ID A0A3Q2ZT85_KRYMA Unreviewed; 922 AA.
AC A0A3Q2ZT85;
DT 10-APR-2019, integrated into UniProtKB/TrEMBL.
DT 10-APR-2019, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=DNA ligase {ECO:0000256|RuleBase:RU000617};
DE EC=6.5.1.1 {ECO:0000256|RuleBase:RU000617};
OS Kryptolebias marmoratus (Mangrove killifish) (Rivulus marmoratus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Atherinomorphae; Cyprinodontiformes; Rivulidae; Kryptolebias.
OX NCBI_TaxID=37003 {ECO:0000313|Ensembl:ENSKMAP00000001469.1, ECO:0000313|Proteomes:UP000264800};
RN [1] {ECO:0000313|Ensembl:ENSKMAP00000001469.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-
CC (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP +
CC diphosphate.; EC=6.5.1.1; Evidence={ECO:0000256|ARBA:ARBA00034003,
CC ECO:0000256|RuleBase:RU000617};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the ATP-dependent DNA ligase family.
CC {ECO:0000256|ARBA:ARBA00007572, ECO:0000256|RuleBase:RU004196}.
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DR RefSeq; XP_017292784.1; XM_017437295.1.
DR AlphaFoldDB; A0A3Q2ZT85; -.
DR STRING; 37003.ENSKMAP00000001469; -.
DR Ensembl; ENSKMAT00000001509.1; ENSKMAP00000001469.1; ENSKMAG00000001149.1.
DR GeneID; 108248493; -.
DR KEGG; kmr:108248493; -.
DR CTD; 3981; -.
DR GeneTree; ENSGT00860000133881; -.
DR OrthoDB; 8251at2759; -.
DR Proteomes; UP000264800; Unplaced.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003910; F:DNA ligase (ATP) activity; IEA:UniProtKB-EC.
DR GO; GO:0071897; P:DNA biosynthetic process; IEA:InterPro.
DR GO; GO:0051103; P:DNA ligation involved in DNA repair; IEA:InterPro.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; IEA:Ensembl.
DR CDD; cd07903; Adenylation_DNA_ligase_IV; 1.
DR CDD; cd17722; BRCT_DNA_ligase_IV_rpt1; 1.
DR CDD; cd17717; BRCT_DNA_ligase_IV_rpt2; 1.
DR CDD; cd07968; OBF_DNA_ligase_IV; 1.
DR Gene3D; 6.10.250.520; -; 1.
DR Gene3D; 3.40.50.10190; BRCT domain; 2.
DR Gene3D; 1.10.3260.10; DNA ligase, ATP-dependent, N-terminal domain; 1.
DR Gene3D; 3.30.470.30; DNA ligase/mRNA capping enzyme; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR InterPro; IPR044125; Adenylation_DNA_ligase_IV.
DR InterPro; IPR001357; BRCT_dom.
DR InterPro; IPR036420; BRCT_dom_sf.
DR InterPro; IPR000977; DNA_ligase_ATP-dep.
DR InterPro; IPR012309; DNA_ligase_ATP-dep_C.
DR InterPro; IPR012310; DNA_ligase_ATP-dep_cent.
DR InterPro; IPR016059; DNA_ligase_ATP-dep_CS.
DR InterPro; IPR012308; DNA_ligase_ATP-dep_N.
DR InterPro; IPR021536; DNA_ligase_IV_dom.
DR InterPro; IPR036599; DNA_ligase_N_sf.
DR InterPro; IPR029710; LIG4.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR NCBIfam; TIGR00574; dnl1; 1.
DR PANTHER; PTHR45997; DNA LIGASE 4; 1.
DR PANTHER; PTHR45997:SF1; DNA LIGASE 4; 1.
DR Pfam; PF00533; BRCT; 2.
DR Pfam; PF04679; DNA_ligase_A_C; 1.
DR Pfam; PF01068; DNA_ligase_A_M; 1.
