ID A0A3Q3ETD7_KRYMA Unreviewed; 3910 AA.
AC A0A3Q3ETD7;
DT 10-APR-2019, integrated into UniProtKB/TrEMBL.
DT 10-APR-2019, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=DNA-dependent protein kinase catalytic subunit {ECO:0000256|ARBA:ARBA00018077};
DE EC=2.7.11.1 {ECO:0000256|ARBA:ARBA00012513};
GN Name=PRKDC {ECO:0000313|Ensembl:ENSKMAP00000005032.1};
OS Kryptolebias marmoratus (Mangrove killifish) (Rivulus marmoratus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Atherinomorphae; Cyprinodontiformes; Rivulidae; Kryptolebias.
OX NCBI_TaxID=37003 {ECO:0000313|Ensembl:ENSKMAP00000005032.1, ECO:0000313|Proteomes:UP000264800};
RN [1] {ECO:0000313|Ensembl:ENSKMAP00000005032.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC {ECO:0000256|ARBA:ARBA00004604}.
CC -!- SIMILARITY: Belongs to the PI3/PI4-kinase family.
CC {ECO:0000256|ARBA:ARBA00011031}.
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DR Ensembl; ENSKMAT00000005121.1; ENSKMAP00000005032.1; ENSKMAG00000000927.1.
DR GeneTree; ENSGT00940000155633; -.
DR Proteomes; UP000264800; Unplaced.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004677; F:DNA-dependent protein kinase activity; IEA:InterPro.
DR GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; IEA:InterPro.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd05172; PIKKc_DNA-PK; 1.
DR Gene3D; 1.10.1070.11; Phosphatidylinositol 3-/4-kinase, catalytic domain; 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR037706; DNA-PK_dom.
DR InterPro; IPR046804; DNA-PKcs_N.
DR InterPro; IPR046803; DNAPKcs_CC1-2.
DR InterPro; IPR012582; DNAPKcs_CC3.
DR InterPro; IPR045581; DNAPKcs_CC5.
DR InterPro; IPR003152; FATC_dom.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000403; PI3/4_kinase_cat_dom.
DR InterPro; IPR036940; PI3/4_kinase_cat_sf.
DR InterPro; IPR018936; PI3/4_kinase_CS.
DR InterPro; IPR003151; PIK-rel_kinase_FAT.
DR InterPro; IPR014009; PIK_FAT.
DR PANTHER; PTHR11139; ATAXIA TELANGIECTASIA MUTATED ATM -RELATED; 1.
DR PANTHER; PTHR11139:SF68; DNA-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT; 1.
DR Pfam; PF20500; DNA-PKcs_N; 1.
DR Pfam; PF20502; DNAPKcs_CC1-2; 1.
DR Pfam; PF08163; DNAPKcs_CC3; 1.
DR Pfam; PF19704; DNAPKcs_CC5; 1.
DR Pfam; PF02259; FAT; 2.
DR Pfam; PF02260; FATC; 1.
DR Pfam; PF00454; PI3_PI4_kinase; 1.
DR SMART; SM01343; FATC; 1.
DR SMART; SM01344; NUC194; 1.
DR SMART; SM00146; PI3Kc; 1.
DR SUPFAM; SSF48371; ARM repeat; 3.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR PROSITE; PS51189; FAT; 1.
DR PROSITE; PS51190; FATC; 1.
DR PROSITE; PS00915; PI3_4_KINASE_1; 1.
DR PROSITE; PS00916; PI3_4_KINASE_2; 1.
