ID   A0A3Q9IZ64_9MICO        Unreviewed;       824 AA.
AC   A0A3Q9IZ64;
DT   10-APR-2019, integrated into UniProtKB/TrEMBL.
DT   10-APR-2019, sequence version 1.
DT   27-MAR-2024, entry version 21.
DE   RecName: Full=DNA topoisomerase (ATP-hydrolyzing) {ECO:0000256|ARBA:ARBA00012895};
DE            EC=5.6.2.2 {ECO:0000256|ARBA:ARBA00012895};
GN   Name=gyrA_2 {ECO:0000313|EMBL:AZS36828.1};
GN   ORFNames=CVS47_01441 {ECO:0000313|EMBL:AZS36828.1};
OS   Microbacterium lemovicicum.
OC   Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC   Microbacterium.
OX   NCBI_TaxID=1072463 {ECO:0000313|EMBL:AZS36828.1, ECO:0000313|Proteomes:UP000276888};
RN   [1] {ECO:0000313|EMBL:AZS36828.1, ECO:0000313|Proteomes:UP000276888}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Viu22 {ECO:0000313|EMBL:AZS36828.1,
RC   ECO:0000313|Proteomes:UP000276888};
RA   ORTET P.;
RT   "Microbacterium lemovicicum sp. nov., a bacterium isolated from a natural
RT   uranium-rich soil.";
RL   Submitted (AUG-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP-dependent breakage, passage and rejoining of double-
CC         stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000256|ARBA:ARBA00000185};
CC   -!- SIMILARITY: Belongs to the type II topoisomerase GyrA/ParC subunit
CC       family. {ECO:0000256|ARBA:ARBA00008263}.
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DR   EMBL; CP031423; AZS36828.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A3Q9IZ64; -.
DR   KEGG; mlv:CVS47_01441; -.
DR   OrthoDB; 9806486at2; -.
DR   Proteomes; UP000276888; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0034335; F:DNA negative supercoiling activity; IEA:UniProt.
DR   GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR   CDD; cd00187; TOP4c; 1.
DR   Gene3D; 3.30.1360.40; -; 1.
DR   Gene3D; 2.120.10.90; DNA gyrase/topoisomerase IV, subunit A, C-terminal; 1.
DR   Gene3D; 3.90.199.10; Topoisomerase II, domain 5; 1.
DR   Gene3D; 1.10.268.10; Topoisomerase, domain 3; 1.
DR   InterPro; IPR006691; GyrA/parC_rep.
DR   InterPro; IPR035516; Gyrase/topoIV_suA_C.
DR   InterPro; IPR013760; Topo_IIA-like_dom_sf.
DR   InterPro; IPR013758; Topo_IIA_A/C_ab.
DR   InterPro; IPR013757; Topo_IIA_A_a_sf.
DR   InterPro; IPR002205; Topo_IIA_dom_A.
DR   PANTHER; PTHR43493:SF5; DNA GYRASE SUBUNIT A, CHLOROPLASTIC_MITOCHONDRIAL; 1.
DR   PANTHER; PTHR43493; DNA GYRASE/TOPOISOMERASE SUBUNIT A; 1.
DR   Pfam; PF03989; DNA_gyraseA_C; 3.
DR   Pfam; PF00521; DNA_topoisoIV; 1.
DR   SMART; SM00434; TOP4c; 1.
DR   SUPFAM; SSF101904; GyrA/ParC C-terminal domain-like; 1.
DR   SUPFAM; SSF56719; Type II DNA topoisomerase; 1.
PE   3: Inferred from homology;
KW   DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW   Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000313|EMBL:AZS36828.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000276888};
KW   Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT   DOMAIN          20..473
FT                   /note="DNA topoisomerase type IIA"
FT                   /evidence="ECO:0000259|SMART:SM00434"
SQ   SEQUENCE   824 AA;  87501 MW;  21E5E89CBA950C6A CRC64;
     MRATPPSPAQ DRAPERIEDV DVSVEMQGSF LEYAYSVIYS RALPDARDGL KPVQRRILYQ
     MADMGLRPDR GHVKSARVVG EVMGKLHPHG DAPIYDALVR LAQAFSLRVP LVDGHGNFGS
     LDDGPAAPRY TEARLAAAAL ALTQDLDEDV VDFIPNYDGQ FQQPEVLPAA FPNLLVNGTT
     GIAVGMATNM APHNLIEVVA AAVHLLENPE ATVEELMEFV PGPDLPSGGI IVGLDGIKDA
     YAKGRGSFRT RAKVSVEPLG PRRTGLVVTE LPYLVGPERI IEKIKDAVTS KKLTGIADVT
     DLTDRHHGLR LVIGIKTGFD PLAVLEQLYR LTPLEDSFSI NNVALVGGQP QTLGLRELLR
     VYLDHRVSVV TRRSRFRLQR RRDRLHLVEG LLVAILDIDE VIQVIRTSDD SEQARARLMD
     VFDLSVLQAE YILELRLRRL TKFSRVELEA ERDDLKAQIA ALEELLGSDV LLRAQVAREL
     DAAAEAHGTP RRTVLLNGGP VAARPSRAAA AAAADLQIAD APCRVYLSAT GRMLRAEVSP
     DAPGGGIVPP VRRSKHDAIR SAVDATTRGD VGAVTSAGRL VRFSPVDLPS VPGNSVQPAA
     GARADQYLGL SSGEHVVALV ALTGEPPIAL GTAQGVVKRV AATELPLNRH DIDVIALKPG
     DRVVGAAPAP DGAELVFVTS DAQLLRFDAS SVRPQGRAAG GMAGVRLADG ESAVFFGAVE
     AAASDVVVVT VAGSRQALAG ADAASVKVSS FEEFPPKGRA TGGVRAQRFL RGEDTLALAW
     VGLGPARAVG ADGAVRPLPE PVAKRDASGQ PLDGLVGAIG EPVR
//