ID A0A3Q3WT20_MOLML Unreviewed; 735 AA.
AC A0A3Q3WT20;
DT 10-APR-2019, integrated into UniProtKB/TrEMBL.
DT 10-APR-2019, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=E3 ubiquitin-protein ligase {ECO:0000256|PIRNR:PIRNR001569};
DE EC=2.3.2.26 {ECO:0000256|PIRNR:PIRNR001569};
OS Mola mola (Ocean sunfish) (Tetraodon mola).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Eupercaria; Tetraodontiformes; Molidae; Mola.
OX NCBI_TaxID=94237 {ECO:0000313|Ensembl:ENSMMOP00000012334.1, ECO:0000313|Proteomes:UP000261620};
RN [1] {ECO:0000313|Ensembl:ENSMMOP00000012334.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885,
CC ECO:0000256|PIRNR:PIRNR001569};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906, ECO:0000256|PIRNR:PIRNR001569}.
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DR AlphaFoldDB; A0A3Q3WT20; -.
DR STRING; 94237.ENSMMOP00000012334; -.
DR Ensembl; ENSMMOT00000012538.1; ENSMMOP00000012334.1; ENSMMOG00000009485.1.
DR OMA; LIFLICE; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000261620; Unplaced.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:InterPro.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro.
DR CDD; cd08382; C2_Smurf-like; 1.
DR CDD; cd00078; HECTc; 1.
DR CDD; cd00201; WW; 3.
DR Gene3D; 2.20.70.10; -; 2.
DR Gene3D; 2.60.40.150; C2 domain; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR024928; E3_ub_ligase_SMURF1.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR001202; WW_dom.
DR InterPro; IPR036020; WW_dom_sf.
DR PANTHER; PTHR11254:SF300; E3 UBIQUITIN-PROTEIN LIGASE SMURF2; 1.
DR PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR Pfam; PF00168; C2; 1.
DR Pfam; PF00632; HECT; 1.
DR Pfam; PF00397; WW; 3.
DR PIRSF; PIRSF001569; E3_ub_ligase_SMURF1; 3.
DR SMART; SM00239; C2; 1.
DR SMART; SM00119; HECTc; 1.
DR SMART; SM00456; WW; 3.
DR SUPFAM; SSF49562; C2 domain (Calcium/lipid-binding domain, CaLB); 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR SUPFAM; SSF51045; WW domain; 3.
DR PROSITE; PS50004; C2; 1.
DR PROSITE; PS50237; HECT; 1.
DR PROSITE; PS01159; WW_DOMAIN_1; 1.
DR PROSITE; PS50020; WW_DOMAIN_2; 3.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000261620};
KW Transferase {ECO:0000256|PIRNR:PIRNR001569};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PIRNR:PIRNR001569}.
FT DOMAIN 1..98
FT /note="C2"
FT /evidence="ECO:0000259|PROSITE:PS50004"
FT DOMAIN 138..171
FT /note="WW"
FT /evidence="ECO:0000259|PROSITE:PS50020"
FT DOMAIN 236..269
FT /note="WW"
FT /evidence="ECO:0000259|PROSITE:PS50020"
FT DOMAIN 282..315
FT /note="WW"
FT /evidence="ECO:0000259|PROSITE:PS50020"
FT DOMAIN 401..735
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT ACT_SITE 703
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001569-1,
FT ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 735 AA; 84404 MW; 6FC51C9631DC47B6 CRC64;
CAKNLVKKDF FRLPDPFAKV VVDGSGQCHS TDTVRNTLDP KWNQHYDLYI GKSDSITISV
WNHKKIHKKQ GAGFLGCVRL LSNAINRLKD TGYQRLDLNK LSPNDSDTVR GQIVVSLQSR
DRIGAGGPVV DCSRLFDNDL PDGWEERSTA SGRIQYLNHI TRTTQWERPT RPASEYSSPG
RPLSCIVDEN TPISTNGATP TTVMPASGID QRVQERRVRS QRHRNYMSRT HLHTPPDLPE
GYEQRTTQQG QVYFLHTQTG VSTWHDPRVP RDLSNVNCEE LGPLPPGWEI RNTATGRVYF
VDHNNRTTQF TDPRLSANLH LVLNRQNQLK DQAQQALVSP GQLPEEAECL TVPKYKRDLV
QKLKILRQEL SQQQPQAGHC RIEVSREEIF EESYRQVMKM RPKDLWKRLM VKFRGEEGLD
YGGVAREWLY LLSHEMLNPY YGLFQYSRDD IYTLQINPDS AVNPEHLSYF HFVGRIMGMA
VFHGHYIDGG FTLPFYKQLL GKPITLDDME SVDPDLHNSL VWILDNDITG VLDHTFCVEH
NAYGEIIQHE LKPNGKSIPV TQDTKKEYVR LYVNWRFLRG IEAQFLALQK GFNEVIPQHL
LKAFDEKELE LIVCGLGKID INDWKSNTRL KHCTPDSNIV KWFWKAVESF DEERRARLLQ
FVTGSSRVPL QGFKALQGAA GPRLFTIHQI DASTNNLPKA HTCFNRIDIP PYEHYDKLYD
KLLTAIEETC GFAVE
//
