ID A0A401KPK7_ASPAW Unreviewed; 5098 AA.
AC A0A401KPK7;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 24-JAN-2024, entry version 18.
DE SubName: Full=Nonribosomal peptide synthetase 12 {ECO:0000313|EMBL:GCB21218.1};
GN ORFNames=AAWM_04103 {ECO:0000313|EMBL:GCB21218.1};
OS Aspergillus awamori (Black koji mold).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus.
OX NCBI_TaxID=105351 {ECO:0000313|EMBL:GCB21218.1, ECO:0000313|Proteomes:UP000286921};
RN [1] {ECO:0000313|EMBL:GCB21218.1, ECO:0000313|Proteomes:UP000286921}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IFM 58123 {ECO:0000313|EMBL:GCB21218.1,
RC ECO:0000313|Proteomes:UP000286921};
RA Kusuya Y., Shimizu M., Takahashi H., Yaguchi T.;
RT "Aspergillus awamori IFM 58123T.";
RL Submitted (SEP-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GCB21218.1}.
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DR EMBL; BDHI01000007; GCB21218.1; -; Genomic_DNA.
DR STRING; 105351.A0A401KPK7; -.
DR Proteomes; UP000286921; Unassembled WGS sequence.
DR GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR CDD; cd05918; A_NRPS_SidN3_like; 4.
DR CDD; cd19542; CT_NRPS-like; 2.
DR CDD; cd19534; E_NRPS; 1.
DR CDD; cd19545; FUM14_C_NRPS-like; 1.
DR Gene3D; 3.30.300.30; -; 4.
DR Gene3D; 1.10.1200.10; ACP-like; 4.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 5.
DR Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 4.
DR Gene3D; 3.30.559.30; Nonribosomal peptide synthetase, condensation domain; 6.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR NCBIfam; TIGR01733; AA-adenyl-dom; 4.
DR PANTHER; PTHR45527:SF1; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR45527; NONRIBOSOMAL PEPTIDE SYNTHETASE; 1.
DR Pfam; PF00501; AMP-binding; 4.
DR Pfam; PF00668; Condensation; 5.
DR Pfam; PF00550; PP-binding; 4.
DR SMART; SM00823; PKS_PP; 3.
DR SMART; SM01294; PKS_PP_betabranch; 1.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 4.
DR SUPFAM; SSF47336; ACP-like; 4.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 11.
DR PROSITE; PS00455; AMP_BINDING; 3.
DR PROSITE; PS50075; CARRIER; 4.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 1.
PE 4: Predicted;
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000286921}.
FT DOMAIN 754..830
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 1854..1931
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 3030..3106
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 4576..4652
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT REGION 1931..1960
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1994..2023
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1931..1954
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1994..2018
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 5098 AA; 560916 MW; 0D5DE606513F4DE7 CRC64;
MSRFSCIFPT LTDGYVPNLD HTRAAGRRTY TIDRSGWTAP SSQTESHILA AWGLVLSSYV
GTDEVAFYIV PTTGPDTTAL AELKVEGDMS RRSLINAAQQ LLHPGPVGAG QVSGEAANTI
ITFEKDIESL FVTQAEESFL SLHVHRDGQG HVSLSLTYHL SLLTDAQAAN VGTAMAQALA
EVGADDHNRL IKNLNLMSPT HLENIWQFNA NVPGMWEECF HDVIERRAAN RPHSLAVDAW
DMKLTYADLV REARLLAAYL QHRGVRPGSV VPISFERSGA ALVAMLAVSK AGGAFVSVPP
