ID A0A3Q7S9E2_VULVU Unreviewed; 6904 AA.
AC A0A3Q7S9E2;
DT 10-APR-2019, integrated into UniProtKB/TrEMBL.
DT 10-APR-2019, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE SubName: Full=Nesprin-2 isoform X2 {ECO:0000313|RefSeq:XP_025856324.1};
GN Name=SYNE2 {ECO:0000313|RefSeq:XP_025856324.1};
OS Vulpes vulpes (Red fox).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Carnivora; Caniformia; Canidae; Vulpes.
OX NCBI_TaxID=9627 {ECO:0000313|Proteomes:UP000286640, ECO:0000313|RefSeq:XP_025856324.1};
RN [1]
RP IDENTIFICATION.
RG RefSeq;
RL Submitted (JAN-2019) to UniProtKB.
RN [2] {ECO:0000313|RefSeq:XP_025856324.1}
RP IDENTIFICATION.
RC STRAIN=TameXAggressive cross {ECO:0000313|RefSeq:XP_025856324.1};
RC TISSUE=Blood {ECO:0000313|RefSeq:XP_025856324.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- SUBCELLULAR LOCATION: Nucleus outer membrane
CC {ECO:0000256|ARBA:ARBA00004605}; Single-pass type IV membrane protein
CC {ECO:0000256|ARBA:ARBA00004605}; Cytoplasmic side
CC {ECO:0000256|ARBA:ARBA00004605}.
CC -!- SIMILARITY: Belongs to the nesprin family.
CC {ECO:0000256|ARBA:ARBA00008619}.
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DR RefSeq; XP_025856324.1; XM_026000539.1.
DR STRING; 9627.ENSVVUP00000040415; -.
DR OrthoDB; 5478539at2759; -.
DR Proteomes; UP000286640; Unplaced.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-UniRule.
DR GO; GO:0005635; C:nuclear envelope; IEA:UniProt.
DR GO; GO:0003779; F:actin binding; IEA:UniProtKB-KW.
DR GO; GO:0051641; P:cellular localization; IEA:UniProt.
DR CDD; cd21242; CH_SYNE2_rpt1; 1.
DR CDD; cd21244; CH_SYNE2_rpt2; 1.
DR CDD; cd00176; SPEC; 5.
DR Gene3D; 1.20.58.60; -; 13.
DR Gene3D; 1.10.418.10; Calponin-like domain; 2.
DR InterPro; IPR001589; Actinin_actin-bd_CS.
DR InterPro; IPR039906; Anc-1/SYNE1/2-like.
DR InterPro; IPR001715; CH_dom.
DR InterPro; IPR036872; CH_dom_sf.
DR InterPro; IPR012315; KASH.
DR InterPro; IPR018159; Spectrin/alpha-actinin.
DR InterPro; IPR002017; Spectrin_repeat.
DR PANTHER; PTHR21524; SPECTRIN REPEAT CONTAINING NUCLEAR ENVELOPE PROTEIN 2; 1.
DR PANTHER; PTHR21524:SF6; SPECTRIN REPEAT-CONTAINING NUCLEAR ENVELOPE PROTEIN 2; 1.
DR Pfam; PF00307; CH; 2.
DR Pfam; PF10541; KASH; 1.
DR Pfam; PF00435; Spectrin; 3.
DR SMART; SM00033; CH; 2.
DR SMART; SM01249; KASH; 1.
DR SMART; SM00150; SPEC; 20.
DR SUPFAM; SSF47576; Calponin-homology domain, CH-domain; 1.
DR SUPFAM; SSF46966; Spectrin repeat; 17.
DR PROSITE; PS00019; ACTININ_1; 1.
DR PROSITE; PS00020; ACTININ_2; 1.
DR PROSITE; PS50021; CH; 2.
DR PROSITE; PS51049; KASH; 1.
PE 3: Inferred from homology;
KW Actin-binding {ECO:0000256|ARBA:ARBA00023203};
KW Coiled coil {ECO:0000256|ARBA:ARBA00023054, ECO:0000256|SAM:Coils};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|PROSITE-
KW ProRule:PRU00385}; Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000286640};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|PROSITE-
KW ProRule:PRU00385}; Transmembrane helix {ECO:0000256|ARBA:ARBA00022989}.
