ID A0A3Q3GYZ0_9LABR Unreviewed; 389 AA.
AC A0A3Q3GYZ0;
DT 10-APR-2019, integrated into UniProtKB/TrEMBL.
DT 10-APR-2019, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=tRNA wybutosine-synthesizing protein 2 homolog {ECO:0000256|ARBA:ARBA00017179};
DE EC=2.5.1.114 {ECO:0000256|ARBA:ARBA00012265};
DE AltName: Full=tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase {ECO:0000256|ARBA:ARBA00031315};
OS Labrus bergylta (ballan wrasse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Eupercaria; Labriformes; Labridae; Labrus.
OX NCBI_TaxID=56723 {ECO:0000313|Ensembl:ENSLBEP00000038506.1, ECO:0000313|Proteomes:UP000261660};
RN [1] {ECO:0000313|Ensembl:ENSLBEP00000038506.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: S-adenosyl-L-methionine-dependent transferase that acts as a
CC component of the wybutosine biosynthesis pathway. Wybutosine is a hyper
CC modified guanosine with a tricyclic base found at the 3'-position
CC adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes
CC the transfer of the alpha-amino-alpha-carboxypropyl (acp) group from S-
CC adenosyl-L-methionine to the C-7 position of 4-demethylwyosine (imG-14)
CC to produce wybutosine-86. {ECO:0000256|ARBA:ARBA00037786}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-demethylwyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine =
CC 4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) +
CC H(+) + S-methyl-5'-thioadenosine; Xref=Rhea:RHEA:36355, Rhea:RHEA-
CC COMP:10164, Rhea:RHEA-COMP:10378, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17509, ChEBI:CHEBI:59789, ChEBI:CHEBI:64315,
CC ChEBI:CHEBI:73550; EC=2.5.1.114;
CC Evidence={ECO:0000256|ARBA:ARBA00036405};
CC -!- PATHWAY: tRNA modification; wybutosine-tRNA(Phe) biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004797}.
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DR AlphaFoldDB; A0A3Q3GYZ0; -.
DR STRING; 56723.ENSLBEP00000038506; -.
DR Ensembl; ENSLBET00000040099.1; ENSLBEP00000038506.1; ENSLBEG00000028712.1.
DR GeneTree; ENSGT00940000153304; -.
DR InParanoid; A0A3Q3GYZ0; -.
DR Proteomes; UP000261660; Unplaced.
DR GO; GO:0102522; F:tRNA 4-demethylwyosine alpha-amino-alpha-carboxypropyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006400; P:tRNA modification; IEA:UniProt.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 3.30.300.110; Met-10+ protein-like domains; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR030382; MeTrfase_TRM5/TYW2.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR23245; TRNA METHYLTRANSFERASE; 1.
DR PANTHER; PTHR23245:SF25; TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 2 HOMOLOG; 1.
DR Pfam; PF02475; Met_10; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51684; SAM_MT_TRM5_TYW2; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000261660};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 108..388
FT /note="SAM-dependent methyltransferase TRM5/TYW2-type"
FT /evidence="ECO:0000259|PROSITE:PS51684"
SQ SEQUENCE 389 AA; 43429 MW; 96A7550390268A7E CRC64;
MDGIPFLRVS QCNAQKLRKR LQSKGALDLS LCVMKDSDET VLLPISPSCV SHDLLLSLSC
FFPQSPLLPY KNKGKPSGNK LEKILQELFQ CHGERWSEEL RADLPRSFQR HGDLVLLGDN
SFNLPLWKKM GTLWSAVAKG LGAQRLAKIS RISGDGFRSP MVTMLLGEHS WVNIYEFDVT
KCMFSAGNIT EKLRVAAFDC RGQTVVDLYA GIGYFTLPYL VHAGAHHVHA CEWNPDAVEA
LKKNLVSNRV SDRCTVHQGD NRQLQLSDVA DRVNLGLIPS SEDGWPVACR LLKKSTGGIL
HIHQNVTSPF SNTANFPATD DATTLRISGR TSDREIWQAW ADDTANRIRC HLKDISGTTW
ITNIQHIEHV KSYAPHVNHV VLDLECRPA
//
