ID A0A3Q3MXB8_9TELE Unreviewed; 1637 AA.
AC A0A3Q3MXB8;
DT 10-APR-2019, integrated into UniProtKB/TrEMBL.
DT 02-JUN-2021, sequence version 2.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=Rap guanine nucleotide exchange factor 2 {ECO:0000256|ARBA:ARBA00016709};
DE AltName: Full=Cyclic nucleotide ras GEF {ECO:0000256|ARBA:ARBA00031545};
DE AltName: Full=Neural RAP guanine nucleotide exchange protein {ECO:0000256|ARBA:ARBA00032021};
DE AltName: Full=PDZ domain-containing guanine nucleotide exchange factor 1 {ECO:0000256|ARBA:ARBA00030673};
DE AltName: Full=RA-GEF-1 {ECO:0000256|ARBA:ARBA00029925};
DE AltName: Full=Ras/Rap1-associating GEF-1 {ECO:0000256|ARBA:ARBA00031980};
GN Name=RAPGEF2 {ECO:0000313|Ensembl:ENSMAMP00000024874.2};
OS Mastacembelus armatus (zig-zag eel).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Anabantaria; Synbranchiformes; Mastacembelidae; Mastacembelus.
OX NCBI_TaxID=205130 {ECO:0000313|Ensembl:ENSMAMP00000024874.2, ECO:0000313|Proteomes:UP000261640};
RN [1] {ECO:0000313|Ensembl:ENSMAMP00000024874.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- SUBCELLULAR LOCATION: Cell junction {ECO:0000256|ARBA:ARBA00004282}.
CC Cell membrane {ECO:0000256|ARBA:ARBA00004236}. Cytoplasm, perinuclear
CC region {ECO:0000256|ARBA:ARBA00004556}. Endosome
CC {ECO:0000256|ARBA:ARBA00004177}. Late endosome
CC {ECO:0000256|ARBA:ARBA00004603}.
CC -!- SIMILARITY: Belongs to the RAPGEF2 family.
CC {ECO:0000256|ARBA:ARBA00010829}.
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DR Ensembl; ENSMAMT00000025516.2; ENSMAMP00000024874.2; ENSMAMG00000016541.2.
DR GeneTree; ENSGT00940000156418; -.
DR Proteomes; UP000261640; Unplaced.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-SubCell.
DR GO; GO:0005770; C:late endosome; IEA:UniProtKB-SubCell.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR CDD; cd00038; CAP_ED; 2.
DR CDD; cd00992; PDZ_signaling; 1.
DR CDD; cd01785; RA_PDZ-GEF1; 1.
DR CDD; cd00155; RasGEF; 1.
DR CDD; cd06224; REM; 1.
DR Gene3D; 2.30.42.10; -; 1.
DR Gene3D; 2.60.120.10; Jelly Rolls; 2.
DR Gene3D; 1.10.840.10; Ras guanine-nucleotide exchange factors catalytic domain; 1.
DR Gene3D; 1.20.870.10; Son of sevenless (SoS) protein Chain: S domain 1; 1.
DR InterPro; IPR000595; cNMP-bd_dom.
DR InterPro; IPR018490; cNMP-bd_dom_sf.
DR InterPro; IPR001478; PDZ.
DR InterPro; IPR036034; PDZ_sf.
DR InterPro; IPR000159; RA_dom.
DR InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR InterPro; IPR023578; Ras_GEF_dom_sf.
DR InterPro; IPR001895; RASGEF_cat_dom.
DR InterPro; IPR036964; RASGEF_cat_dom_sf.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR45161; CYTOSKELETON-ASSOCIATED PROTEIN 4; 1.
DR PANTHER; PTHR45161:SF2; RAP GUANINE NUCLEOTIDE EXCHANGE FACTOR 2; 1.
DR Pfam; PF00595; PDZ; 1.
DR Pfam; PF00788; RA; 1.
DR Pfam; PF00617; RasGEF; 1.
DR Pfam; PF00618; RasGEF_N; 1.
DR SMART; SM00100; cNMP; 2.
DR SMART; SM00228; PDZ; 1.
DR SMART; SM00314; RA; 1.
DR SMART; SM00147; RasGEF; 1.
DR SMART; SM00229; RasGEFN; 1.
DR SUPFAM; SSF51206; cAMP-binding domain-like; 2.
DR SUPFAM; SSF50156; PDZ domain-like; 1.
DR SUPFAM; SSF48366; Ras GEF; 1.
DR SUPFAM; SSF54236; Ubiquitin-like; 1.
DR PROSITE; PS50042; CNMP_BINDING_3; 2.
DR PROSITE; PS50106; PDZ; 1.
DR PROSITE; PS50200; RA; 1.
DR PROSITE; PS50009; RASGEF_CAT; 1.
DR PROSITE; PS50212; RASGEF_NTER; 1.
PE 3: Inferred from homology;
KW Cell junction {ECO:0000256|ARBA:ARBA00022949};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Differentiation {ECO:0000256|ARBA:ARBA00022782};
KW Endosome {ECO:0000256|ARBA:ARBA00022753};
KW Guanine-nucleotide releasing factor {ECO:0000256|ARBA:ARBA00022658,
KW ECO:0000256|PROSITE-ProRule:PRU00168};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Neurogenesis {ECO:0000256|ARBA:ARBA00022902};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000261640};
KW Ubl conjugation {ECO:0000256|ARBA:ARBA00022843}.
