ID A0A3S5EV86_9ACTN Unreviewed; 347 AA.
AC A0A3S5EV86;
DT 10-APR-2019, integrated into UniProtKB/TrEMBL.
DT 10-APR-2019, sequence version 1.
DT 24-JAN-2024, entry version 21.
DE RecName: Full=Glycerol-3-phosphate dehydrogenase {ECO:0000256|RuleBase:RU000439};
DE EC=1.1.1.94 {ECO:0000256|RuleBase:RU000439};
GN Name=gpsA_1 {ECO:0000313|EMBL:VEI03451.1};
GN ORFNames=C0Z10_00265 {ECO:0000313|EMBL:AZZ38444.1}, FEZ32_04940
GN {ECO:0000313|EMBL:QCV87804.1}, NCTC13652_01656
GN {ECO:0000313|EMBL:VEI03451.1};
OS Acidipropionibacterium jensenii.
OC Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
OC Propionibacteriaceae; Acidipropionibacterium.
OX NCBI_TaxID=1749 {ECO:0000313|EMBL:VEI03451.1, ECO:0000313|Proteomes:UP000277858};
RN [1] {ECO:0000313|Proteomes:UP000285875}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JS280 {ECO:0000313|Proteomes:UP000285875};
RA Deptula P., Laine P., Smolander O.-P., Paulin L., Auvinen P., Varmanen P.;
RT "Whole genome sequencing of Acidipropionibacterium jensenii strains JS279
RT and JS280.";
RL Submitted (DEC-2017) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:VEI03451.1, ECO:0000313|Proteomes:UP000277858}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCTC13652 {ECO:0000313|EMBL:VEI03451.1,
RC ECO:0000313|Proteomes:UP000277858};
RG Pathogen Informatics;
RL Submitted (DEC-2018) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:AZZ38444.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=JS280 {ECO:0000313|EMBL:AZZ38444.1};
RX PubMed=31671651;
RA Deptula P., Loivamaa I., Smolander O.P., Laine P., Roberts R.J.,
RA Piironen V., Paulin L., Savijoki K., Auvinen P., Varmanen P.;
RT "Red-Brown Pigmentation of Acidipropionibacterium jensenii Is Tied to
RT Haemolytic Activity and cyl-Like Gene Cluster.";
RL Microorganisms 7:0-E512(2019).
RN [4] {ECO:0000313|EMBL:QCV87804.1, ECO:0000313|Proteomes:UP000310120}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FAM 19038 {ECO:0000313|EMBL:QCV87804.1,
RC ECO:0000313|Proteomes:UP000310120};
RA Roder T., Wuthrich D., Sattari Z., von Ah U., Bar C., Ronchi F.,
RA Macpherson A.J., Ganal-Vonarburg S.C., Bruggmann R., Vergeres G.;
RT "The metagenome of a microbial culture collection derived from dairy
RT environment covers the genomic content of the human microbiome.";
RL Submitted (MAY-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=NADP(+) + sn-glycerol 3-phosphate = dihydroxyacetone phosphate
CC + H(+) + NADPH; Xref=Rhea:RHEA:11096, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57597, ChEBI:CHEBI:57642, ChEBI:CHEBI:57783,
CC ChEBI:CHEBI:58349; EC=1.1.1.94;
CC Evidence={ECO:0000256|RuleBase:RU000439};
CC -!- SIMILARITY: Belongs to the NAD-dependent glycerol-3-phosphate
CC dehydrogenase family. {ECO:0000256|ARBA:ARBA00011009,
CC ECO:0000256|RuleBase:RU000437}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP025570; AZZ38444.1; -; Genomic_DNA.
DR EMBL; CP040635; QCV87804.1; -; Genomic_DNA.
DR EMBL; LR134473; VEI03451.1; -; Genomic_DNA.
DR RefSeq; WP_028701948.1; NZ_LR134473.1.
DR AlphaFoldDB; A0A3S5EV86; -.
DR STRING; 1122997.GCA_000425285_00024; -.
DR GeneID; 82884424; -.
DR KEGG; aji:C0Z10_00265; -.
DR OrthoDB; 9812273at2; -.
DR Proteomes; UP000277858; Chromosome 1.
DR Proteomes; UP000285875; Chromosome.
DR Proteomes; UP000310120; Chromosome.
DR GO; GO:0009331; C:glycerol-3-phosphate dehydrogenase complex; IEA:InterPro.
DR GO; GO:0047952; F:glycerol-3-phosphate dehydrogenase [NAD(P)+] activity; IEA:UniProtKB-EC.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0046168; P:glycerol-3-phosphate catabolic process; IEA:InterPro.
DR GO; GO:0008654; P:phospholipid biosynthetic process; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR InterPro; IPR013328; 6PGD_dom2.
DR InterPro; IPR006168; G3P_DH_NAD-dep.
DR InterPro; IPR006109; G3P_DH_NAD-dep_C.
DR InterPro; IPR011128; G3P_DH_NAD-dep_N.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR PANTHER; PTHR11728; GLYCEROL-3-PHOSPHATE DEHYDROGENASE; 1.
DR PANTHER; PTHR11728:SF1; GLYCEROL-3-PHOSPHATE DEHYDROGENASE [NAD(+)] 2, CHLOROPLASTIC; 1.
DR Pfam; PF07479; NAD_Gly3P_dh_C; 1.
DR Pfam; PF01210; NAD_Gly3P_dh_N; 1.
DR PIRSF; PIRSF000114; Glycerol-3-P_dh; 1.
DR PRINTS; PR00077; GPDHDRGNASE.
DR SUPFAM; SSF48179; 6-phosphogluconate dehydrogenase C-terminal domain-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 3: Inferred from homology;
KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00022516};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098};
KW NAD {ECO:0000256|PIRSR:PIRSR000114-3, ECO:0000256|RuleBase:RU000437};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000256|RuleBase:RU000437};
KW Phospholipid biosynthesis {ECO:0000256|ARBA:ARBA00023209};
KW Phospholipid metabolism {ECO:0000256|ARBA:ARBA00023264};
KW Reference proteome {ECO:0000313|Proteomes:UP000277858}.
FT DOMAIN 5..167
FT /note="Glycerol-3-phosphate dehydrogenase NAD-dependent N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF01210"
FT DOMAIN 188..332
FT /note="Glycerol-3-phosphate dehydrogenase NAD-dependent C-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF07479"
FT ACT_SITE 199
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000114-1"
FT BINDING 8..13
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000256|PIRSR:PIRSR000114-3"
FT BINDING 108
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000114-2"
FT BINDING 148
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000256|PIRSR:PIRSR000114-3"
FT BINDING 266..267
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000114-2"
FT BINDING 266
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000256|PIRSR:PIRSR000114-3"
SQ SEQUENCE 347 AA; 35861 MW; 90642954757D2680 CRC64;
MPTFTVLGAG AMGTALCKPF IDAGWEVRLW GTWLDDHLID AIEAGQPHPR IKVAAAPGIR
TFRSGQLAEA LEGADVVDIA VTSEGLPRVT EMALDHIAGL RALCLTAKGF WTDPEGRIRL
LPDAIRAIAD QKGVSLPPLV AVGGPVKANE CAAGEVTATT YASRDLALAE TLAREAATDT
YGIRPSDDET GVELCAALKN VYAISLGIAD GLGEATGVPR HNLKAAAFAQ AIREMSLLVA
AVGGHSETPY GLAGVGDLEV TGLSGRNKLY GVRLGKGETP DGALAEMNRL EQTVEGYPAA
PLAVAFAHQA GADEGTLPLL HAVARILQGG VDDVYAAVAA AVKPARP
//
