ID A0A3R7D6Q8_CLOSI Unreviewed; 621 AA.
AC A0A3R7D6Q8;
DT 10-APR-2019, integrated into UniProtKB/TrEMBL.
DT 10-APR-2019, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=tRNA (guanine(26)-N(2))-dimethyltransferase {ECO:0000256|ARBA:ARBA00039099, ECO:0000256|PROSITE-ProRule:PRU00958};
DE EC=2.1.1.216 {ECO:0000256|ARBA:ARBA00039099, ECO:0000256|PROSITE-ProRule:PRU00958};
GN ORFNames=CSKR_111848 {ECO:0000313|EMBL:KAG5445295.1};
OS Clonorchis sinensis (Chinese liver fluke).
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Platyhelminthes; Trematoda;
OC Digenea; Opisthorchiida; Opisthorchiata; Opisthorchiidae; Clonorchis.
OX NCBI_TaxID=79923 {ECO:0000313|EMBL:KAG5445295.1, ECO:0000313|Proteomes:UP000286415};
RN [1] {ECO:0000313|EMBL:KAG5445295.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Cs-k2 {ECO:0000313|EMBL:KAG5445295.1};
RX PubMed=29454982;
RA Wang D., Korhonen P.K., Gasser R.B., Young N.D.;
RT "Improved genomic resources and new bioinformatic workflow for the
RT carcinogenic parasite Clonorchis sinensis: Biotechnological implications.";
RL Biotechnol. Adv. 36:894-904(2018).
RN [2] {ECO:0000313|EMBL:KAG5445295.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Cs-k2 {ECO:0000313|EMBL:KAG5445295.1};
RX PubMed=33677057;
RA Young N.D., Stroehlein A.J., Kinkar L., Wang T., Sohn W.M., Chang B.C.H.,
RA Kaur P., Weisz D., Dudchenko O., Aiden E.L., Korhonen P.K., Gasser R.B.;
RT "High-quality reference genome for Clonorchis sinensis.";
RL Genomics 0:0-0(2021).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=guanosine(26) in tRNA + 2 S-adenosyl-L-methionine = 2 H(+) +
CC N(2)-dimethylguanosine(26) in tRNA + 2 S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:43140, Rhea:RHEA-COMP:10359, Rhea:RHEA-COMP:10360,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:74269, ChEBI:CHEBI:74513; EC=2.1.1.216;
CC Evidence={ECO:0000256|PROSITE-ProRule:PRU00958};
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. Trm1 family. {ECO:0000256|PROSITE-ProRule:PRU00958}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAG5445295.1}.
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DR EMBL; NIRI02000056; KAG5445295.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3R7D6Q8; -.
DR STRING; 79923.A0A3R7D6Q8; -.
DR InParanoid; A0A3R7D6Q8; -.
DR Proteomes; UP000286415; Chromosome 2.
DR GO; GO:0160102; F:tRNA (guanine(10)-N2)-methyltransferase activity; IEA:InterPro.
DR GO; GO:0160104; F:tRNA (guanine(26)-N2)-dimethyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0006400; P:tRNA modification; IEA:UniProt.
DR Gene3D; 3.30.56.70; N2,N2-dimethylguanosine tRNA methyltransferase, C-terminal domain; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR002905; Trm1.
DR InterPro; IPR042296; tRNA_met_Trm1_C.
DR PANTHER; PTHR10631; N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE; 1.
DR PANTHER; PTHR10631:SF3; TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE; 1.
DR Pfam; PF02005; TRM; 2.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 2.
DR PROSITE; PS51626; SAM_MT_TRM1; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU00958};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW ProRule:PRU00958};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU00958};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU00958}; tRNA processing {ECO:0000256|PROSITE-ProRule:PRU00958};
KW tRNA-binding {ECO:0000256|ARBA:ARBA00022555, ECO:0000256|PROSITE-
KW ProRule:PRU00958}.
SQ SEQUENCE 621 AA; 68053 MW; 3B324BFCB009A995 CRC64;
MVESPNASRP VIGHVEEGRA RIILPEGVFY NRVQEFNRDI TVAMLQQYQL LHQSEWIERK
QRQKKRGVEA TDATYDGLRI LEALSATGIR SVRFALEVPK ISKIVANDLD AKAVELIRNN
VELNGVGKHI DVTCMDAVEL MHKCKTFTER FNVVDIDPYG TASPFLDSAV QCLHDGGLLC
VTSTDAAVLC GSTPGTSMGK YGGLAIKNSA PHETSLRILL HAIQSAASRF GCVIEPLLSL
SVDFYVRVFV RIWASPGSVK AIAAKHAICF SCTGCTAYHV QPLGQALPSN KRLAPARGPP
VGPLCAECGS HFNVWGPIWC GPLHSRSFLS GFLTTLGCPP KVPITPVAQD GDDCNTALPQ
SDKIDRIPRD PSNNIYPSGA ELGLTMPEEF SCAPDLTDPD LSGKASSRFG TFKRIVGMCT
MAFEELPDVI LYHRIDQLTS VLGMQVPALL ELHSPFLNAG YRISCSHTET NTFKTDAPMS
FIWDVLLTIH QKFQLSEKAH KSPTSSATSD ESDSAELKTA SGNAESVQPK QSGRRTNRQH
RRKRLESLDP EQAAELASLR AHRGQIRARL LSRSINSIVD LTIHPDANPP SRTDGLVRYQ
PNPEKFWGPK ARPKMQKTDS G
//
