
DR OMA; SSGYVWR; -.
DR Proteomes; UP000002281; Chromosome 25.
DR Bgee; ENSECAG00000017449; Expressed in gluteus medius and 21 other cell types or tissues.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005811; C:lipid droplet; IEA:Ensembl.
DR GO; GO:0004622; F:lysophospholipase activity; IBA:GO_Central.
DR GO; GO:0102545; F:phosphatidyl phospholipase B activity; IEA:UniProtKB-EC.
DR GO; GO:0034638; P:phosphatidylcholine catabolic process; IEA:Ensembl.
DR CDD; cd00038; CAP_ED; 3.
DR CDD; cd07225; Pat_PNPLA6_PNPLA7; 1.
DR Gene3D; 3.40.1090.10; Cytosolic phospholipase A2 catalytic domain; 2.
DR Gene3D; 2.60.120.10; Jelly Rolls; 3.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR000595; cNMP-bd_dom.
DR InterPro; IPR018490; cNMP-bd_dom_sf.
DR InterPro; IPR002641; PNPLA_dom.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR PANTHER; PTHR14226; NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER; 1.
DR PANTHER; PTHR14226:SF23; PATATIN-LIKE PHOSPHOLIPASE DOMAIN-CONTAINING PROTEIN 7; 1.
DR Pfam; PF00027; cNMP_binding; 3.
DR Pfam; PF01734; Patatin; 1.
DR SMART; SM00100; cNMP; 3.
DR SUPFAM; SSF51206; cAMP-binding domain-like; 3.
DR SUPFAM; SSF52151; FabD/lysophospholipase-like; 1.
DR PROSITE; PS50042; CNMP_BINDING_3; 3.
DR PROSITE; PS51635; PNPLA; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Lipid degradation {ECO:0000256|ARBA:ARBA00022963};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000002281};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 93..115
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 227..354
FT /note="Cyclic nucleotide-binding"
FT /evidence="ECO:0000259|PROSITE:PS50042"
FT DOMAIN 561..645
FT /note="Cyclic nucleotide-binding"
FT /evidence="ECO:0000259|PROSITE:PS50042"
FT DOMAIN 673..778
FT /note="Cyclic nucleotide-binding"
FT /evidence="ECO:0000259|PROSITE:PS50042"
FT DOMAIN 1010..1176
FT /note="PNPLA"
FT /evidence="ECO:0000259|PROSITE:PS51635"
FT REGION 402..474
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1357..1404
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 402..418
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1379..1404
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1404 AA; 154652 MW; E47F0AC5546681F1 CRC64;
MPVLTLPDCG VFLQAGFCLG ATLRSWVLQL MEQPSQAAMV TTSCSWETGR SQVLRPVSPL
AAPALLCPAC GQGWEGLHSG LANRCSVYDL AQLMGIAIGA LLALALVGVT VFFMYRKFSQ
FGQVQPTPQY RFRKRDKVMF YGRKIMRKVT TLPNTLVGNT AAPRQRVRKR TKVLSLAKRI
LRFKKEYPTL QPKEPPPSLL EADLTEFDVK NSHLPSEVLY MLKNVRVLGH FEKPLFLELC
KHMVFVQLLE GEHVFRPGEP DTSIYVVQDG RLEVCIQDTD GTEVAVKEVL AGDSVHSLLS
ILDVITGHTA PYKTVSARAA AASTVLRLPA AAFQGVFEKY PETLVRVVQI IMVRLQRVTF
LALHNYLGLT TELFNPESQA IPLVSVASLA VGKTKRQVSC SPEERLEKSL RPQESCDPDH
GGSRAAASGP LLKRSHSVPL PSVHKEISDE LGKAQAGDKA PSAPPGSVAG APSDLRMACD
RARVVLQAEE RPASAVASKS KKSVVVAETP SAVFHYEDST SDETAASRKT DAIFRAAKKD
LLTLMKLDDP SLLDGRVTLL HVPGGTVVSR QGDQDVNILF VVSGLLHVYQ RKIDSEEDTC
LFVTRPGEMV GQLAVLTGEP LIFTIKANRD CSFLSISKAH FYEIMRKQPT VVLGVAHTLV
KRMSSFVRQI DFALDWMEVE AGRAIYRQGD KSDCTYIVLS GRLRSVIRKD DGKKRLAGEY
GRGDLIGVVE TLTHQARATT VHAVRDSELA KLPAGALTSI KRRYPQVVTR LIHLLGEKIL
GSLQQGTATG HQFGLHAAGG KWDSGNPPSN LSTVAVMPVS EDVPLTTFAL ELKHALSAIG
PVLLLTSDNI KQRLGSAALD SIHEYRLSSW LGQQEDIHRI VLYQADSTLT PWTQRCIRQA
DCILIVGLGE QEPTVGELER MLESTAVRAQ KQLILLHREE GPAPSRTVEW LNMRSWCSGH
LHLCCPRRVF SRRSMPKLVE MYERVFQRPP DRHSDFSRLA RVLTGNAIAL VLGGGGARGC
AQVGIIRALV ECGIPVDMVG GTSIGAFMGA LYSEERSCSQ MRIRAKQWAE GMTSVVRTML
DLTYPITSMF SGAGFNNSIC SVFKDRQIED LWIPYFTITT DITASAMRVH TDGSLWRYVR
ASMSLSGYMP PLCDPKDGHL LMDGGYINNL PADVARSMGA KVVIAIDVGS QDEVDLTNYG
DALSGWWLLW KRWNPLATKV KVLNMAEIQT RLAYVCCVRQ LEMVKSSDYC ECLRPPIDRY
RTLDFGKFDE ICEVGYQHGR TVFDIWGRSG VLEKMLQDRQ GTSRMKACDA LTCPNASFTD
LAEIVSRIEP AKVAAVDDES DYQTEYEEEL LEGPQDAYAD FQCAPADSGS DSEDEPSLQH
QDPSLASAKL SRDSSSLCLS DQDG
//
