ID A0A3R9NAX1_9BACI Unreviewed; 363 AA.
AC A0A3R9NAX1;
DT 10-APR-2019, integrated into UniProtKB/TrEMBL.
DT 10-APR-2019, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE SubName: Full=Radical SAM protein {ECO:0000313|EMBL:RSK29249.1};
GN ORFNames=EJF36_04595 {ECO:0000313|EMBL:RSK29249.1};
OS Bacillus sp. HMF5848.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=2495421 {ECO:0000313|EMBL:RSK29249.1, ECO:0000313|Proteomes:UP000274811};
RN [1] {ECO:0000313|EMBL:RSK29249.1, ECO:0000313|Proteomes:UP000274811}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HMF5848 {ECO:0000313|EMBL:RSK29249.1,
RC ECO:0000313|Proteomes:UP000274811};
RA Kang H., Kang J., Cha I., Kim H., Joh K.;
RT "Bacillus sp. HMF5848 Genome sequencing and assembly.";
RL Submitted (DEC-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RSK29249.1}.
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DR EMBL; RWIV01000001; RSK29249.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3R9NAX1; -.
DR OrthoDB; 9782387at2; -.
DR Proteomes; UP000274811; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd21123; SPASM_MftC-like; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR023885; 4Fe4S-binding_SPASM_dom.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR034391; Cmo-like_SPASM_containing.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR017200; PqqE-like.
DR InterPro; IPR007197; rSAM.
DR NCBIfam; TIGR04085; rSAM_more_4Fe4S; 1.
DR PANTHER; PTHR11228; RADICAL SAM DOMAIN PROTEIN; 1.
DR PANTHER; PTHR11228:SF34; TUNGSTEN-CONTAINING ALDEHYDE FERREDOXIN OXIDOREDUCTASE COFACTOR MODIFYING PROTEIN; 1.
DR Pfam; PF13353; Fer4_12; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR Pfam; PF13186; SPASM; 1.
DR PIRSF; PIRSF037420; PQQ_syn_pqqE; 1.
DR SFLD; SFLDG01387; BtrN-like_SPASM_domain_contain; 1.
DR SFLD; SFLDG01386; main_SPASM_domain-containing; 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SFLD; SFLDG01067; SPASM/twitch_domain_containing; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000274811};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}.
FT DOMAIN 17..237
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 363 AA; 40665 MW; 2CDDABFFAB493F69 CRC64;
MNAVQDLHLK SHKLRQSFSP RLIFWELTEG CNLKCIHCRA TAQPMRSDAE LSTIQAKRVV
DDIASFADPI LILTGGEPLY RPDFFEIAQY AHDKGLTLAM ATNGTLITED IAKKIKDAGI
RRVAISLDGP TADIHDKFRG IDGSFEAALR GAELVKKQGI QVQFNTTITK HNVHAVEDMI
ALAEAREADA LHLFMLVPVG CGVQITESNM LTATEYENVL EWFYERSREV SFEIRATCAP
HYYRIMRQKA KERGERVTRQ THGMNAMTKG CLAGSGVCFI SHKGFVQPCG YLPVQVGNVL
QQPLEQIWNE SPEFAVLRDT NELDGKCGIC EYVNICSGCR ARAYYETGNF MSEEPYCNYQ
PGK
//