DR PROSITE; PS50290; PI3_4_KINASE_3; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW Kinase {ECO:0000256|ARBA:ARBA00022777};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000264800};
KW Serine/threonine-protein kinase {ECO:0000256|ARBA:ARBA00022527};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 2717..3325
FT /note="FAT"
FT /evidence="ECO:0000259|PROSITE:PS51189"
FT DOMAIN 3506..3835
FT /note="PI3K/PI4K catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50290"
FT DOMAIN 3878..3910
FT /note="FATC"
FT /evidence="ECO:0000259|PROSITE:PS51190"
FT COILED 2583..2610
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 3910 AA; 442593 MW; A28CA4403E98E0DA CRC64;
MCMVAYTKEK VAKCRTPLLE LLIKVLQTTK ASGVAADFRI GEIFNRFYGE LCQKNKLPDS
VLGKIYELLG VLAEVHPSEM VNNSDKLYKA YLGELKDQMT SSTKEPRLFV VAGCLRGLTA
LMVNFTKTME EDPGTSREIF QYALKAVTPQ VNQLSTGLRL LARHVSQFRG CLMDHYRVLF
ETMCKLCGHI NGEMKKTSYG ALEAFLKQVA LLVAENIEEH KAKLKFFMQK FCGIIKTMES
THKELSIAIR GYGFFAAPCK KVCPQDVDLM YTELIQRCKQ MYLTESDRED DSFYQLPSFL
DSIASVLIHL DKIPEVYTPL LERLLVVQID SFPQYSERMQ TACCRSVLKV LVAMASKGPV
LWSFISSVVH QGLIRVCSKP IVLSEVRHLL CIYTHIYLEN LFHPVTTTCR CFYLLFETCL
SFLLQDSGFL DGAFESQNAA LSSLRRLLYD ELVKSILRIV EKLDLSVQKI TTGEEAPDDS
AHVLLSSDPT AHLLPNKVKD FTAFINLVDF CSELLLNKHV EYFHSWMYPL SHELILHSIR
NPLVSGFYKL LSVTMKIAKR IKYFQGVGLR SSASPQGDTV KSACFALFSK FGKEVCVRMK
QYKDELLASC LTFVLSLHHN MAALDMKAFC PALETALRLG LSHVPLANAA LDALEDWSSN
IPQETMQSCY NQILPLLDGY LKTTSAVGES NWEVMSSLSS RSDRGYSRVM TRLLKKSNQL
STEDAPLVLL RLRVVKLLGH LGGKLNRNLV AAVVSSDEMM KKFVSWDSEK RLSFAVPFSD
MKPVIYLDPF LPRISELALS TSDRQTKVAA CELLHSLVVY MVGKSAQMVE GEDKLPPMYK
LHKRLFPVLL RLACDVDQVT RQLFEPLVMQ LIHWFTNNKK FESQDTVAVL EAILDGVVDP
MDSTLRDFCG RCIEEFVKWS IKQTTPKQQE RSPTNMKSLF KRIYSLALHP NGFKRLGAAL
AFNSIYRQFR EENSLVEQFV FEVLVIFVES LALAHSDERS TGTVQQCGSA IDHLKRIIKH
KASSLNQHNA KRRIPRGFPP DEKLFLSDVV VWLLEQCGRP QTECRHKCME LLYEFVPLLP
GKKSPPQWLD GVLKDRGVDF LISTFEGGGL LSQPTLRELS GPFSVRAALQ WMDLLLAVLD
CYNTFISLHI IKPQHVLNKS SFLKATRFFL TELAARELAA AESCFCLGEK TSHFSPREVE
QYNYSKCTIV VRLLEFASMI LVKGDQGFWK LLEQDLFSAV FFEVTAMVVC EPAAVGFNMA
DVEVMKNLPE VCVPLLKALL ASPYSSHLES SLRTKISRKS VEDLCAVDLY RSSSSHNQTE
MVLSSCKQIH RAGLLGSILL SQDSAHAESV GSKLLMTVYK GIAPGGDRKA LPSLDVNTKR
VADGLLQLAF SLSQQSEQLV DLLLNTIMLS VPISGGHSHN FTSFSHGEYF YSLFKATVNA
ELLRSLKSTV PRLMSAASQN PGMVSTLLNG MLDHSFRERS VRKTLGKELV DEVLGRWVSL
QLWWDGPSST SESKTATLLL LSKLLQIDSS VCSDVKHEAF RSVFSTFTSL LVDMNLPLNL
KSQALLVLPF FTTLQEEPLS ELQKALDALI ASHFPMQSDE FAKGSLHRNN YMDCVRKLLD
