ID A0A3S0AGJ4_9FLAO Unreviewed; 1478 AA.
AC A0A3S0AGJ4;
DT 10-APR-2019, integrated into UniProtKB/TrEMBL.
DT 10-APR-2019, sequence version 1.
DT 13-SEP-2023, entry version 21.
DE RecName: Full=DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00012417};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN ORFNames=EHW67_01490 {ECO:0000313|EMBL:RTE55266.1};
OS Arenibacter aquaticus.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Arenibacter.
OX NCBI_TaxID=2489054 {ECO:0000313|EMBL:RTE55266.1, ECO:0000313|Proteomes:UP000267585};
RN [1] {ECO:0000313|EMBL:RTE55266.1, ECO:0000313|Proteomes:UP000267585}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GUO666 {ECO:0000313|EMBL:RTE55266.1,
RC ECO:0000313|Proteomes:UP000267585};
RA Guo J., Sun J.;
RT "Arenibacter aquaticus sp.nov., a marine bacterium isolated from surface
RT seawater in the South China Sea.";
RL Submitted (NOV-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RTE55266.1}.
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DR EMBL; RQPJ01000001; RTE55266.1; -; Genomic_DNA.
DR OrthoDB; 9803237at2; -.
DR Proteomes; UP000267585; Unassembled WGS sequence.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd06127; DEDDh; 1.
DR CDD; cd04485; DnaE_OBF; 1.
DR Gene3D; 1.10.150.870; -; 1.
DR Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00594; polc; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32294:SF0; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF00929; RNase_T; 1.
DR SMART; SM00479; EXOIII; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
PE 4: Predicted;
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000313|EMBL:RTE55266.1};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:RTE55266.1}.
FT DOMAIN 1..192
FT /note="Exonuclease"
FT /evidence="ECO:0000259|SMART:SM00479"
FT DOMAIN 262..367
FT /note="Polymerase/histidinol phosphatase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00481"
SQ SEQUENCE 1478 AA; 167728 MW; CCAAE10674F63A42 CRC64;
MYLIFDTETT GLPKRWDAPY TDTDNWPRCI QIAWQLHDDM GRVIESQDYL VQPDGFNIPY
DAEQIHGIST ELAQQDGVPI GEVLEKFNAA LSKTKFVVGQ NVGFDLNIMG AEFHRYEVEN
SLQELPVLDT CTEHTAQLCQ IPGGRGGKFK LPTLTELHQY LFGEPFGEAH NATADVEATT
RCFLELVRRK EYTAEQLDVG PDYFKNFSEA NPQEIQLIGL KHINLKKASK KIADALWEKD
TGGISKEEIK ENLATLETAN FAHLHNHTQF SILQSTMSIP ALISATAKAK MPAVAMTDHA
NMMGAFHFVN GVINHNKGVV ARNEENLKRY EATKNGTLEE GQEPLEELPE PEVEITPIIG
CEFQVCEDHT NKSQKDNGYQ IVMLAKNKRG YLNLAKMSSI AFVDGKYYVP RIDKKVIEQY
KEDIIVLTGN LYGEVPSKVL NVGENQAEEA LIWWKETFGD DLYIEIMRHG QEDEDRVNQV
LLKFAEKHNV KLVATNNTYY QEKADAHAHD ILLCVKDGEK QATPIGRGRG YRYGLPNQEY
YFKSSEEMKE LFKDIPEAIT NIQEVIDKVE TFTLARDVLL PAFDIPEEFR FEEDKLDGGK
RGENKYLRHI TYEGAKKRYG EITPEIDERL DFELKVIEKT GYPGYFLIVE DFIRAAREMD
VSVGPGRGSA AGSAVAYCLW ITNLDPIKYD LLFERFLNPD RVSMPDIDID FDDEGRGRVM
DYVIDKYGAN QVAQIITYGT MAAKSSIRDT ARALDLPLGD ADRMAKLIPN MSKLKKIIGV
DEKKLKEKFN SEEIAKINEL LAISEGDDLE SETLNQAYVL EGSVRNTGIH ACGVIITPDD
ITKFVPVALA KDSDMYCTQF DNSVVESAGL LKMDFLGLKT LTLIKDTVKI VKAKHGVELD
PENFPLDDQK TYELFQRGET VGVFQYESPG MQKHMRSLKP TVFADLIAMN ALYRPGPMEY
IPSFIARKHG TEEIVYDLEA CEEYLAETYG ITVYQEQVML LSQKLADFTK GEADVLRKAM
GKKQKHVLDK MKPKFIEQAS KKGHAVDKLE KIWKDWEAFA AYAFNKSHST CYAWIAYQTA
YCKAHYPAEY MAAVLSNNMN DIKQVTFFME ECKRMGLDVL GPDVNESYYK FAVNDAGAVR
FGMGAVKGVG RGAVETIVEN RKEGGPFKSV FDLAKRIDLR AANKKAFENL AVAGGFDSFG
DTHRAQYFYE EGDGITFLEK AMKYGAKFQE NENSSQVSLF GEASEVQIPE PVVPPCEEWG
TMEKLRREKE VVGIYISGHP LDDFNAEIKA FCNASLSNLN DLEAIVNREV SFAGVITDVQ
HRVSKNGKGW AMFTMEDYTD SFEFRMFGEE YLKYRHFLMI NSFAYVKVFV REGWVNRDTG
KKGDPRMQFN MIMMLQDVME TFAKKLTIKL DIAQLREENI LELKDTLVSH QGNHPLSFVV
YEMEEQIKVN MTSRKQKIHI SSELLLELNK QDVLYKLN
//