FT TOPO_DOM 1..6853
FT /note="Cytoplasmic"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00385"
FT TOPO_DOM 6875..6904
FT /note="Perinuclear space"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00385"
FT DOMAIN 31..136
FT /note="Calponin-homology (CH)"
FT /evidence="ECO:0000259|PROSITE:PS50021"
FT DOMAIN 181..286
FT /note="Calponin-homology (CH)"
FT /evidence="ECO:0000259|PROSITE:PS50021"
FT DOMAIN 6845..6904
FT /note="KASH"
FT /evidence="ECO:0000259|PROSITE:PS51049"
FT REGION 1042..1086
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2359..2392
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 4073..4102
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 4124..4144
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 4415..4436
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 6340..6429
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 6512..6532
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 6780..6844
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 942..1035
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 1463..1490
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 1665..1699
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 2325..2359
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 3019..3060
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 3694..3721
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 3859..3907
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 3993..4061
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 4486..4513
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 4634..4683
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 5382..5433
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 5826..5860
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 1043..1086
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2364..2386
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4073..4087
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 6340..6377
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 6904 AA; 797979 MW; BC9D7B2A524B2B0A CRC64;
MASSPVPPTE DEQVSLGIDD RHISLQAEQE DTQKKTFTCW INSQLAKHIP PSVVSDLFAD
IKKGHVLLDL LEVLSGQQLP RDKGSNTFQC RINIEHALTF LKNRSIKLIN IHVTDIIDGN
PSIILGLIWT IILHFHIEEL ARTLSCNYNQ PSLDSVSVAD SSPTSSPPAK KCSRAQERWQ
MSAKKALLLW AQEQCAPYES VNVTDFKSSW RNGMAFLAVI HALRPDLIDM KSVKHRSNKD
NLQEAFGIAE REFKIPKLLE PEDVDVANPD EKSIMTYVAQ FLRYSKDAAE TGDKTQGKMK