ALELTQSPLL LRLLAGVLCR DRKHIMEEQF QTCFQRIARR SVKALRLEGR VIGQGDVPVS
STLQAMTERV LLPLASHCSS KALSDFFVAN VCDILALLLS RFTKSSEAAF EVQLLKKIGC
LKLMELLYSR LPKDELYSKE SRINRAYCRS DTTEGNELSK TLIKSCFEAF TENMVGEAQL
LELRRLYHCA AYNCGIAAIS CSFSEPKFYQ GFLFSEKPEK NQFILENIVD VQKTYSFPIE
IEVPLERKKK YFMIRKEVGE ESGEAPVYLS SQSYMADSSL SEEMSQFDFS TGVQSFSFRG
QPDVSSVFRR QGTGMDELNQ HECMATMTAL IQHMQRNNIT PKPEQGGPPP ELPPWMKFLH
GKLANPSTPL NIRLFVSKLI INTEEVFRPY AKHWLGPLLQ LVVSGNNGGA GIHFMVVDVV
VTVLSWTSLA TPKENPRDEV LANRLLEFLM KHSFHAKRAV FRHNLEIIRT LVECWKDCLH
VPYSLIYERF CGTDPNSKDN SVGLQLLGII LANSLPAYEA SCGIDYDRYI QSLTCNLSFV
RYKEVYSAAA EVIGLILKHT AEMGNELLSL AATKITNLKK KDLDDKFIIC LNKVSKHFPP
FMDRFVNHVF FLLPKLHGVL KTLCLECVLS RADVIPDIFL QLQSRGFTQM MNHRDEARQR
VCLDVIHKIT ARLNPAELQE LLGPVTAFTS HPSPVCRERM YEILMWIQDN YGDEESMKDD
VSMEILTAAK EALLQGLSEE NQGLQLFVRN FWSQEKRLPT STLERMLAVL RSLYSCQIER
CFLSLATNLL LEMTSQSPDF RRNIFEYPLS ECTFQDCVID SNWRFRSTVM TPMFVETQSS
QGSESAAVAA SQSGFMKGKL RATQTSLEFS QTQTPGRRTA YNWLTGSSVD TLADYSLSSE
SLSSLLVFDK KTERQAAARR PVGEGFGLRR LTALADEVDS HTRGRNILRL RRRFLKDQEK
VSLGFAQKEI QQQRQKKEEK ADQRLRKEAQ VTLYRSYRSG DFPDVQIPFS SLIAPLQALA
QRDPILAKQL FCSLFAGILR EMEKELNPEE SRRIKEELRS NMNSFLSRST LCFPPFIACV
QDMCYQHREL QQLDPAAVSS TCLVSLQQPS GILLLEEGLL QAAGPEERPA KRSRGRREVP
PDTNKWIHLA RLYRSLEDYD VVRGIFGGKV GTKSITCAAL QAEANTDFAE AVKLYNEALN
TEDWPEGEPT DSEKDFWEIA AMEAYSQLTE WKSLQYCSTV YIDENSPPNL EKMWSEPLFQ
EMYLRYMICS MLKQLQLGES DQTLLSFIDD AMKVEERKKL LESLYSRELS LLYILQEDYD
RAKFYTNHAM QLFMESYSSV DTLLTQSRLT VLQSVQALTE IQDFLLFIKG DGEIPAGNAY
CPPASPDQIS RLLKLDTASV SVSGQYGRKE PRGRGELSAP SHPVELIRSC PCVQKNFPVA
TKLLKELHRE AKTNRGWLLP WVHCFSSYNH KRSQTQDPVE QIGAMLKTIP QLGTKNTGMF
RHQKILQGTS FHILASAVSR RPSVLGTLDR DKTERVVQLS GASPTPELGL QLRALKLLRE
ATRKADEELQ SYAQDFVDTG GVVDTYMALA NFCDKLLRDE EQSDVLALPV HVVESMLKAL
KMNSEEARLK FPRLLQIIEL YPSETLDLLT KEMPSVPCWI LISWISQMVA LLDKPEAVAV
QRSVGQIAEL YPQALVYALM ISSESYQFED SATGHQQLEF VNSMLDKGGA VRNFVDALQQ
LTNPDMIFRD WWDGVKNELD KPTFDQKQMS DMYKKMRSML GDRSTEGHGM FRKKFIQKFS
KDVEKLLGPD GSKLFEKKKD KHFLGQVDNL IKTMSDFQKE PGNLKEYSPW LSGFKADPFG
NELEVPGQYD GRSKPLPEYH AKITGFDERV KVMSSIRHPK RLIVRGDDER DHPFLVKGGE
DLRQDQRIEQ LFSVMNILLS HDTTCTNRGL QLRTYQVIPI STRIGLIEWM ENTCTLKDFL
YNTMTDEEQQ RARGCVVMYS RWLSTFGSAN ANVVACYGPS YRKAKRAEAV NNFLKVQQQV
PSDLLKRSFL KMCSSPEAFL SLRSHFISSH ALLCVSHWVL GIGDRHLSNF MVNMETGGMI
GIDFGHAFGS ATQFLPVPEL MPFRLTQQFV SLMQPLKESG LIQSIMVHSL RAYRAEPDLL
LNTMDVFVKE PSLDWKNFEL KQLKKGGTWT ESVNTKEINW FPLQKVNFAR RKLDGANPAA
ITSEELKLGF EKDAAFPGMQ SVALGSEEHN VRAKLPAEGL SVEKQVDCLL DQATDPNVLG
RAWMGWEPWL
//
