ID A0A3S9QAK2_9GAMM Unreviewed; 695 AA.
AC A0A3S9QAK2;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=ATP-dependent DNA helicase RecG {ECO:0000256|ARBA:ARBA00017846, ECO:0000256|RuleBase:RU363016};
DE EC=3.6.4.12 {ECO:0000256|ARBA:ARBA00012551, ECO:0000256|RuleBase:RU363016};
GN Name=recG {ECO:0000256|RuleBase:RU363016,
GN ECO:0000313|EMBL:AZQ86010.1};
GN ORFNames=EKO29_19610 {ECO:0000313|EMBL:AZQ86010.1};
OS Colwellia sp. Arc7-635.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Alteromonadales;
OC Colwelliaceae; Colwellia.
OX NCBI_TaxID=2497879 {ECO:0000313|EMBL:AZQ86010.1, ECO:0000313|Proteomes:UP000286937};
RN [1] {ECO:0000313|EMBL:AZQ86010.1, ECO:0000313|Proteomes:UP000286937}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Arc7-635 {ECO:0000313|EMBL:AZQ86010.1,
RC ECO:0000313|Proteomes:UP000286937};
RA Lin J.;
RT "Complete Genome Sequences of Colwellia sp. Arc7-635, a Denitrifying
RT Bacterium Isolated from Arctic Seawater.";
RL Submitted (DEC-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Critical role in recombination and DNA repair. Helps process
CC Holliday junction intermediates to mature products by catalyzing branch
CC migration. Has a DNA unwinding activity characteristic of a DNA
CC helicase with a 3'- to 5'- polarity. Unwinds branched duplex DNA (Y-
CC DNA). {ECO:0000256|RuleBase:RU363016}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|RuleBase:RU363016};
CC -!- SIMILARITY: Belongs to the helicase family. RecG subfamily.
CC {ECO:0000256|ARBA:ARBA00007504, ECO:0000256|RuleBase:RU363016}.
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DR EMBL; CP034660; AZQ86010.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3S9QAK2; -.
DR KEGG; cov:EKO29_19610; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000286937; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR CDD; cd17992; DEXHc_RecG; 1.
DR CDD; cd04488; RecG_wedge_OBF; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR004609; ATP-dep_DNA_helicase_RecG.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR045562; RecG_dom3_C.
DR InterPro; IPR033454; RecG_wedge.
DR NCBIfam; TIGR00643; recG; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF19833; RecG_dom3_C; 1.
DR Pfam; PF17191; RecG_wedge; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363016};
KW DNA damage {ECO:0000256|RuleBase:RU363016};
KW DNA recombination {ECO:0000256|RuleBase:RU363016};
KW DNA repair {ECO:0000256|RuleBase:RU363016};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|RuleBase:RU363016};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU363016};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363016}.
FT DOMAIN 285..450
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 484..630
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 695 AA; 77359 MW; DDE2653CE60C9B99 CRC64;
MEALTNLARV PVTTLKGVGP GMASKLEKIG LVSVQDLLFH LPLRYEDRTR ITDVRALMPG
MYTNIIGEVT QNEIVQGKRR MMLVTINDGT GSINLRFFHF SASQKNSLAV GLNIRCYGEV
NRGMRGYEMV HPEYKALEQD QPLSPAEETL TPVYSTTDGL RQISLRNLTE QALNRLQRGQ
VEELLPADIS QFQYSLAEAL ALIHRPPPDT SVVLMEEGKH PAQLRLIKEE LLAHNLSMLK
LRASSDHHPA VSLAIDEALE KKFLASLPFS PTGAQARVTK EIRQDLLKHM PMMRLVQGDV
GSGKTLVAAL AALSALGQGF QVALMAPTEI LAEQHAINFK KWFEPLNISV GWLVGKTKAK
ARRQALEAIA NGDMQMVIGT HALFQEQVIF NKLALIVIDE QHKFGVHQRL SLREKGAWQG
NYPHQLIMTA TPIPRTLAMT AYADLDTSVI DELPPGRTPI TTIALPDSRR DDVVERIRQG
CTNDNRQAYW VCTLIEESEV LQCQAAEDTA VYLQAQLSEL KIGLVHGRMK ADEKQAVMER
FKLGELDLLV ATTVIEVGVD VPNSSLMVIE NPERLGLAQL HQLRGRVGRG SVASHCVLMY
KAPLTKTATK RLNVLRESND GFVIAQKDLE IRGPGELLGT KQTGLADLKI ADLQRDAHLI
PEVQQHARLI WQKYPEKANA LIDRWLANRE KYSNA
//
