ID A0A3Q9G0B1_9ACTO Unreviewed; 444 AA.
AC A0A3Q9G0B1;
DT 10-APR-2019, integrated into UniProtKB/TrEMBL.
DT 10-APR-2019, sequence version 1.
DT 27-MAR-2024, entry version 19.
DE SubName: Full=6-phospho-beta-glucosidase {ECO:0000313|EMBL:AZQ76065.1};
GN ORFNames=EJ997_00735 {ECO:0000313|EMBL:AZQ76065.1};
OS Flaviflexus ciconiae.
OC Bacteria; Actinomycetota; Actinomycetes; Actinomycetales; Actinomycetaceae;
OC Flaviflexus.
OX NCBI_TaxID=2496867 {ECO:0000313|EMBL:AZQ76065.1, ECO:0000313|Proteomes:UP000280344};
RN [1] {ECO:0000313|EMBL:AZQ76065.1, ECO:0000313|Proteomes:UP000280344}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=H23T48 {ECO:0000313|EMBL:AZQ76065.1,
RC ECO:0000313|Proteomes:UP000280344};
RA Bae J.-W., Lee J.-Y.;
RT "Complete genome sequence of Flaviflexus sp. H23T48.";
RL Submitted (DEC-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=NAD(+); Xref=ChEBI:CHEBI:57540;
CC Evidence={ECO:0000256|RuleBase:RU361152};
CC Note=Binds 1 NAD(+) per subunit. {ECO:0000256|RuleBase:RU361152};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 4 family.
CC {ECO:0000256|ARBA:ARBA00010141, ECO:0000256|RuleBase:RU361152}.
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DR EMBL; CP034593; AZQ76065.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3Q9G0B1; -.
DR KEGG; flh:EJ997_00735; -.
DR OrthoDB; 9767022at2; -.
DR Proteomes; UP000280344; Chromosome.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 3.90.110.10; Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR001088; Glyco_hydro_4.
DR InterPro; IPR022616; Glyco_hydro_4_C.
DR InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR PANTHER; PTHR32092:SF5; 6-PHOSPHO-BETA-GLUCOSIDASE; 1.
DR PANTHER; PTHR32092; 6-PHOSPHO-BETA-GLUCOSIDASE-RELATED; 1.
DR Pfam; PF02056; Glyco_hydro_4; 1.
DR Pfam; PF11975; Glyco_hydro_4C; 1.
DR PRINTS; PR00732; GLHYDRLASE4.
DR SUPFAM; SSF56327; LDH C-terminal domain-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 3: Inferred from homology;
KW Cobalt {ECO:0000256|PIRSR:PIRSR601088-3};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361152};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361152};
KW Iron {ECO:0000256|PIRSR:PIRSR601088-3};
KW Manganese {ECO:0000256|PIRSR:PIRSR601088-3};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR601088-3};
KW NAD {ECO:0000256|RuleBase:RU361152};
KW Nickel {ECO:0000256|PIRSR:PIRSR601088-3};
KW Reference proteome {ECO:0000313|Proteomes:UP000280344}.
FT DOMAIN 187..419
FT /note="Glycosyl hydrolase family 4 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF11975"
FT ACT_SITE 163
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR601088-1"
FT ACT_SITE 241
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR601088-1"
FT BINDING 88
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR601088-2"
FT BINDING 142
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR601088-2"
FT BINDING 162
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000256|PIRSR:PIRSR601088-3"
FT BINDING 192
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000256|PIRSR:PIRSR601088-3"
FT SITE 104
FT /note="Increases basicity of active site Tyr"
FT /evidence="ECO:0000256|PIRSR:PIRSR601088-4"
SQ SEQUENCE 444 AA; 48179 MW; 0BB2B338EE55E819 CRC64;
MKLLLVGGGG FRVPQILSVL ANPNLPLNLT EVCLYDVADD RLAVMKDVVN QLGYKNLPTV
TTTTDMVDAV KGADFIFSAM RIGGTCGRVI DEREALTRGI LGQETVGAGG YAYAFRTIPE
AMRLAETVKQ HAPDAWIINF TNPAGIITQA MRTVHPRVTG ICDTPIGLVR RASLIAGFDE
KDITYDYIGL NHLGWLRSLN AGGDDVLPSI LADDNQLVRL EEARTVGFDW VRALGMIPNE
YLYYYYFNRE SVEKISAGQT RGEFLQAQQG SFYDAAQADP ARAGDLWTQA HNEREATYMA
ESRDEGESRR EEDMGGGYEH VALELMTALA TGTPARMILG VANNDGDSLI IPQLRDDAVV
EVPCVVDSNG ITPINQGPLS GPELGLVTSV KACEELVIDA AISGDKTLAW RALAAHPLVD
SVGVAKDVLD AYIDKNPQIA KTFE
//
