ID A0A401IQE4_9LACO Unreviewed; 724 AA.
AC A0A401IQE4;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 13-SEP-2023, entry version 15.
DE RecName: Full=DNA topoisomerase {ECO:0000256|ARBA:ARBA00012891};
DE EC=5.6.2.1 {ECO:0000256|ARBA:ARBA00012891};
DE AltName: Full=Omega-protein {ECO:0000256|ARBA:ARBA00032877};
DE AltName: Full=Relaxing enzyme {ECO:0000256|ARBA:ARBA00032235};
DE AltName: Full=Swivelase {ECO:0000256|ARBA:ARBA00030003};
DE AltName: Full=Untwisting enzyme {ECO:0000256|ARBA:ARBA00031985};
GN Name=topB {ECO:0000313|EMBL:GBG93758.1};
GN ORFNames=LFYK43_02170 {ECO:0000313|EMBL:GBG93758.1};
OS Ligilactobacillus salitolerans.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Ligilactobacillus.
OX NCBI_TaxID=1808352 {ECO:0000313|EMBL:GBG93758.1, ECO:0000313|Proteomes:UP000286848};
RN [1] {ECO:0000313|Proteomes:UP000286848}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=YK43 {ECO:0000313|Proteomes:UP000286848};
RA Tohno M., Tanizawa Y.;
RT "Draft genome sequences of Lactobacillus sp. YK43.";
RL Submitted (APR-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-independent breakage of single-stranded DNA, followed by
CC passage and rejoining.; EC=5.6.2.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000213};
CC -!- SIMILARITY: Belongs to the type IA topoisomerase family.
CC {ECO:0000256|ARBA:ARBA00009446}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GBG93758.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BFFP01000002; GBG93758.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A401IQE4; -.
DR OrthoDB; 9803554at2; -.
DR Proteomes; UP000286848; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003917; F:DNA topoisomerase type I (single strand cut, ATP-independent) activity; IEA:UniProtKB-EC.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR CDD; cd00186; TOP1Ac; 1.
DR CDD; cd03362; TOPRIM_TopoIA_TopoIII; 1.
DR Gene3D; 3.40.50.140; -; 1.
DR Gene3D; 1.10.460.10; Topoisomerase I, domain 2; 1.
DR Gene3D; 2.70.20.10; Topoisomerase I, domain 3; 1.
DR Gene3D; 1.10.290.10; Topoisomerase I, domain 4; 1.
DR InterPro; IPR000380; Topo_IA.
DR InterPro; IPR003601; Topo_IA_2.
DR InterPro; IPR023406; Topo_IA_AS.
DR InterPro; IPR013497; Topo_IA_cen.
DR InterPro; IPR013824; Topo_IA_cen_sub1.
DR InterPro; IPR013825; Topo_IA_cen_sub2.
DR InterPro; IPR013826; Topo_IA_cen_sub3.
DR InterPro; IPR023405; Topo_IA_core_domain.
DR InterPro; IPR003602; Topo_IA_DNA-bd_dom.
DR InterPro; IPR005738; TopoIII.
DR InterPro; IPR025589; Toprim_C_rpt.
DR InterPro; IPR006171; TOPRIM_domain.
DR InterPro; IPR034144; TOPRIM_TopoIII.
DR NCBIfam; TIGR01056; topB; 1.
DR PANTHER; PTHR11390:SF21; DNA TOPOISOMERASE 3-ALPHA; 1.
DR PANTHER; PTHR11390; PROKARYOTIC DNA TOPOISOMERASE; 1.
DR Pfam; PF01131; Topoisom_bac; 1.
DR Pfam; PF01751; Toprim; 1.
DR Pfam; PF13342; Toprim_Crpt; 1.
DR PRINTS; PR00417; PRTPISMRASEI.
DR SMART; SM00437; TOP1Ac; 1.
DR SMART; SM00436; TOP1Bc; 1.
DR SMART; SM00493; TOPRIM; 1.
DR SUPFAM; SSF56712; Prokaryotic type I DNA topoisomerase; 1.
DR PROSITE; PS00396; TOPOISOMERASE_I_PROK; 1.
DR PROSITE; PS50880; TOPRIM; 1.
PE 3: Inferred from homology;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000313|EMBL:GBG93758.1};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT DOMAIN 2..143
FT /note="Toprim"
FT /evidence="ECO:0000259|PROSITE:PS50880"
SQ SEQUENCE 724 AA; 81641 MW; 4C31512818CD10BB CRC64;
MTTVILAEKP SQARAYVEAL HHSTKKAGYY EVSDSILPAA TTITFGFGHL VELATPDKYD
RKYQRWSLKN LPIFPAHYKF VVPADKRKQF NVVRELLKQA DTIIIATDSD REGENIAWSI
MKQAKIDLKS KTLKRLWINS LEPEAIRDGL MNLRPGWDYY PAYQEAQTRQ ISDWLIGMNG
SPLYSLLLRK VGAAGVYSIG RVQTPTLYMV YQRDLSIKNF QSEPYLELQA KVAAQTAHFT
AQLEPNRRFK NENELQDWTR SKNVVAGPQP GVIRSVQKQN KKIASPRLFS LSSLQSEVNR
RFHVSASQTL KAVQALYEDK LLTYPRTDCN YITEKEFAYL VQNLSKYQTI AASQAVLNRT
QAQARYVNGA RVQEHHAIIM TKKVPTKAKL EALPSLQKKI YDLVLRTTLA MFAQPYEYQE
TTIITRVGQA DFKAVGKIPL KPGWKLLFAK DEQNSKNQTQ TLPEVEQGQK VSADVKSVQK
MTQPPKAFTE GTLITAMKTA GKTLDDEQAQ AILKDTEGIG TEATRANILE VLKKRKYLIT
QKNQLHVTAQ GMTLCQAVES EPLLTSPEMT AKWETALKQI GQNQRTQANF LHQIQQFIGK
MLEEVPQQLQ ANAELKQAVI SRKEMVQPAA GNSKVVMGTC PVCHEGQIVD KGKFYGCSNY
RAAAPCKFTL PKKWSSKTIP QTAVKALLIT GKTRLLKGFT SKKTGKKFAA KLVLKDQKLQ
FDFT
//
