ID A0A3R9FQ57_9GAMM Unreviewed; 718 AA.
AC A0A3R9FQ57;
DT 10-APR-2019, integrated into UniProtKB/TrEMBL.
DT 10-APR-2019, sequence version 1.
DT 24-JAN-2024, entry version 18.
DE SubName: Full=Fatty acid oxidation complex subunit alpha FadJ {ECO:0000313|EMBL:RRZ94730.1};
DE EC=1.1.1.35 {ECO:0000313|EMBL:RRZ94730.1};
DE EC=4.2.1.17 {ECO:0000313|EMBL:RRZ94730.1};
DE EC=5.1.2.3 {ECO:0000313|EMBL:RRZ94730.1};
GN Name=fadJ {ECO:0000313|EMBL:RRZ94730.1};
GN ORFNames=EGK14_06770 {ECO:0000313|EMBL:RRZ94730.1};
OS Erwinia sp. 198.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Erwiniaceae; Erwinia.
OX NCBI_TaxID=2022746 {ECO:0000313|EMBL:RRZ94730.1, ECO:0000313|Proteomes:UP000273595};
RN [1] {ECO:0000313|EMBL:RRZ94730.1, ECO:0000313|Proteomes:UP000273595}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=198 {ECO:0000313|EMBL:RRZ94730.1,
RC ECO:0000313|Proteomes:UP000273595};
RA D'Souza A.W., Potter R.F., Wallace M., Shupe A., Patel S., Sun S., Gul D.,
RA Kwon J.H., Andleeb S., Burnham C.-A.D., Dantas G.;
RT "Transmission dynamics of multidrug resistant bacteria on intensive care
RT unit surfaces.";
RL Submitted (OCT-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+) +
CC NADH; Xref=Rhea:RHEA:22432, ChEBI:CHEBI:15378, ChEBI:CHEBI:57318,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:90726; EC=1.1.1.35;
CC Evidence={ECO:0000256|ARBA:ARBA00023693};
CC -!- PATHWAY: Lipid metabolism; fatty acid beta-oxidation.
CC {ECO:0000256|ARBA:ARBA00005005}.
CC -!- SIMILARITY: In the central section; belongs to the 3-hydroxyacyl-CoA
CC dehydrogenase family. {ECO:0000256|ARBA:ARBA00007005}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RRZ94730.1}.
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DR EMBL; RHUM01000003; RRZ94730.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3R9FQ57; -.
DR OrthoDB; 5389341at2; -.
DR UniPathway; UPA00659; -.
DR Proteomes; UP000273595; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0008692; F:3-hydroxybutyryl-CoA epimerase activity; IEA:InterPro.
DR GO; GO:0004300; F:enoyl-CoA hydratase activity; IEA:InterPro.
DR GO; GO:0070403; F:NAD+ binding; IEA:InterPro.
DR GO; GO:0006635; P:fatty acid beta-oxidation; IEA:UniProtKB-UniPathway.
DR CDD; cd06558; crotonase-like; 1.
DR Gene3D; 1.10.1040.50; -; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR006176; 3-OHacyl-CoA_DH_NAD-bd.
DR InterPro; IPR006108; 3HC_DH_C.
DR InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR InterPro; IPR012802; FadJ.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR NCBIfam; TIGR02440; FadJ; 1.
DR PANTHER; PTHR43612; TRIFUNCTIONAL ENZYME SUBUNIT ALPHA; 1.
DR PANTHER; PTHR43612:SF3; TRIFUNCTIONAL ENZYME SUBUNIT ALPHA, MITOCHONDRIAL; 1.
DR Pfam; PF00725; 3HCDH; 1.
DR Pfam; PF02737; 3HCDH_N; 1.
DR Pfam; PF00378; ECH_1; 1.
DR SUPFAM; SSF48179; 6-phosphogluconate dehydrogenase C-terminal domain-like; 2.
DR SUPFAM; SSF52096; ClpP/crotonase; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Fatty acid metabolism {ECO:0000256|ARBA:ARBA00022832};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000313|EMBL:RRZ94730.1};
KW Lipid degradation {ECO:0000256|ARBA:ARBA00022963};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00022832};
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000313|EMBL:RRZ94730.1};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW NAD {ECO:0000256|ARBA:ARBA00023027};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000313|EMBL:RRZ94730.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000273595}.
FT DOMAIN 310..489
FT /note="3-hydroxyacyl-CoA dehydrogenase NAD binding"
FT /evidence="ECO:0000259|Pfam:PF02737"
FT DOMAIN 492..585
FT /note="3-hydroxyacyl-CoA dehydrogenase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF00725"
SQ SEQUENCE 718 AA; 77672 MW; A3AD56E9450519D8 CRC64;
MMTASAFTFS LRADNVGIIT FDVPGEKLNT LKAEFVLQIR EAIAQARRQP ELAGLVFISG
KPDSFIAGAD ITMIAACRSA EEAAALARQG QKVMAEIAAL PFPAVAAIHG ACLGGGLELA
LACHQRVCTL DEKTQLGLPE VQLGLLPGSG GTQRLPRLIG ASHALDLMLT GKTLRAKQAL
KLGLVEEAVP QTILLETAVD MVLRGRQPRR VLPLSERMLN APMGRALLFK MATRRAEAKT
RGNYPATRRI LSVVRTGLQQ GRSAGYAAEA KAFGELAMSP ESIALRGLFF ATTEIKKESG
AQAAPEEVKS VAVLGGGLMG GGIAYVTACR GNLPVRIKDV RPEGITHALK YSWDLLEKKI
ARRQLTPGQR QRQMALISGG LDYQGFRRRD LVIEAVFEDL FLKQKMVAEV EANTSPGTIF
ASNTSSLPIS DIAAHARRPE HIIGMHYFSP VDKMPLVEVI PHAQTSEQTL ATTVKLAKLQ
GKTPIVVADS AGFYVNRILT PCINEALRCL LEGEPIENID EALADFGFPV GPIQLLDEVG
IDTGAHILPV LAEAWGERFQ APPELDAVLN DNRKGRKNGR GFYRYGKKEG GKKRPDATIY
KLLNVHPKAH LSKELIARRC VMMLLNEAAR AFDENVVRCA RDGDIGAVFG IGFPPFTGGP
FRYMDALGAG QIVAQLEQLS RSCGARFTPC ERLRKMAQTG ETFYKTTGKA NIQPVSTG
//
