ID   A0A3S3V7J6_9SPHI        Unreviewed;       613 AA.
AC   A0A3S3V7J6;
DT   10-APR-2019, integrated into UniProtKB/TrEMBL.
DT   10-APR-2019, sequence version 1.
DT   24-JAN-2024, entry version 18.
DE   SubName: Full=Beta-galactosidase {ECO:0000313|EMBL:RWY47249.1};
GN   ORFNames=EPL05_22450 {ECO:0000313|EMBL:RWY47249.1};
OS   Mucilaginibacter gilvus.
OC   Bacteria; Bacteroidota; Sphingobacteriia; Sphingobacteriales;
OC   Sphingobacteriaceae; Mucilaginibacter.
OX   NCBI_TaxID=2305909 {ECO:0000313|EMBL:RWY47249.1, ECO:0000313|Proteomes:UP000286701};
RN   [1] {ECO:0000313|EMBL:RWY47249.1, ECO:0000313|Proteomes:UP000286701}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=F01003 {ECO:0000313|EMBL:RWY47249.1,
RC   ECO:0000313|Proteomes:UP000286701};
RA   Yan Y.-Q., Du Z.-J.;
RT   "Mucilaginibacter antarcticum sp. nov., isolated from antarctic soil.";
RL   Submitted (JAN-2019) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC       {ECO:0000256|ARBA:ARBA00009809, ECO:0000256|RuleBase:RU003679}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:RWY47249.1}.
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DR   EMBL; SBIW01000028; RWY47249.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A3S3V7J6; -.
DR   OrthoDB; 703126at2; -.
DR   Proteomes; UP000286701; Unassembled WGS sequence.
DR   GO; GO:0004565; F:beta-galactosidase activity; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   Gene3D; 2.60.120.260; Galactose-binding domain-like; 2.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   InterPro; IPR026283; B-gal_1-like.
DR   InterPro; IPR048912; BetaGal1-like_ABD1.
DR   InterPro; IPR048913; BetaGal_gal-bd.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR   InterPro; IPR001944; Glycoside_Hdrlase_35.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR23421:SF165; BETA-GALACTOSIDASE; 1.
DR   PANTHER; PTHR23421; BETA-GALACTOSIDASE RELATED; 1.
DR   Pfam; PF21317; BetaGal_ABD_1; 1.
DR   Pfam; PF21467; BetaGal_gal-bd; 1.
DR   Pfam; PF01301; Glyco_hydro_35; 1.
DR   PIRSF; PIRSF006336; B-gal; 1.
DR   PRINTS; PR00742; GLHYDRLASE35.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF49785; Galactose-binding domain-like; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Reference proteome {ECO:0000313|Proteomes:UP000286701};
KW   Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           22..613
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5018618265"
FT   DOMAIN          35..349
FT                   /note="Glycoside hydrolase 35 catalytic"
FT                   /evidence="ECO:0000259|Pfam:PF01301"
FT   DOMAIN          394..504
FT                   /note="Beta-galactosidase 1-like first all-beta"
FT                   /evidence="ECO:0000259|Pfam:PF21317"
FT   DOMAIN          526..584
FT                   /note="Beta-galactosidase galactose-binding"
FT                   /evidence="ECO:0000259|Pfam:PF21467"
FT   ACT_SITE        182
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR006336-1"
FT   ACT_SITE        257
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR006336-1"
SQ   SEQUENCE   613 AA;  69185 MW;  C3F674E02D7E9182 CRC64;
     MLKKIIIYCA LFLSVIALSN AQTVTHTFAF GDNDFLLDGK PLQMISGEMH YTRIPREYWR
     ERMKMAKAMG LNTIGTYVFW NAHEMVQGKY DFTGNNDIAE FVRIAKEEGL WVVLRPSPYV
     CAEWEFGGYP WWLLKDKDMK VRSKDGPFIN AYRSYMLEVG KHLAPLQINH GGNILMVQIE
     NEYGSYRNDK EYLAQNEKIF REAGFDGLLF TCDGPSQMPA GYLPGFLPAV NGEDDPKKVK
     DLINKYHNGK GPYYIAEWYP GWFDSWGKPH SGSNAEADAK KLDEVLSAGI SINMYMFHGG
     STRDFMNGAN MSKSEPYSPQ TSSYDYDAPL DEAGNPTAKY NMLRAVIAKH LPTGQTLPDV
     PGKKRMTFAL EVINLTGKAN LFSNLGLPVE AEKPMSFEDL DQGYGFVLYR TTLKDAVSGL
     LKVKELRDYA TVYLNGKRIN VLDRRLKLDS LQLNSPTPNS VLDILVENNG RINYGPYLTD
     NRQGITDKVI LNGQELLGWK MYKFPFTTLA GMKYLPAQAG DEIAQPALYR GTFSLRGLGD
     TYLDMHGFGK GFVFINGHNL GKYWQIGPQQ TLYVPAAWLK KGTNEVVVFD QLKAGHTELS
     TFDHPVLNEL QKD
//