DR Pfam; PF04675; DNA_ligase_A_N; 1.
DR Pfam; PF11411; DNA_ligase_IV; 1.
DR SMART; SM00292; BRCT; 2.
DR SUPFAM; SSF117018; ATP-dependent DNA ligase DNA-binding domain; 1.
DR SUPFAM; SSF52113; BRCT domain; 2.
DR SUPFAM; SSF56091; DNA ligase/mRNA capping enzyme, catalytic domain; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR PROSITE; PS50172; BRCT; 2.
DR PROSITE; PS00697; DNA_LIGASE_A1; 1.
DR PROSITE; PS00333; DNA_LIGASE_A2; 1.
DR PROSITE; PS50160; DNA_LIGASE_A3; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU000617};
KW DNA damage {ECO:0000256|RuleBase:RU000617};
KW DNA recombination {ECO:0000256|ARBA:ARBA00023172,
KW ECO:0000256|RuleBase:RU000617}; DNA repair {ECO:0000256|RuleBase:RU000617};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU000617};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU000617}; Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000264800};
KW Repeat {ECO:0000256|ARBA:ARBA00022737}.
FT DOMAIN 373..507
FT /note="ATP-dependent DNA ligase family profile"
FT /evidence="ECO:0000259|PROSITE:PS50160"
FT DOMAIN 671..760
FT /note="BRCT"
FT /evidence="ECO:0000259|PROSITE:PS50172"
FT DOMAIN 857..922
FT /note="BRCT"
FT /evidence="ECO:0000259|PROSITE:PS50172"
SQ SEQUENCE 922 AA; 104116 MW; 90762C45E421925D CRC64;
MFPLSFNKQS DFIMEGTSKS DAAGGPTVAD RVPFFHLCTT LEKIQRSKLR PEKSKILRDF
LESWRKFHSA LHKDDPKTTD SFYPAMRLIV PPFERERMAY GIKESMLAKL YIDVLGLPKN
GPEAGRLLNY RAPTTSQGEA GDFASMAYFV LKKRCSSQGG LSVKEVNDFL DSVAINNANK
QKDQVKKSLL HLITQSSALE QKWLIRMILK DMKLGISKET VLQVFHPDAA DLYNVNTDLK
KVCQQLHSPS VSLSEVSIEL FSAFKPMLAA VADIRGVEKQ MGGGAFYVET KLDGERIQLH
KDGDVYKYFS RNAFEYTQQF GSSPREGSLT PHVHGAFKGH VVSCILDGEM MAYDPAAETF
MQKGSKFDIK RLMDDSELQT CFCVFDVLLV NNQKLGKEPL KKRYETLQTV FSPVKGRLHL
VPKTEVRTMQ QVVGALNDAI DNREEGIMVK DPLSLYKPDK RGEGWLKIKP EYVDGLMDEL
DLLIVGGYWG KGRRGGMMSH FLCAVAEAPK PGEKPSVFHS LCRIGSGYTM KELYDLGLKL
AKHWKVYRKN DPPASILCGT EKPEVYIEPS DSVIIQVKAA EIVGSDMYKA NCTLRFPRIE
RIRDDKEWHQ CMTLAELEQF RGRASGKLAS RHVRVNGDEP QGKKRKLPAK PKKVIGIVDH
FKPQDLSGVS KETDMFEDVE FCVLNGTADH PKSELEKGVA RCCGIVVQNP GPDTYCVIAG
VENMRVKNLI SSNQHDVVWA SWLLECLDQR EVVPWQPRHM IHMSPSTREH FAKEYDAYGD
SYFVDTDERQ LREVFGRIGG AEAEAVNAAQ VEERYGWDDL PTSMFRPFTV YADVFADIGD
PTSVVPASCL NLRALEVRYH GGTVARTLEE GVSHVIVEEE TRLLDLRALR HRFRKKFKIV
RDSWVTDSIK AGYLLDDSDY LL
//
