ID A0A3S9MZY5_9FLAO Unreviewed; 1066 AA.
AC A0A3S9MZY5;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 27-MAR-2024, entry version 17.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=EJ995_11630 {ECO:0000313|EMBL:AZQ44846.1};
OS Nonlabens ponticola.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Nonlabens.
OX NCBI_TaxID=2496866 {ECO:0000313|EMBL:AZQ44846.1, ECO:0000313|Proteomes:UP000279600};
RN [1] {ECO:0000313|EMBL:AZQ44846.1, ECO:0000313|Proteomes:UP000279600}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MJ115 {ECO:0000313|EMBL:AZQ44846.1,
RC ECO:0000313|Proteomes:UP000279600};
RA Choi H.S., Jung J.;
RT "Complete genome of Nonlabens sp. MJ115.";
RL Submitted (DEC-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP034549; AZQ44846.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3S9MZY5; -.
DR REBASE; 290585; Nsp115ORF11630P.
DR KEGG; noj:EJ995_11630; -.
DR OrthoDB; 32195at2; -.
DR Proteomes; UP000279600; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR Gene3D; 3.90.220.10; Adenine-n6-DNA-methyltransferase Taqi, Chain A, domain 2; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR046817; MmeI_N.
DR InterPro; IPR023135; N6_DNA_MeTrfase_TaqI_C.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR025931; TaqI_C.
DR PANTHER; PTHR33841:SF1; ADENINE-SPECIFIC METHYLTRANSFERASE PGLX; 1.
DR PANTHER; PTHR33841; DNA METHYLTRANSFERASE YEEA-RELATED; 1.
DR Pfam; PF20464; MmeI_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR Pfam; PF12950; TaqI_C; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Endonuclease {ECO:0000313|EMBL:AZQ44846.1};
KW Hydrolase {ECO:0000313|EMBL:AZQ44846.1};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Nuclease {ECO:0000313|EMBL:AZQ44846.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000279600};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 36..165
FT /note="MmeI-like N-terminal"
FT /evidence="ECO:0000259|Pfam:PF20464"
FT DOMAIN 468..689
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
FT DOMAIN 788..926
FT /note="TaqI-like C-terminal specificity"
FT /evidence="ECO:0000259|Pfam:PF12950"
FT COILED 1024..1051
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 1066 AA; 124065 MW; 1F59373A56896A9B CRC64;
MALFQTSVLK KYLAQQDTNA VDKAYRKYTK FFLNLEIQQN IDKSNEEQIQ ATFLTELFVN
VLDYTINPKP KYNLTTEYKN EKNNRKADGA IITEEKTVAV VELKGTNTKD LESIRRQAFD
YKVNHKGCRF VITSNFEKLR FYIDDTTELL DFDLFNLTRD DFGLLYLCLY KDNLLKELPL
QIKQDSLIKE EEITKEFYKD YSLFKRELYR DLVKKNGKVL KVALQEETGI TDNAEHQSDL
ERLEKNVKLT LFKKSQKLID RFLFIFFAED RGLLQPNYTI KRLAEWDTIR KLDVDEPLYD
RFKKHFNYLD KGRKGDAENK EIFAYNGGLF KPDSILDRVI IDDDLLYNHT KKLATYDFES
QVDVNILGHI FENSLNEIES INAEIEGGNF DKQKSKRKKD GVFYTPKYIT KYIVENTVGK
LCDDRKTELG FKEEEYFKGR GTGRKKRNDK TIQKLVETLD TYRDWLLQIT ICDPACGSGA
FLNQALDFLI KEHQYIDELK TKILGGGLQF PDIENTILEN NIYGVDLNEE SVEIAKLSLW
LRTAQPRRKL NDLSSNIKCG NSLIDSKTIA GDKAFNWETA FAKVFANGGF DVVIGNPPYG
IFLSDGEEAY YKKNYPLTSY KINLYILFIE RMFQIFEKGL IQFIIPKSLL FNTYFKDIRE
YLLTKSKIHE VFMISEKIFP DAEVGGSLIL KYEIVDNPNI SNEMKLISAD KYFDYDGQTA
LVNDISQEFF LSVPHFEISP IAEGANLIVK KLHTLKTIRE FYTLKNGLNP GNIKQILITD
EKLSDNHKPI IWGKELNTNS IQWGGQFVNY DYNIADNLNL DDIQSKKGMK KQTRIDFALR
TPELFENEKI VVRKTGDRFI AAKDNNNYYF DTLVHGIYEN NEEYSLDFLL TILNSQVATI
FYRLLHDIKG KVFAKISLDK LGAFPLPGTD FTQRNDISQY GLKLQKLQPE LNSLKVKFVK
YLFVKFQLLK TSRKLENWQD LDFGEFIKEL NKAIKATNKL RDKEGQAPVA VLTKTDEFEW
LDLFEQNKKK AQDLQSQINT LEQQIDAMVY QLYGLSDEEI RIVENG
//
