ID   A0A3S2VHX6_9BURK        Unreviewed;       897 AA.
AC   A0A3S2VHX6;
DT   10-APR-2019, integrated into UniProtKB/TrEMBL.
DT   10-APR-2019, sequence version 1.
DT   27-MAR-2024, entry version 14.
DE   RecName: Full=pullulanase {ECO:0000256|ARBA:ARBA00024062};
DE            EC=3.2.1.41 {ECO:0000256|ARBA:ARBA00024062};
DE   AltName: Full=Alpha-dextrin endo-1,6-alpha-glucosidase {ECO:0000256|ARBA:ARBA00029618};
DE   AltName: Full=Pullulan 6-glucanohydrolase {ECO:0000256|ARBA:ARBA00031076};
GN   ORFNames=EOD73_00925 {ECO:0000313|EMBL:RVT87623.1};
OS   Inhella crocodyli.
OC   Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC   Sphaerotilaceae; Inhella.
OX   NCBI_TaxID=2499851 {ECO:0000313|EMBL:RVT87623.1, ECO:0000313|Proteomes:UP000288587};
RN   [1] {ECO:0000313|EMBL:RVT87623.1, ECO:0000313|Proteomes:UP000288587}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CCP-18 {ECO:0000313|EMBL:RVT87623.1,
RC   ECO:0000313|Proteomes:UP000288587};
RA   Chen W.-M.;
RL   Submitted (JAN-2019) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan,
CC         amylopectin and glycogen, and in the alpha- and beta-limit dextrins
CC         of amylopectin and glycogen.; EC=3.2.1.41;
CC         Evidence={ECO:0000256|ARBA:ARBA00023965};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:RVT87623.1}.
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DR   EMBL; SACM01000001; RVT87623.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A3S2VHX6; -.
DR   OrthoDB; 9800174at2; -.
DR   Proteomes; UP000288587; Unassembled WGS sequence.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd11341; AmyAc_Pullulanase_LD-like; 1.
DR   CDD; cd02860; E_set_Pullulanase; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   Gene3D; 2.60.40.1130; Rab geranylgeranyltransferase alpha-subunit, insert domain; 1.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR004193; Glyco_hydro_13_N.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   InterPro; IPR024561; Pullul_strch_C.
DR   InterPro; IPR040671; Pullulanase_N2.
DR   PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR   PANTHER; PTHR43002:SF11; PULLULANASE 1, CHLOROPLASTIC; 1.
DR   Pfam; PF02922; CBM_48; 1.
DR   Pfam; PF11852; Pullul_strch_C; 1.
DR   Pfam; PF17967; Pullulanase_N2; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF81296; E set domains; 2.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   4: Predicted;
KW   Reference proteome {ECO:0000313|Proteomes:UP000288587};
KW   Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           23..897
FT                   /note="pullulanase"
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5018684455"
FT   DOMAIN          389..754
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
SQ   SEQUENCE   897 AA;  97639 MW;  2143CB7142B8FF31 CRC64;
     MKAKARWAWA TWGALWVLPA WAQGDLAACD EARTPFAQSL PHAGAAVLPA QAHWFGAEQL
     RWPGQPADGR YRLLHSAQGG LQVAVGEPAS GFAQALPLSA SSAPVPAAFA WVGAGATLQF
     AQPLDRAARL ALLQGQSVLV REDEQGRVLA ATRLQAAGAL DALFADAAEP LAFGIARRPG
     ATEGRLWAPT ARAVEVCVYP GAQAAPSQRF AARWDAASGS WHWQQPVDLQ GQFVNYLVEV
     WVDGQGWVRQ RVTDPYAVSL NANSRRTAVL DLADPRTQPP GWRAPAGVRE IKRLNEMVVY
     ELHVRDFSRD DPSVRAAWRG KYPAFMEARS QGMRHLRDLA RVGVTDVHLL PVFDLATVPE
     KGCIEPQLPK APPNSPLPQA AVMAVAGQDC FNWGYDPFHF NAPEGSYATD ADDPAVRVRE
     FRQMVLALHR AGLRVGMDMV YNHTTAAGQS ERSVLDRVVP GYYQRLNANG EVEKSTCCDN
     TATEHRMMAK LMSDSLLLWS REYKLDSYRF DLMGHQPRAA MLAMRERLRR ELGREILFIG
     EGWNFGEVAN GARFVQASQL SLKGDGIGTF SDRGRDAARG GSFGSVKELM GSKGWLNGLL
     DDPAKRADAL KAADLLRLQL AGTLAEFRFT THTGATQRGA EIDYAGQPAG YAAQPGEAVH
     YVENHDNHTL FDANVLKLPA TASMAERLHR QTLGNALVAW SQGTVYVHAG QELLRSKSLD
     RNSYDSGDAF NRLDYGLRDN GFGHGLPPAP DNGRDWDTIA PLLGDPKLQP TPAQLRQAVA
     AFHDVMRIRA SSSLFSLPSA DEVQKRLRFH AAGPGQDPAL IVAELDGAGW PGARFGGLLL
     VFNGASQARE FSVPGAWRLH PVHTAPQAAD RRVRLEAQNA HAGALRVPAW GAVAFVR
//