DAVAWLTLQE KKLQKVLKDS ENETYSKKYE SLLSFLESFN EEKKPFLDIL PIKRIPDELS
EDQLQLREAW DGLNYQINVW KTELNHALPS PLHQIEAWLQ EIEGLIDEDL PASQDYCEAM
ALIQEKMTLI KNLMDKFDCN SNTLLTFENR DEEHLPLVPP KKLEEMKGRI NNISGKKLIP
RLEFHYYKCS VLGLIDEVKL KLDVWNVKYG NKASVELLLE DWHKFIEEKE FLAQLESSFQ
KCEEIHKNLA GQHQDISEQY TAVEHNICMY RKNIYNVKST LQKVLTCWAT YVENLRLLKA
CFEETKKEQM KEVPFETLSQ WNREHTTLNE VGNFLIQVSN DEVGSSISKE LRRLNKRWRK
FITKTQLDMN LPLIKKQDQP NFDNYENTQL SKEEKPTVDF STDMSIELPE NHNENIKAGE
EREKENEFTG QLKVAEEVEK LIGQMEIWEA ETKSLLDILR RQEGVDASVE ESLQHLIAKG
SMYEELVSRT EDTLQMDVQN ISSQESLQHV LTVGLQTKIQ EAKEKVQINM VKLIAVLKNS
TDASPNLDVR LRVEESQKEL ESYMTRAEQL LGQKESRSGL ISKYKEALII LNSKSLVKYL
KAVEELKNNV TEDVKLSLEE KSRSACAKWE PLHQEMSLYI QQLKIDIEKE KLNDNISKLE
KQINKEKKLI RRGRTKGLIK EHEACFSEEA SLYQLDHHMD VLRELCEELT SQKSQQEVRR
ALKDYEQKIE RLRKCASEIH MTLQPTMGGT SKNKETTNPS ENGGRDSHSE VPSVKSENQP
STEKAMDSIK NFSLESKLKP QPEENFMKYE KDHNAAVSSL QERHDMHREN LELYLEKNKF
RIISDFSSDK ERSSACLQDK LTDLRVLKNE IDACWKEFEI TSLKLEELDS DVKKPFISNM
RDKLKEKERE FQMTLNTRME SLETALKIVL PVEKESLLLC GSDLLLHELV IQEFHLIDAD
GIYQNLRNIQ DSIAKQVEIC NNVEQSGDFA LKDLYPVDVH ATQNITLKHR TQLEEANHKV
QRSKDALKAL EEFLASLRTA ELSFELSASD TQVVPEDTLT VENKKGEINL MKDQARHLDE
RLKMLGISFK DAERGDDTCD KLLDALSRKL FETHGYGVQE ELTEENKLLE TCILKNNELL
RNIQDVYNQI SKISLKDPTV PAVKQRKKSL MKLDKDLDEY EEEKKCLQEM VNSLPQFKDG
REKSLNQQFQ STVHLWDSTK TSVTECLDQC ERVLELLKQY QNFKSVLTAL IQKEESVISL
QASYMGKENL KKRIAEIEIV KEEFNEHLEV VDKINQICRN LQFHLNKMRI FEEPPFEKEA
NIIVDRWLDI NEKTEDYCEN LGRALALWDK LFNLKNVIDE WTEKVLQKME LHQVAEEERE
KLKEELQVHE QKSSEFSKRV AKIQFLLQSS EMPLELQVME SSILNKLENV KMNLIGESNC
SALSGSTAEL REDLDQAKTQ MGMTESLLKA LSPSDSLEIF TKLEEIQQQI LQQKHSMMLL
ENQIGCLTPE LSELKKQYES VSDLFNTKKS VLQDHFSKLL NDQCKNFNDW FGNIKMNLKE
CFELPETKKN VEQKLQKLSD FLTLEGRGSE IQQVGTVLNH VKKHLPKAHV KELNSWIINQ
EIELEKMDSI CQTQAKELND HLQQLLRLQD DHRNLSKWLT SQEEKWIEME KSGEKTELFC
EAVAGKREQF ESMAQLNSSL KEYGLTEEEE IIMESTHLID RYQTFLRQLC EIDEEDKLPP
TEDQSFNDLA HDVIHWIAGI KESLMVLNSS EGKMPLEERI QKIKEIILLK PEGDAKIQTI
MSQAESSKAP LAPKTLTDIK NQWDNTLHLA NTYLSHQEKL RLEGEKYLQS KEDLRLMLTE
LKKQQEAGFA LQHGLQEKKA QLKIYKKFLQ KAQDLTSLLK ELKSQGSYLL ECTKNPNFSE
KPWLEIKHLH ESLLQQLQDS VQKLEGHVQE HHSYQVCVTD LNTALDDISK EFASFSDKPV
DQIAVEEKVQ KLQELENRLR LQDGILKKIL ALAKSIKQNT SSGGQKIIKD DIKSLKCKQK
DLENRLECAK QESENYLNSI LKSERSTEQK EKFPLPGREK EVTSDGQEPT QDSAALEKFE
EDREINKNSA VEVVLSKQLS LNVQESMKNT EDEHKVNELQ NQPLELDVML RNEQLKEIEE
LYAQLEAKKI ATEPLEQTEC LNKAEASALV LHSTRYSVHH LDSLLQALIT LKKKKESQYE
