ID A0A3R9PAP6_9BACT Unreviewed; 942 AA.
AC A0A3R9PAP6;
DT 10-APR-2019, integrated into UniProtKB/TrEMBL.
DT 10-APR-2019, sequence version 1.
DT 27-MAR-2024, entry version 19.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=EDE15_2991 {ECO:0000313|EMBL:RSL17456.1};
OS Edaphobacter aggregans.
OC Bacteria; Acidobacteriota; Terriglobia; Terriglobales; Acidobacteriaceae;
OC Edaphobacter.
OX NCBI_TaxID=570835 {ECO:0000313|EMBL:RSL17456.1, ECO:0000313|Proteomes:UP000269669};
RN [1] {ECO:0000313|EMBL:RSL17456.1, ECO:0000313|Proteomes:UP000269669}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=EB153 {ECO:0000313|EMBL:RSL17456.1,
RC ECO:0000313|Proteomes:UP000269669};
RA Deangelis K.;
RT "Sequencing of bacterial isolates from soil warming experiment in Harvard
RT Forest, Massachusetts, USA.";
RL Submitted (DEC-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RSL17456.1}.
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DR EMBL; RSDW01000001; RSL17456.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3R9PAP6; -.
DR Proteomes; UP000269669; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.30.160.800; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF21196; PcrA_UvrD_tudor; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000269669}.
FT DOMAIN 10..295
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 296..703
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 500..533
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 583..616
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 821..854
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 873..903
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 507..524
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 592..615
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 31..38
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 942 AA; 104684 MW; F9239562B2162109 CRC64;
MCSALSELLM NMNPQQQEGI KSVDGPVLLL AGAGSGKTRV ITHRIAYLIQ ERGVPADSIL
AVTFTNKAAK EMEERVNKIL GHTSLAKPML ATFHSFCVRV LRRDIEALRV NGVGLTKTFA
IYDETDQQAV VKSALKRLAI DDKSLKPRVA LGRISWAKNH MIDPQEYFLA STNPMEEKIA
HIFEIYKKEL FKANALDFDD LLLETVRLLK SSAEVRERYN RRYRYVMIDE YQDTNRPQYE
LMKLLAGSHG NICVVGDEDQ SIYSWRGADI KNILDFEKDF PDVKTIRLEQ NYRSTQMILE
GAGAVVAQNT QRKGKNLWTA REGGSLIGYY EAPDGENEAL FIADRVQRYL REAGQQEEQP
RCAVLYRTNS QSRLVEEALR RYQIQYHMVG GFSFYDRAEV KDILSYLKLV QNVHDSIALN
RVINSPARGI GKTTMETLER MALASGMSTW DAMGRAIEDK LLPQRALINL SGFRRLIEDA
RAMLGPGFAE KLSEDVAPLA EEEGDTSFGF GAEDSDDDEV PVETGAGDAA DTSFDTSFNF
GFDFGPTEEL STIAPENARD SDELHGIDAV SFNPFAPVVL KSSAASRRTK ADPPPSTKDD
KGEGKDLEER PAFRKPGDAA TLPELIKFLN DRSGYIRALE EEATPESFSR IENLKELANA
AQDAQERGET LEEFLDHAAL VSDADQYSAE AKVTLMTLHA AKGLEFPLVF LAGMEEGLFP
HSRTLMDPSG MEEERRLCYV GMTRAMDTLV MTRARYRRRY GSDMPEASVA SRFLEEVPSR
LVEDLGSPPA RPQFSGSRYG GMYATPYPKA NRFGAGREEG ERHYSYEDED QSAPRGGGVS
SGARAKSGAR AASGGGSIDN IASFFAGRGQ AAGQKFSRPK VDVPEPTGKT GLKQGSRVRH
PKYGEGIVFR REGDGDDAKI TVQFQQHGVK KLVEKFAQLE RL
//
