ID   A0A3R8SZP2_9BURK        Unreviewed;      1180 AA.
AC   A0A3R8SZP2;
DT   10-APR-2019, integrated into UniProtKB/TrEMBL.
DT   10-APR-2019, sequence version 1.
DT   27-MAR-2024, entry version 16.
DE   RecName: Full=DNA polymerase III subunit alpha {ECO:0000256|ARBA:ARBA00019114};
DE            EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN   ORFNames=EIP75_18460 {ECO:0000313|EMBL:RRS02760.1};
OS   Aquabacterium soli.
OC   Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC   Aquabacterium.
OX   NCBI_TaxID=2493092 {ECO:0000313|EMBL:RRS02760.1, ECO:0000313|Proteomes:UP000269265};
RN   [1] {ECO:0000313|EMBL:RRS02760.1, ECO:0000313|Proteomes:UP000269265}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SJQ9 {ECO:0000313|EMBL:RRS02760.1,
RC   ECO:0000313|Proteomes:UP000269265};
RA   Sun L., Gao X., Chen W., Huang K.;
RT   "The whole draft genome of Aquabacterium sp. SJQ9.";
RL   Submitted (DEC-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC         Evidence={ECO:0000256|ARBA:ARBA00024632};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:RRS02760.1}.
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DR   EMBL; RSED01000018; RRS02760.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A3R8SZP2; -.
DR   OrthoDB; 9803237at2; -.
DR   Proteomes; UP000269265; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   CDD; cd04485; DnaE_OBF; 1.
DR   CDD; cd07433; PHP_PolIIIA_DnaE1; 1.
DR   Gene3D; 1.10.150.870; -; 1.
DR   Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1.
DR   Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR004805; DnaE2/DnaE/PolC.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004365; NA-bd_OB_tRNA.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR049821; PolIIIA_DnaE1_PHP.
DR   NCBIfam; TIGR00594; polc; 1.
DR   PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR   PANTHER; PTHR32294:SF0; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   Pfam; PF01336; tRNA_anti-codon; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; PHP domain-like; 1.
PE   4: Predicted;
KW   DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW   DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW   Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW   ECO:0000313|EMBL:RRS02760.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000269265};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:RRS02760.1}.
FT   DOMAIN          4..71
FT                   /note="Polymerase/histidinol phosphatase N-terminal"
FT                   /evidence="ECO:0000259|SMART:SM00481"
SQ   SEQUENCE   1180 AA;  129603 MW;  4A2374733DFFA31E CRC64;
     MAFVHLRSHT EFSVVDGTVR IDDLVKAAAK DGQPAVAVTD LSNLFGAIKL YSSARKKGVQ
     PLIGADVWLE ADEPGRPVSR LLLLIQNRKG YLHLSELLGE AWTAPGQRTH ASVFWESLAQ
     RSEGLIALAG AEMGCVGQAL MNGDAGRAEA LARRLAGIFP GRFYIELQRA GHPTNEAHIR
     AAVPLAAKIG LPVVATHPIQ FSTPDDFEAH EARTCIAEGE TLGNPRRIKR FTREQYFKTQ
     AQMEALFADI PSAVANTVAI ARRCALTLVL GKPQLPDFPT PILQDGPDAG KPMPMADYFR
     QLSFEGLEER LAHLFPDRAV RDARRPEYVS RLEFEINTIL KMGFPGYFLI VADFINWAKN
     NGCPVGPGRG SGAGSLVAYA LKITDLDPLE YNLLFERFLN PERVSMPDFD IDFCQGNRDR
     VIDYVKDKYG RSAVSQIATF GTMAAKAALR DVGRVLGMGY GHVDSIAKLI PAPPGKSVTL
     AKVPENPDPG VIYARKEAPE LEQREAAEEE VASLLALAER VEGMVRNIGM HAGGVLIAPG
     KITDFCPLYM QPGSDSAVSQ YDKDDVEAIG LVKFDFLGLA TLTILETAKE FIMARHPDQK
     DFDFAKIKLD DAATYRLLSE GKTVAVFQLE SRGMQGMLKD AKPSVFEDII ALVSLYRPGP
     MDLIPSFCAR KHGKEEVEYL HPLMAEVLEE TYGIMVYQEQ VMQVAQRVGG YSLGGADMLR
     RAMGKKKLEE MVAHRATFAE GAAKNDIKPD LANEIFDLME KFAGYGFNKS HAAAYALLAY
     HTAWLKVHYT AEFFAGNMSV SSDDTDKLKI FHDDATQNFG ITFLPPNVNL GAWRFEPVDD
     KTVCYALGAV KGTGQGAIEA IVRERTANGP FKSFFEFCAR VDRKSVNKRV VEALIKGGAF
     DALEPHRAGL LASVPLAFDY ADSLAANADQ GGLFDFGDSH AASTNEPELV HAEPWSLKEQ
     LSLEKTAIGF YLSGHLFDEA EKEVRRFAKR VGDLQDARDP QMTAGIVTEL RVINGVRGRV
     ALFKLDDKSD VIEAVANEEL LDANRDLLKD DELLVIQGKV QPDRFSGGLR LNVQQIWSLG
     AARCRFARFL RVKVKDQQCP VADVLREFPA RRVPAPQPDL PETVQGLPIR VVVERVAADV
     SARAELELGD GARFFPSDQA LVRIKELMPA GLPEIVYGEG
//