FT DOMAIN 37..84
FT /note="Cyclic nucleotide-binding"
FT /evidence="ECO:0000259|PROSITE:PS50042"
FT DOMAIN 290..355
FT /note="Cyclic nucleotide-binding"
FT /evidence="ECO:0000259|PROSITE:PS50042"
FT DOMAIN 422..536
FT /note="N-terminal Ras-GEF"
FT /evidence="ECO:0000259|PROSITE:PS50212"
FT DOMAIN 540..610
FT /note="PDZ"
FT /evidence="ECO:0000259|PROSITE:PS50106"
FT DOMAIN 771..857
FT /note="Ras-associating"
FT /evidence="ECO:0000259|PROSITE:PS50200"
FT DOMAIN 882..1109
FT /note="Ras-GEF"
FT /evidence="ECO:0000259|PROSITE:PS50009"
FT REGION 195..254
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 635..656
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1169..1202
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1242..1329
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1365..1402
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1452..1637
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 195..220
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 236..251
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1186..1201
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1256..1279
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1287..1307
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1386..1402
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1469..1483
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1485..1510
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1532..1583
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1637 AA; 182166 MW; CA14AC3AC25EAB47 CRC64;
MASYVDNSFR QAVMMNPAER TQQDLEIVYS YLHGMEALSN LREHQLRMMC ETVRYERHEA
NEVLYYPDDI GSCWYILLSG SVFIKESMFL PRSSFGKRSA GSLRRGCECI VLEPSEMIVV
DYMDENEEYF QRQASHRQSR RRFRKINQKG ERQTIIDTVD PYPTGKPPIA RGYHTLPADF
SRLHLADGLH PQVTHVSSSH SGCSITSDSG SSSLSDIYQA TENEPGDMDL SGLPETAVDS
EEDDDEEDIE RASDPLMSRD IVRDCLEKDP MDRTDDDIEQ LLEFMHQLPA FANMTMSVRR
ELCAVMVFAV VERAGTIVLN DGEELDSWSV ILNGSVEVTY PDSRTEILCM GNSFGVSPTM
EKEYMKGVMK TKVDDCQFVC IAQQDYCCIL NQVEKNMQKV EEEGEIVMVK EHRELDRTGT
RKGHIVIKGT PERLTMHLVE EHSVVDPTYI EDFLLTYRTF LSSPMVVGKK LLEWFHDPSL
RDKVTRVVLL WVNNHFNDFE GDPAMTHFLE EFENNLEKEK MCGHLRLLNI ACAAKAKPRQ
VTLTKPSRDS PLAFSLLGGQ EKGFRIFIDA VDPGSKAAEA GLKRGDQILE VNGQNFENVQ
LSKANEILKN NTHLSITVKT NLLVFKELLT RPEQDHDLDG EDEHDRKNGA PHLPKIGDIK
KASRYSIPDL AVDVEQVMGL EKVSKKAKTN TVGGRNKLKK IFDKTLTSIL PPKPYNDVCV
GQSQDDSIVG MKQSKQIPPA LPVSGNLSSS NPDLLQSHHR ILDFNNQPDM SDQVLRVFKA
DQQSRYIMIG KDTTAKEVVA QAIREFALTA APEAYSLCEV SVTPEGVIKQ RRLPDQLSKL
ADRIQLSGRY YLKSNMETET LCSDEDAQDL LREGQISLLQ LSTVEVATQL SMRAFELFCA
IEPTEYIDDL FKLRSKTGSA SLKRFEEAIN HETFWVATEV TREPNQLKRM KTIKHFIKIA
LHCRECKNFN SMFAIISGLN LAPVSRLRGT WEKLPSKYEK LFGDLQDLFD PSRNMAKYRN
VLNNQNLQPP IIPLFPVIKK DLTFLHEGND SKVDGLVNFE KLRMIAKEIR HVGRMASVNM
DPALMFRTRK KKWRSLGSLS QGSANAAVLD VTQTGGHKKR VRRSSFLNAK KLYEDAQMAR
KVKQYLSNLS LETNEESLQT LSLQCEPSIS TLPKNAGGKR PDTSPVVSRA ANQQRGQLAK
GNQALQVPAV ALYPSRKKVP VKDLPPFGTS SPQSLKKILS LSEEGSDRHR RQPEDTVSNA
SSQLFSPPTS PQSSPKKGYR MGDTYSDSGH SEISSRSSLV SNSSFDMAQE ERRLRHSGGI
GEAHIGGARL ERRATTDPDQ YSLGSYSSMQ DCRGIYVGCP TVLSSPSSEE LTQDQGDRVS
LDAADSGRGS WTSCSSGSHD NIQTMQQGRS WETLAFGVGG GGIGGLPPGG PEALLGGPAA
LWAAQARGSW ASASSSSSSA AYWGEDSEGD TGTIKRRGGK DVSADPETSS ITSTGSEEAK
QHSRPSPSPI TTGARKEGRY REPPPTPPGY TALSISDLSE GQHPVPSVPT STVTHSGRRP
PDYTTALQRS RMVTQSPDSH QAHQVAKQRV GGLHRTRSPA EEQGPEEEEE GESLSSKVIA
LKKPKPLAQH TPETPRQ
//
