ID A0A401KEC0_ASPAW Unreviewed; 620 AA.
AC A0A401KEC0;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 24-JAN-2024, entry version 19.
DE RecName: Full=General negative regulator of transcription subunit {ECO:0000256|PIRNR:PIRNR005290};
GN ORFNames=AAWM_00431 {ECO:0000313|EMBL:GCB17546.1};
OS Aspergillus awamori (Black koji mold).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus.
OX NCBI_TaxID=105351 {ECO:0000313|EMBL:GCB17546.1, ECO:0000313|Proteomes:UP000286921};
RN [1] {ECO:0000313|EMBL:GCB17546.1, ECO:0000313|Proteomes:UP000286921}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IFM 58123 {ECO:0000313|EMBL:GCB17546.1,
RC ECO:0000313|Proteomes:UP000286921};
RA Kusuya Y., Shimizu M., Takahashi H., Yaguchi T.;
RT "Aspergillus awamori IFM 58123T.";
RL Submitted (SEP-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Acts as component of the CCR4-NOT core complex, which in the
CC nucleus seems to be a general transcription factor, and in the
CC cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT
CC protein subcomplex negatively regulates the basal and activated
CC transcription of many genes. Preferentially affects TC-type TATA
CC element-dependent transcription. Could directly or indirectly inhibit
CC component(s) of the general transcription machinery.
CC {ECO:0000256|PIRNR:PIRNR005290}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|PIRNR:PIRNR005290}.
CC Nucleus {ECO:0000256|ARBA:ARBA00004123, ECO:0000256|PIRNR:PIRNR005290}.
CC -!- SIMILARITY: Belongs to the CNOT2/3/5 family.
CC {ECO:0000256|ARBA:ARBA00007682, ECO:0000256|PIRNR:PIRNR005290}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GCB17546.1}.
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DR EMBL; BDHI01000001; GCB17546.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A401KEC0; -.
DR STRING; 105351.A0A401KEC0; -.
DR Proteomes; UP000286921; Unassembled WGS sequence.
DR GO; GO:0030015; C:CCR4-NOT core complex; IEA:UniProtKB-UniRule.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000932; C:P-body; IEA:UniProtKB-UniRule.
DR GO; GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; IEA:UniProtKB-UniRule.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:InterPro.
DR Gene3D; 2.30.30.1020; CCR4-NOT complex subunit 2/3/5, C-terminal domain; 1.
DR InterPro; IPR038635; CCR4-NOT_su2/3/5_C_sf.
DR InterPro; IPR012270; CCR4-NOT_su3/5.
DR InterPro; IPR040168; Not2/3/5.
DR InterPro; IPR007282; NOT2/3/5_C.
DR InterPro; IPR007207; Not_N.
DR PANTHER; PTHR23326; CCR4 NOT-RELATED; 1.
DR PANTHER; PTHR23326:SF1; CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 3; 1.
DR Pfam; PF04153; NOT2_3_5_C; 1.
DR Pfam; PF04065; Not3; 1.
DR PIRSF; PIRSF005290; NOT_su_3_5; 2.
PE 3: Inferred from homology;
KW Activator {ECO:0000256|PIRNR:PIRNR005290};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|PIRNR:PIRNR005290};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|PIRNR:PIRNR005290};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000286921};
KW Repressor {ECO:0000256|ARBA:ARBA00022491, ECO:0000256|PIRNR:PIRNR005290};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|PIRNR:PIRNR005290};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015,
KW ECO:0000256|PIRNR:PIRNR005290}.
FT DOMAIN 3..232
FT /note="CCR4-Not complex component Not N-terminal"
FT /evidence="ECO:0000259|Pfam:PF04065"
FT DOMAIN 487..616
FT /note="NOT2/NOT3/NOT5 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF04153"
FT REGION 241..286
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 296..315
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 326..449
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 124..151
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 257..271
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 359..378
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 392..413
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 620 AA; 69643 MW; 9817C1CECC4AC70E CRC64;
MTSRKTQQEI DKTFKKVAEG IQTFEGIYEK IRSTSNITQR DKLEENLKRE IKKLQRYRDQ
IKSWASGNEV KDKGPLLEQR RAIETCMEQF KAVEKEMKTK AYSKEGLSAA SRLDPKEKEK
VETCDFLSNM VDELQQKIEA FEAEEETLHM QMKKGKKDVA KTNRLSDLSR LTERHKWHVN
KLELLLRSLQ NGNLEVAQVL DIKESIKYYV EDGHNIDYSG EDETLYDDLN LDNEAEVQFG
MTGDNDKVSS QDTQSIQDEE PEIKPKVNKG ESAGLRRPSA QMKSPLPVLA TLHPSTSTTA
ASGMKPAPPP TRLPGETLKY ASAAAAAAAS DKNGVGIAPL PPPPGASPAF PHAVPANKAS
ATASPSVASV QPTTSKAAPV LTIAAEDITG ARSKTPSVSP NVAAASTSSH TMDKVEAPAA
AKEQPATNGE ATSKQEEQEQ EQESEESIFH LPPGLQDLIQ SFEVTRSRAS ASAANASPSV
QRMLATSLAN CPEPADAEKP RHYKPQNPYN TPLYYPQEPL SIFDDPRLYE TGRIDTDTLF
YLFYYRQGSY QQYLAAKALK NQSWRFHKQY QTWFQRHEEP KTITEEFEQG TYRFFDYEST
WMNRRKADFK FVYKYLEDEL
//
