ID A0A3S0R3A9_9SPHN Unreviewed; 1148 AA.
AC A0A3S0R3A9;
DT 10-APR-2019, integrated into UniProtKB/TrEMBL.
DT 10-APR-2019, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
DE AltName: Full=DNA 3'-5' helicase II {ECO:0000256|ARBA:ARBA00034923};
GN Name=addA {ECO:0000313|EMBL:RUN77233.1};
GN ORFNames=EJC47_07020 {ECO:0000313|EMBL:RUN77233.1};
OS Sphingomonas sp. TF3.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC Sphingomonadaceae; Sphingomonas.
OX NCBI_TaxID=2495580 {ECO:0000313|EMBL:RUN77233.1, ECO:0000313|Proteomes:UP000275325};
RN [1] {ECO:0000313|EMBL:RUN77233.1, ECO:0000313|Proteomes:UP000275325}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TF3 {ECO:0000313|EMBL:RUN77233.1,
RC ECO:0000313|Proteomes:UP000275325};
RA Afonin A., Vasilieva E., Akhtemova G., Zhukov V.;
RT "The Draft Genome Sequence of a Sphingomonas sp strain TF3.";
RL Submitted (DEC-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RUN77233.1}.
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DR EMBL; RWKS01000006; RUN77233.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3S0R3A9; -.
DR Proteomes; UP000275325; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 4.
DR InterPro; IPR014151; DNA_helicase_AddA.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR NCBIfam; TIGR02784; addA_alphas; 1.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000275325}.
FT DOMAIN 10..505
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 510..794
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 943..970
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 31..38
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1148 AA; 123303 MW; 1DCD950DB0901210 CRC64;
MTEGHSRRPL PVLKDAQARA SDPGGHVWLS ASAGTGKTQV LTARVYRLLL RGVDPGAILC
LTFTKAGAAE MAGRIGERLA AWVRMPEPDL VADLVALGED PSPQLRARAR TLFAKVLDAP
GGGIRIQTIH GFCQSLLAAF PVEAGLVPGF RPIEAREEAV MARDALAEML VTAEAEGRDA
TVTTVGALSL RLGEGEAERF LLACARAPAA MAELPMGAGI GPFIRRALDL PVGEIEAEIA
DACGDNAFDV DAVARLAAAN RAWGTATGGN HADIAECWIE AAPEMRARML GDLIGVPFTA
KREPRKYSPK LLAADPDYAD LAIEVGEACA HLLGLRERAA YADLLAAALA VGREYAAAYD
QAKRRLGAVD FDDLIRATIA LLGQPGMGEW VRYKLDQATE HVLIDEAQDT NPDQWAIIRA
LVEEFFAGEG SHDERVRTLF TVGDFKQAIF GFQGTDPKFF AAAEQRIGTL ARDAWAADEE
QRRQSGTMRA RPWHRLSLTQ SFRSTRPVLE FVDAAIEALP EPGLGAIADR EVHASEVPGP
GTVTLWPLVV AGGSEEDEES WISDSTRELA RRIARTIKGW IGTLMLESKG RTLRPEDIMI
LVKRRGELAS LIVARLYAEG VPVAGVDRLR LNAPLSVQDL LAAIRFVLQP DDDLSLASLL
VSPLIGWSQE RLMHAAPKRA VSLWRHIARA EPRAEIAPLF AMLDRADIAT PYQFLEELLS
GPLGGRRNLV RRLGQEARDP IEELLSAALD FEALGTPSLQ RFLDWFDRGE VEIKRDPSAP
LDAVRVMTAH GAKGLQAPLV ILADAAVDPT RSPRSTLKWW AGDMAAELPV IRPRKGEATG
PLAALVEETA ARDLEEHWRL FYVAATRAEE RLVIAGSVGP RAQGEAPAQS WYAAAARAMA
ALEVPEGEER SFTGRVPQKP VAPDRKAARA AVVAEPVPDW ARRAAPVEAR PPRPLAPSSL
GDDAVADAPP TPAMRAAAER GRLIHALFER LPRVAPAKRA EVADRWLASA GAVADATARA
EIAAPVLAVL GDPGLMDLFG PDALAEAPIA AVLPDGFVVS GTVDRLLVTP DLVSVVDFKT
GRGVPATPDA IPPYHLRQMA AYVAALAVIF PERRVEAALL YTSAPVLHML SAPLLEAHKP
GFAAAEQS
//