LLNNFQEHLA AVESSVKALL MEKESLKVGP LDSATYLDKI KQFLASIEKE KDSLSRMKTE
WENVSNYLTD MDKKLLESQI KQLVNSWEQV EQLAQKKYSQ QVVEHDEFTL LMNQIQDLGI
SIQQQQQQQS LQLGLNSMEE QDGGPNTIAL ATELQNMKHQ FSVLKGQAEF QMKRIWGEKE
KNALEDAMNN LQKQLEALEP LNIKVENQIK KCEVRNKMKE IIQWVKNLLG ELTPTISLFP
DDILSQIRKC KVKHDGILDK QQAVESLVEE VKKNIPNLTA DESNDLNHLL QDLQNQYQML
ILKSTERSQQ LEFKLEERNR FIAVIEKLQL SLQENEAQII PKMKTASTEP ELEQEHVTLT
SSQEALQEAE SVISARLQEQ ADVCKDLCVF ERLFLGDQLK NLKTRANRTQ RVIQNKCNEV
ERKIHFYREY HEKASVLQKE VDHIQHSELP LNQEIHEDVK EELYSLKDLI TGVQSRILQV
LKLKEVFDSL GLNWDSSQLD QLQAQVFEKE KELEEKIKQL DTLVAERDKY QASLSKLRAM
DLQIKTRAEA LLEMPHASPA SSLLSAQILQ RRIEKAMCLC QEMIKNLNQS KTFDDSFKEK
EILQIKLCVE ENDKLHKILQ DKVLESQPKE MGEKNFQDKL ESSLHVLNQI KSQLEQPLLI
NLDIEHIQNE KENCEAFQEQ VQVEMDSLKA MTLIEKQREE NSLETCEVET KLHDIEDLHM
QLNTSIDLRT DVLNDAYESM TRYNEAVTRA MGIFTACEAI VASHKVDLDN PEEPLEMSYW
KQEELQSTMA DIQDLTEKLG TISSPEAKQQ LQYTLQELVS KDSAMREAAK VKKSEIERCL
ENYKCYRKIK EKVCTNLSQM ETVLGQSMFP LPVSYKEALE RVEQSKTLGS NLLSTKEEFM
ELRQVLRHLR PMCTDSDDRC LLRTTLALWD KWLSLLEAAK EWELWCEELK QEWKFVSEEI
EREAIILDNL QEDLPEISKT KEAATTEELC DLQDCLCRYQ ENVEKQQLLL TLLFQRMRSL
QNAPKSLEAV ETIPTFQEIT SMQKRCNKLF QKAQENKELM ENEIQERHSF TKEIISLKNL
FQQTAASFQN MELQDHPERA DQFEELQSIF KKGKLTFENI MEKLRMKYSE MYTLVPAEIE
SQVEECRKAL EDIDEKISNE VLKSSPSYTM SRKIDEINNG LHNVEKMLQQ KSKNIEKAQE
IQKKMWDELD LWHSKLNELD SEVQDIVEQD PGQAQEWMDN LMIPFQQYQQ VSQRAESRTS
QVNKATVKME EYHDLLKSTE AWIENTNRLL ANPADYDSSK ALSQHASTLQ MALEDSEQKH
NILHSIFTDL EDLSIIFETD DLAQSVQELN HQVAALQQKI MESLPQIQRV ADDVVAIETE
VKSIEKKVSK IKAILLSKEI FDFSPEEHLK HGEVILENIR PMKKAIAELV SYQVELRWTQ
MGMKPLPVFQ RTNQLLQDIK LLENVTQEQN ELLKIAIKQT DECNEEIENL KQILNNYSTE
FSHECISPDQ ATNLPQVQGN IKVMEKQILS LTQKREDLLV DLKAAILNLH QHLQQDQQES
EQEMPSTGAS EEEGVAERDV SEWKLNKTSS MSFLPALKEE VEESSLKSEN GDKKAKPSFG
SLLWKHDGDM EEDRASSSSG TIIQEAVEKI STCDNPMAQI LAPDSLSPNQ TEEGATPTVK
AAALGFSNEQ RAFETTVEKA RSKPAEILHA CKTKVAELEL WLRQANVAFE PETLNADMQQ
LVEQQLVGCQ AMLTDIEHKV ASLLESCKDL GPGDSGATQQ EAEALSLKLK TVKCNLEKVQ
IMLQEKYSED QHSTTLKKPL EHPNDLQADN LSEFESIVIE RPQFGKQKDF QQQQVLELKP
MEQKDFIKFI DFNAKKMWPP YCQPDENVTQ KSTESMRASS LENCAPGSGL STQDESGDKW
QYLHHELSSK IGLPPPKQVQ PQVATNMSVL PNVSMYNFRY PTTEELKTYT TQLEDLRQEA
NNLQTQENMT EDTYINLNTK LFELFLTLSQ CLSSVEEMLQ TPGLSREDVC AQQAHYETLA
LELKKLYLAM GDKKDDLVKA MTWPERNTNL FLECFNNLQV YLEHTQVAAA SRSKSLKAGL
DYNRSYQNEI KRLYDQLTKN KTSLQQSLNE ISGQSIDEQL QKADAYTVEL QNSESRVAKL
