ID A0A3Q4M1U7_NEOBR Unreviewed; 1685 AA.
AC A0A3Q4M1U7;
DT 10-APR-2019, integrated into UniProtKB/TrEMBL.
DT 10-APR-2019, sequence version 1.
DT 24-JAN-2024, entry version 25.
DE RecName: Full=DNA-directed RNA polymerase subunit {ECO:0000256|RuleBase:RU004279};
DE EC=2.7.7.6 {ECO:0000256|RuleBase:RU004279};
GN Name=POLR1A {ECO:0000313|Ensembl:ENSNBRP00000000544.1};
OS Neolamprologus brichardi (Fairy cichlid) (Lamprologus brichardi).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Cichlomorphae; Cichliformes; Cichlidae; African cichlids;
OC Pseudocrenilabrinae; Lamprologini; Neolamprologus.
OX NCBI_TaxID=32507 {ECO:0000313|Ensembl:ENSNBRP00000000544.1, ECO:0000313|Proteomes:UP000261580};
RN [1] {ECO:0000313|Ensembl:ENSNBRP00000000544.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000256|RuleBase:RU004279}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550,
CC ECO:0000256|RuleBase:RU004279};
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC {ECO:0000256|ARBA:ARBA00006460, ECO:0000256|RuleBase:RU004279}.
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DR RefSeq; XP_006781957.1; XM_006781894.1.
DR STRING; 32507.ENSNBRP00000000544; -.
DR Ensembl; ENSNBRT00000000583.1; ENSNBRP00000000544.1; ENSNBRG00000000519.1.
DR GeneID; 102783638; -.
DR CTD; 25885; -.
DR GeneTree; ENSGT00920000149138; -.
DR OMA; NREDYQQ; -.
DR OrthoDB; 169836at2759; -.
DR Proteomes; UP000261580; Unplaced.
DR Bgee; ENSNBRG00000000519; Expressed in blood and 5 other cell types or tissues.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0031981; C:nuclear lumen; IEA:UniProt.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0048703; P:embryonic viscerocranium morphogenesis; IEA:Ensembl.
DR GO; GO:0014032; P:neural crest cell development; IEA:Ensembl.
DR GO; GO:0009303; P:rRNA transcription; IEA:Ensembl.
DR CDD; cd02735; RNAP_I_Rpa1_C; 1.
DR CDD; cd01435; RNAP_I_RPA1_N; 1.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.357.120; -; 1.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 3.30.70.2850; -; 1.
DR Gene3D; 6.10.250.2940; -; 1.
DR Gene3D; 3.30.1490.180; RNA polymerase ii; 1.
DR Gene3D; 4.10.860.120; RNA polymerase II, clamp domain; 1.
DR Gene3D; 1.10.274.100; RNA polymerase Rpb1, domain 3; 1.
DR InterPro; IPR047107; DNA-dir_RNA_pol1_lsu_C.
DR InterPro; IPR015699; DNA-dir_RNA_pol1_lsu_N.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; DNA-DIRECTED RNA POLYMERASE; 1.
DR PANTHER; PTHR19376:SF11; DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA1; 1.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR SMART; SM00663; RPOLA_N; 1.
DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000256|RuleBase:RU004279};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004279}; Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000261580};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU004279};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004279}.
FT DOMAIN 293..629
FT /note="RNA polymerase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00663"
FT REGION 1348..1467
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1370..1389
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1390..1404
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1405..1435
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1437..1461
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1685 AA; 189382 MW; 506B0B1DA27C8197 CRC64;
MLFGKEVPWR RLEGMSFGIY SAEEIRKLSV KAITNFRFLD GVGNVAPNGL YDLALGPADI
KEVCSTCCQD FNNCPGHLGH IELPLPVYNP LFFDKLYLLI RGSCLACHML TCPRAAMHLL
LNQLKLLDHG AMQEVYQIEQ VLGQYMEENV KATVDEVVQV LQVFTDATVG MNAATSENTK
PIKHLVEKKG SLINDFWKIH MKTRKCPHCG CGRSTVRHEH NSKLIVTMPS GAQTSDKTEE
DLLRGKRVYM TASTAREHVN KVWEKEGFFL KCLFSGLEEC MWSKEGGSSF YPDLFFLELL
VVPPCRYRPI NRLGDQMFTN GQTVNMQAVM KDCDVIRKLL ALIAGEKIVP ETEAVEQTDQ
SFLSGILGAT LTDKLYNVWV RLQSHVNIVF DSDMDKLMTE KYPGIRQILE KKDGLFRKHM
MGKRVDYAAR SVICPDMYIG TNEIGIPMVF ATKLTYPQPV TPWNVKELRQ AVLNGPNVHP
GASMVINEDG RKTILSPTCL AQREAVAKEL LTPSPGPRKM PMKIVNRHIK NGDVLLLNRQ
PTLHRPSIQA HCARILPGEK VLRLHYANCK AYNADFDGDE MNAHFPQSEL GRAEAYTLVS
TDQQYLVPKD GKPLAGLIQD HMVSGTRMTI RGCFFTKDQY TELVYRGLTD KPGRVKLLPP
AILKPQQLWT GKQVVSTLLL NVIPEKAVPL NLAGKSKIPS KAWIQVPPRA NPGYQPESMC
DSQVVIRQGE LLVGVLDKAH YGSSAYGLVH CCYELYGGET SGKLLSCLAR LFTAYLQLYR
GFTLGVEDIL VKLGANKRRK KIIKESLKIG TKALQAAFNL PSNVDQAEAQ SRWQDAHLNP
DQRDFITADH KFKEVANQVN NDINKVCMPV GLHTSFPDNN LQLMVQSGAK GSTVNTMQIS
CLLGQIELEG RRPPLMPSGK FLPCFQPYDP APGAGGFVSG RFLTGIKPQE FFFHCMAGRE
GLVDTAVKTS RSGYLQRCVI KHLEGLVVQY DLTVRDSDGS VVQFLYGEDG LDIPKTQFLQ
PRQFPFIEDN YEVIRRSQGL DEVLARLDPQ SATKHFAAIQ RWKAKRELAC SRSGAFLLFS
QKKLGKLKQT DEVEADCGRN PAVLQLVQQW HTLDEASRSK YCRKSSRCPE PSLGLFRPDV
CFGSISENFH GITEKYLQSR DTVNQDRLDT HSLRQLLHYK WQRSLCDPGE AVGLLAAQSI
GEPSTQMTLN TFHFAGRGEM NVTLGIPRLR EILMVASANI KTPMMSVPVL NNKRALKRVK
VLRKKLARVC LAEVLQKVGV VETLRIENHH KLRVFKITFH FLPPERYSDD RLLSPQQILH
YMETRFFRLL LEAIKKRSAK LASIAVETRR ATARDRDDDG NGAAASGGLD EGEEEQEREI
VDDQENEGDT DASDAKRKGK QEEEVDYESE EEDNGDDPED KEDQGVEEVE EETTAVEEPQ
ANGRRTEKRK KGEKRVEGAE SQSEMRVNSV LESNAAIERY SYDHKHELWC EVDLALPVSK
VHFDLTSVLT SLAQNAVIME TKGLTRCLLS ETTTKTGEKE TVLNTEGINM HEIFKYSDIL
DINRLYSNEV HAMAKTYGIE VALKVIEKEI KNVFAVYGIE VDPRHLSLVA DYMCYEGVYK
PLNRHAIRSN SSPLQQMTFE TSYKFLKQAT MLGSHDQLVS PSACLVVGKV VRGGTGLFEL
KQPLQ
//
