ID A0A3S8ZAY5_9ACTO Unreviewed; 383 AA.
AC A0A3S8ZAY5;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 27-MAR-2024, entry version 19.
DE SubName: Full=Hydroxymethylglutaryl-CoA synthase {ECO:0000313|EMBL:AZN30632.1};
DE EC=2.3.3.10 {ECO:0000313|EMBL:AZN30632.1};
GN ORFNames=EJO69_10220 {ECO:0000313|EMBL:AZN30632.1};
OS Flaviflexus salsibiostraticola.
OC Bacteria; Actinomycetota; Actinomycetes; Actinomycetales; Actinomycetaceae;
OC Flaviflexus.
OX NCBI_TaxID=1282737 {ECO:0000313|EMBL:AZN30632.1, ECO:0000313|Proteomes:UP000270021};
RN [1] {ECO:0000313|EMBL:AZN30632.1, ECO:0000313|Proteomes:UP000270021}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KCTC 33148 {ECO:0000313|EMBL:AZN30632.1,
RC ECO:0000313|Proteomes:UP000270021};
RA Bae J.-W.;
RT "Complete genome sequence of Flaviflexus salsibiostraticola KCTC 33148.";
RL Submitted (DEC-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. HMG-CoA synthase
CC family. {ECO:0000256|ARBA:ARBA00007061}.
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DR EMBL; CP034438; AZN30632.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3S8ZAY5; -.
DR KEGG; fsl:EJO69_10220; -.
DR OrthoDB; 9769523at2; -.
DR Proteomes; UP000270021; Chromosome.
DR GO; GO:0004421; F:hydroxymethylglutaryl-CoA synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0006084; P:acetyl-CoA metabolic process; IEA:InterPro.
DR GO; GO:0010142; P:farnesyl diphosphate biosynthetic process, mevalonate pathway; IEA:InterPro.
DR CDD; cd00827; init_cond_enzymes; 1.
DR Gene3D; 3.40.47.10; -; 1.
DR InterPro; IPR013746; HMG_CoA_synt_C_dom.
DR InterPro; IPR013528; HMG_CoA_synth_N.
DR InterPro; IPR011554; HMG_CoA_synthase_prok.
DR InterPro; IPR016039; Thiolase-like.
DR NCBIfam; TIGR01835; HMG-CoA-S_prok; 1.
DR PANTHER; PTHR43323; 3-HYDROXY-3-METHYLGLUTARYL COENZYME A SYNTHASE; 1.
DR PANTHER; PTHR43323:SF2; HYDROXYMETHYLGLUTARYL-COA SYNTHASE; 1.
DR Pfam; PF08540; HMG_CoA_synt_C; 2.
DR Pfam; PF01154; HMG_CoA_synt_N; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000313|EMBL:AZN30632.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000270021};
KW Transferase {ECO:0000313|EMBL:AZN30632.1}.
FT DOMAIN 3..165
FT /note="Hydroxymethylglutaryl-coenzyme A synthase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF01154"
FT DOMAIN 175..248
FT /note="Hydroxymethylglutaryl-coenzyme A synthase C-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF08540"
FT DOMAIN 260..345
FT /note="Hydroxymethylglutaryl-coenzyme A synthase C-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF08540"
FT ACT_SITE 79
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-1"
FT ACT_SITE 111
FT /note="Acyl-thioester intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-1"
FT ACT_SITE 233
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-1"
FT BINDING 29
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-2"
FT BINDING 143
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-2"
FT BINDING 148
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-2"
FT BINDING 242
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-2"
FT BINDING 272
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-2"
SQ SEQUENCE 383 AA; 41332 MW; 0A57E3168B81C542 CRC64;
MSIGIHDLDV ATAHYVLDLD EMARALGVDP AKYEKGLGQD EFSVPAPDED IVTMAAEAAS
RVLSRNDPSK IRSVLFATET GIDQSKAAGV FVHGLLGLPN TVRTVEFKQA CYSGTAALQA
AVNQVARYPE DQVLVIASDV ARYAVDSGGE PTQGAGAVAM LITANPNLVV IEPGSGVYTS
DVNDFWRPND SSTPFVEGKL SLDAYMDANV GAWDDLAAQR GLAVTDIDRF MHHQPFTKMA
RKAHARLAEH TGVALGDDLI EDSMAYNRRI GNSYTASLYF GLAAQLHAEG DLAGKRLGLF
SYGSGSVAEF FTAVVQDGYS DHIHPENVAA SLDRRVPLTF EQYRELHAGY IGESDNVETP
VVTKAPFRFT GIVDRVRQYE ARG
//