RDEGERLHLP YALLQEVYKL EDVLDSMWGI LRARYTELSS PCITERQENA LLTGMVELVD
IGKEKLAHDH LQQAKSKVAL QAQIQNHKLF FQKLVADMLL IQTYSNKMLP SLLQKKETFW
AEQIKEIKSL EERSHQCGIK LQSLLQKWEE FDENYTSLEK DLEILVSTLP SVSLVEETEE
RLVERISFYQ QIKRNIDEKH ARLYQTLNEG KQLVASVSCP ELEGQITKLE EQWLALNKKI
DHELHRLQTL LKHLLSYNRD SDQLTKWLES SQQTLNYWKE QSLNVSQDLD TIRSNINNFF
EFSKELDDKS SLKTAVVSTG SQLLHLKEAD TATLRASLAQ FEQKWTMLIT QLPDIQEKLH
QLQMEKLPSR KAIMEMISWM NNVEHQTSDE DPECSLSSAS QVKSLLQKYK EFRMEMDYKQ
WIVDFVNQSL LQLSTCDVES KRYERTEFAE HLGEMNRQWH RVHGTLNRKI QHLEQLLESI
SENENKIQIL NNWMEAQEER LKTLQKPGSV ISVQKMLLDC QDIENQLAIK SKALDELRQS
YLTSESGTMP LLEDTASRID GLFQKRSSVI NQVNELKTSM QSVLQEWKVY DKLYEEVTMM
TIRFWYCMDH SKPVVLSLEA LRCQVQNLQS LQDEAESSEG SWEKLQEVIR KLKDHCPSVA
EIIEEKCQDT HARWTQVNQD IAEQLQKAQS LLQLWKAYNS AHTEAAARLE QQEAKYQQLE
NINMSGNNLA EILTSALQDV KELQQDVQKT KEAFLQNSTL LDRLTQLTES STHVPLSTQL
HSLQRASYLE KMLLVKANEF EFVLSQFKDF GDRLESLKGL IMREEENLDK LYHQEKEENP
DLFLKHVLAL TAQSPDVEHL NAVSLKLPLS DIAVKTLQNM NRQWIRATAT ALERCSELQG
IGLNEKFLCC CEKWVQLLEK IEETLKVNIG NSLPALLEQQ KTYGMLEAEV SINQTIADSY
VTQCLQLLDT TEGEKRREFI SKFTKLKDQW QRAAQGIQQR KSDIDGLVGQ WQYFTTSVDN
LLRFLTDTCH LLSAVKSQDC YGLHQTRRLI QELKSKEIHF QRQQTTFELT LEAGEKLLNT
ANLETQDLIE KKISQIRDDW ENTKLQVGEM IQQLQSNVET WGQCEKKIKE LKSRLQVIKA
QSRDPLPELH EDLHREKELI KELEKSLGNW TQNLKELHTM KTDLTQHILV EDVLVLEEQT
EHLHRQWEDL CLRVAIRKQE IEDRLNSWIV FNEKNKELCA WLVQMENKVL QTADISIEEM
IEKLQKDCME EINLFSENKL QLKQMGDQLI KASNKTRAAE IEDKLSKVND RWQHLFDVIG
SRVKKLKETF AFIQQLDKNM SNLRTWLARI ESELSKPVVY DVCDDQEIQK RLAEQQDLQR
DIEQHSAGVE SVFNICDVLL HDSDACANET ECDSIQQTTR SLDRRWRNIC AMSMERRMKI
EETWRLWQKF LDDYSRFEDW LKSAERTAAY PNSSEVLYTN AKEELKRFEA FQRQIHERLT
QLELINKQYR RLARENRTDT ASKLKHMVHE GNQRWDNLQK RVTAVLRRLR HFTNQREEFE
GTRESILVWL TEMDLQLTNV EHFSESDADD KMRQLNGFQQ EITLNTNKID QLIVFGEQLI
QKSEPLDAVL IEDELEELHR YCQEVFGRVS RFHRRLTSHT PGLEEEKEAS ENETDMEDPR
EIQADSWRKR RESEEPSSPQ SLCHLVPPAP GPERSGCETP VSVDSIPLEW DHTGDVGGSS
SHEDDEEGPY YSALSDVEIP ENPEAYLKMT TKTLKASSGK SISEDHSWHV PDSPSCPKHH
YKQMGGDRNV QTIPSDSSTP YKPAYVKLLL SPGTDGGKEG SRVLNGNSRQ ENEGLAALTR
QQSGAFDRWE LIQAQELHNQ LRIKQNLQQL NSEISDITTW LKETEAELET LKAAEPPSDI
REMQLRVKRL KEILKAFDTY KAVVVSVNTS SQEFLQTESA ESNELQGRVG QLGLRWDAAQ
GAVESWREGL RQSLMQCQDF HQLSQDLLLW LASAESRRQK AHVTDPEADP QVLLECQGEL
MQLEKELVER QPPVNTLREI SDSLLTKGHG EDYIETEEKV HVIEKKLNQL LEQVSQDLMS
LQGSQNPDPS LPSLDEVDSG DQPPGASVPA PQAQQCGAER MTKDQNKMDS RVPGPAGPGS
SRPQRSFVSR VIRAALPLQL LLLLLLLLAC LLPSSEEDYS CTQANNFARS FYPMLRYTNG
PPPT
//
