ID A0A3S0BMF7_9BACL Unreviewed; 387 AA.
AC A0A3S0BMF7;
DT 10-APR-2019, integrated into UniProtKB/TrEMBL.
DT 10-APR-2019, sequence version 1.
DT 27-MAR-2024, entry version 16.
DE SubName: Full=Muconate cycloisomerase {ECO:0000313|EMBL:RTE09870.1};
GN ORFNames=EJQ19_10065 {ECO:0000313|EMBL:RTE09870.1};
OS Paenibacillus whitsoniae.
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX NCBI_TaxID=2496558 {ECO:0000313|EMBL:RTE09870.1, ECO:0000313|Proteomes:UP000276128};
RN [1] {ECO:0000313|EMBL:RTE09870.1, ECO:0000313|Proteomes:UP000276128}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MER 54 {ECO:0000313|EMBL:RTE09870.1,
RC ECO:0000313|Proteomes:UP000276128};
RA Seuylemezian A., Vaishampayan P.;
RT "Bacillus ochoae sp. nov., Paenibacillus whitsoniae sp. nov., Paenibacillus
RT spiritus sp. nov. Isolated from the Mars Exploration Rover during
RT spacecraft assembly.";
RL Submitted (DEC-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|PIRSR:PIRSR633977-3};
CC Note=Binds 2 magnesium ions per subunit.
CC {ECO:0000256|PIRSR:PIRSR633977-3};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RTE09870.1}.
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DR EMBL; RXHU01000025; RTE09870.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3S0BMF7; -.
DR OrthoDB; 9775391at2; -.
DR Proteomes; UP000276128; Unassembled WGS sequence.
DR GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR Gene3D; 3.20.20.120; Enolase-like C-terminal domain; 1.
DR Gene3D; 3.30.390.10; Enolase-like, N-terminal domain; 1.
DR InterPro; IPR034593; DgoD-like.
DR InterPro; IPR036849; Enolase-like_C_sf.
DR InterPro; IPR029017; Enolase-like_N.
DR InterPro; IPR029065; Enolase_C-like.
DR InterPro; IPR033977; Galactarate_dehydratase_2.
DR InterPro; IPR013341; Mandelate_racemase_N_dom.
DR PANTHER; PTHR48080; D-GALACTONATE DEHYDRATASE-RELATED; 1.
DR Pfam; PF13378; MR_MLE_C; 1.
DR Pfam; PF02746; MR_MLE_N; 1.
DR SFLD; SFLDG00309; galactarate_dehydratase; 1.
DR SFLD; SFLDF00162; galactarate_dehydratase_2; 1.
DR SUPFAM; SSF51604; Enolase C-terminal domain-like; 1.
DR SUPFAM; SSF54826; Enolase N-terminal domain-like; 1.
PE 4: Predicted;
KW Isomerase {ECO:0000313|EMBL:RTE09870.1};
KW Magnesium {ECO:0000256|PIRSR:PIRSR633977-3};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR633977-3};
KW Reference proteome {ECO:0000313|Proteomes:UP000276128}.
FT DOMAIN 20..121
FT /note="Mandelate racemase/muconate lactonizing enzyme N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF02746"
FT DOMAIN 149..360
FT /note="Enolase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF13378"
FT ACT_SITE 90
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR633977-1"
FT ACT_SITE 164
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR633977-1"
FT BINDING 15
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR633977-2"
FT BINDING 42
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR633977-3"
FT BINDING 45
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR633977-3"
FT BINDING 89
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR633977-2"
FT BINDING 193
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR633977-3"
FT BINDING 219
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR633977-3"
FT BINDING 244
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR633977-3"
FT BINDING 294
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR633977-2"
FT BINDING 295
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR633977-3"
FT BINDING 383
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR633977-2"
FT SITE 162
FT /note="Increases basicity of active site Tyr"
FT /evidence="ECO:0000256|PIRSR:PIRSR633977-4"
SQ SEQUENCE 387 AA; 43271 MW; F98C0AC0C155F54A CRC64;
MKITNILLDV VSIPRHTGFV CQHAIVRVMT DEGIVGIGEM SDFSHLPKYS VDVRDLQATL
QASLVGRDPF ELIAINREMA EMFPETMYYY EKGTFIRCGI DIALHDLVGK ALGVNISTLL
GGRVRDKLKV CYPIFRSRTL EEVDANVDVV RKRLAQGFDV FRLYAGVNPD ADEAFLDRVR
QEFGSRVTIK SLDYSHLLDW KSALRTTKRL EPYGVQLVES PAYRNDFEGL RNFRMGVDLP
VSEHVWSFKQ LQDMIRYDSV DIFNIALIFI GGFQAARKAA AAAEISGKGV LIGTTQELSF
GTAAQAIFGA SLTNLNAISD PTGPELYVDD VTTEPVRYEN GYLLVPEADK PGLGMDIDWE
KVERLRVEGF SWGAQKVANL QDRTRGS
//
