ID A0A3S4RB21_9ACTO Unreviewed; 1401 AA.
AC A0A3S4RB21;
DT 10-APR-2019, integrated into UniProtKB/TrEMBL.
DT 10-APR-2019, sequence version 1.
DT 27-MAR-2024, entry version 19.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=NCTC11636_01493 {ECO:0000313|EMBL:VEG28350.1};
OS Actinomyces howellii.
OC Bacteria; Actinomycetota; Actinomycetes; Actinomycetales; Actinomycetaceae;
OC Actinomyces.
OX NCBI_TaxID=52771 {ECO:0000313|EMBL:VEG28350.1, ECO:0000313|Proteomes:UP000266895};
RN [1] {ECO:0000313|EMBL:VEG28350.1, ECO:0000313|Proteomes:UP000266895}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCTC11636 {ECO:0000313|EMBL:VEG28350.1,
RC ECO:0000313|Proteomes:UP000266895};
RG Pathogen Informatics;
RL Submitted (DEC-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
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DR EMBL; LR134350; VEG28350.1; -; Genomic_DNA.
DR REBASE; 288602; Aho11636ORF1493P.
DR KEGG; ahw:NCTC11636_01493; -.
DR Proteomes; UP000266895; Chromosome 1.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 2.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR046816; MmeI_Mtase.
DR InterPro; IPR046820; MmeI_TRD.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR33841:SF1; ADENINE-SPECIFIC METHYLTRANSFERASE PGLX; 1.
DR PANTHER; PTHR33841; DNA METHYLTRANSFERASE YEEA-RELATED; 1.
DR Pfam; PF20473; MmeI_Mtase; 2.
DR Pfam; PF20466; MmeI_TRD; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Hydrolase {ECO:0000313|EMBL:VEG28350.1};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000266895};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 648..737
FT /note="MmeI-like DNA-methyltransferase"
FT /evidence="ECO:0000259|Pfam:PF20473"
FT DOMAIN 923..1022
FT /note="MmeI-like DNA-methyltransferase"
FT /evidence="ECO:0000259|Pfam:PF20473"
FT DOMAIN 1084..1263
FT /note="MmeI-like target recognition"
FT /evidence="ECO:0000259|Pfam:PF20466"
FT REGION 1..36
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1374..1401
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1401 AA; 152798 MW; 0B605B94E83754BD CRC64;
MGEGVMSQAR GTGRGSAGRS GRAADTGEAR AARTASQQHR EWLELVDTDG PFLSVPVMTG
LYPQGMPSLD RARREALRAA KSAFDRAWDA WDRDRELALA DYRRARDTWV STVLTEVIGW
GQGLVTAEAR PALAETYRVV SDGFPAVTVT PSGALARGET VGALLLLTDP VGSLREVGQD
GWAASPIDRM AAMLRSREST CTIGVVTDGR WWALVSAPPA GATASGIVDC QTWVEEPATR
NAFCQLLSVP RLVGGTSEKR LPALFADSVL AAEEVTDALG TQVRRAVELV VTALSEAART
APSAPALGDP LPEDAHEVYE AVVTIMMRAV FLLFAEERGL LPAQSLYTGG YGLAGVLDEL
EARARDEGEE SMDGTSLTWH RLLATSRALH DGVNAEDMRI PAYGGSLFDP ARFPFLTATD
DGGALRLTVS DRVMMHVLRS LQTARTGRGK EREVRRLSFR DIDVEQIGYI YEGLLGYTCA
RATETVLGLI GTDGEEPEVP LAVLEDLAEE HADDAELAAG VLTWVKEHQS AAKPPTKSAL
AKALRSGDAM DHDEAERALL AVTHEAGLRG RLRPWIGAIR RDLRGRPVVI EAGGLVVTET
PSRRDAGAHY TPRSLAEEVV RHALEPLVFH PGPHQTNDRS AWRPLSSDQI LGLHVADIAC
GSGAFLVAAA RFLARELVEA WTREGTLGRY VTQPGGAERH ALRQVVARCL YGVDINEMAV
EMCKLSLWLV SLDKDKPFSF VDDKIFVGNS LLGITDLRQL RTQHIDPSAA PTTRLFELDR
AGEHASALDV DTVLARVRER RSELASEVDN EDPARSTATK LRLQRENEED LRLLGSVADA
VVAVGLDPAV GAKPGKKLNE AYGDLAVALG RAFPPEGQAE DSMLEAILER GLTPTVSTDD
RRWRCLHWPL TVPEVMERGG FDAIVGNPPF LGGQKLTGAM GSNIRDWLVH ALANGQRGSA
DLVAYFFLRA AGLLCPSGTV GLIATNTLAQ GDTREVGLDQ MVARGFTITR AIQSRTWPAA
SANLEYAAVW GTRAEVGPAS EIVCDDEPVN RISTLLEPEG RVAGKPERIA ENAGIAFIGC
YVLGKGFILE PEEAQVWIER DPRNAEVLFP YLNGEDLNSR PDCSPSRWVI DFGMRSRDEA
GLYETPFARV VRDVKPQRDV VKIDYRREYW WRFAAWAPSM RRAIAELDEV LVVARVSKTV
MPVRVPTGAV MSDATVVFAT DSFSDQAVLS SSLHQAWAIK NGSGMRGDPR YTPTDVFETF
PRPAPTPALE AVGRSLDTER REIMLRRSLG LTTLYNLVND PGLTPGVDPD VDRLREIHRE
LDETVAAAYG WDDLDLAHGF HTYRQMTRWT VSPSARVEIL DRLLEENHRR AALEAQAAQA
PATRPRGGRR PQPVQEETLD V
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