TLPAGRLDAI LEVIEAPFVV TWSKYESFWA ERLPTLPIDN YPKPSADATV EVLGKPEDLF
YVIFTSGSTG RPKGCMLSHS NWLNGALRNA PSWKYGPESR VLQMLSHTFD MSLLEICTSL
GSGACVCVPR TEEIETSISD AINRWQVNHV IMTPSLARSL RPDDVPGLKT MCLGGEAFPR
EIVTMWSERI NLWQFYGPSE CSINSSSRPI TRPDADPLNI GPPNSAACWV VDTQDYNKLV
PVGAIGELLV SGPIVGMGYL KNPIKTAEAF LDEVGFVAKD DPQFGGFRFY RTGDLVRWNS
DGTITFCGRA DTQVKLNGQR LELAEVEYQL GLEAGVQYAI AMAPQSGRCK NNLIAVLTVK
CSGASNQGNA ADEIPLLDRH DPIVQQTVKK LRSQLQHALP RYMVPTIWAF VGRMPMSPSG
KIDRVQLRNW VQEMSQETFD AITGRSCEAE DHVLGLSQLE QEIQLAWAEA LGLSAAEVGL
QQPFVALGGD SIKALDAVAR CRARQIKISM VHILSCEGVR EAASLAEVQE TPAHQVAEIA
VDYSDLWTCL STDYDISKLG VTQVEEVEDV FPCTTMQEGM FLGQIRRPGA YHMRFFHRVQ
LKGGSLPTVE RIQQAWASLV ERHPSLRTVF VDDLSSEAIY HSVVLRSVPM ELTMREVPRD
LNAEEALAMF TEELVPFRPN APLHRMLLLT CRGRVPYLML EISHVIMDGY ALSVFRREFI
RACSSTAPLP RGPDYRMFAN YHRTRQTDES AKYWTNYLSD CVPCHIPTDP LSVPPEGPPD
WPRTLQRRDF GFDNSAAFLQ RCKERQVTLA CAIRAAWALV LRAYTRSQDV CFGYVSSGRN
VPVPEVETIF GLCLSMQVCR AKLSEASTIA SLARKIQEDY VASLPFQHYP LAEAQRGLKQ
THGQGLFNTA ISMEWVPPTA EDEDALLDLE EIREQDDPTE YDIAISVDIH EGHIKLGFLY
WPNLSDFQIT HLAEALQGAM NCFAFQPDVA LDSLTLLQAS DLCSTLTNGP TLLPLEAVRG
NVISMIDRWA TRQPESPAID GWDGSLTYKQ LHEQSSWVAR NLLHQGVKLG DRILVCADRS
SRTVVTIIGL VRAGCVLVLS NPTDPEKRLQ WLAQKCNATL VIADPAYEER FATSGARVLS
TTSICAPAAW DYEFSALDEQ DLVSILFTSG STGTPKGILM DHGALATSVL LGHGRTLRFS
RHTRMLHFAS LTFDAALAEI FTTLAHGGCI CVPCEEDRLS DVSGCISRFA ANTAMLTPSV
GRLLDPEALP TLKALVMIGE PMSRLDVERF APVLDLYNGA GPTETSIMVT IAGPMKPSDE
PVNLGYAVAG VRLWVTEAEN PNRLAPLGAV GELIVEGRLV TRGYLDDPAR TKESFLPNLP
WLPSQHALYR TGDLVRYADD GSLRYMGRKD TQVKLRGQRI ELQEVEYHLR KSLPQAQVVV
EMVIPEGKIR AQASLVAFVS GLTAADVESS SARNFDQSMP MSQIALPGRT IQALEEALPR
YMIPSVYFAL DTIPLSVNGK ADRRRLREIG AVLLVSSTAH KNTVDGKSET VKWTATSELE
RTLLELWTTT LGLEAETIHG DDSFFELGGD SVSAMKLVAT ARDRFKLSLS VPQMFRHPTI
HQLAAILGEA TGQSESSASS TTEEGFTFST PDDSSNDGVD DDFLQLATAQ LAQLAQEKGK
KVDIAALLKQ LQGGSSSCKT PSVSSSSSLS SSSRKKKSAK VVSPVEAPAP VPVPFSLLDG
GADVVEKIRA QAVEQCKILP GDIEDIYPAT ALQEGMMALM ARTPGVYTTT LTCELPERVN
LARLHSAWDK AAEAHPILRT RIILTENNTA VQVVQRAKEL PWDTYSLQDG DPLPDLTSKM
TLGSTLLRLA EIHRQNQPQM LLVAIHHALY DGWSMPLLKQ AVEDVYHGQE LRPQPFTPFI
NYLNEGKPAA QVFWTAHLDG FAGSVFPNLP SINHHIQPTE RRTRSLVVPT ATPGSQYTMA
TKIQAAWAVT VSRYAEAEDI VFGTVSTGRS AAVPSIDRMV GPTITTVPVR ISLGNQAERL
TSLLQRVQED GWNRMDHEHL GLQHIRRLGE SAAAACSLQT LLVIQPREQP RAKSSSTLLS
GLQDVAELEG VDTYPLMLVC EPDGVSLHLT AVFDPAVLDA VMLDRMLAHW ELVLNQIWSE
PDMAVMGLDG VSYSDKQTLV RWNAGEKIAD GCAHDAVYEW SVRTPHAPAV FAWDGKWTYE
ELERCSSLIA RQVLAHGVSS GDFVALYHEK SRWAAAAILA VFKAGGILVT LDPAHPKDRI
KDILDQARPR LVMTSQSLFD KARELGTPVM DVQFAASQPM PEECFPLPTV SPTQVAYAPF
TSGSTGRPKG VPLEHRGLAA STASVARACL LRPASRVLHF ASFAFDASMM EHMIAWHAGS
CLCIPHETAR QTDLARCIQD FEVTWAFLTP SCLRLITLDD VQSLEALGLG GESMTSEDIS
IWGPRLRQIV QLYGPAECSI VAALTEVTKP SENRLIGRPN ACRCWVVDPH SPDRLAPLGA
VGELVIEGIT VGRGYIDDPE RTTQVFIPPP TWLQTLYPNE QQPSRLYRTG DLVRYAGTDG
KLTFIGRRDG QLKLHGQRIE LADVEAHLRP LIPGTQKVVV EMIHSVDNHH PLLAAFVEEI
LTSQDQVEQV VNLLHPSQTQ CALNVKVIDS ALSRTVPQYM IPRMYLHISR LPLGASGKLN
RRHLRELVAE FPRQTLNEYA AGSGLAVPNR PATAQEREMQ AIWARVLSVD PDTIGVNEDF
FRIGGDSISG MQVATRCNAA GMHITSADLF QHRTIEQLMR HLSANGKSGS ASISLPPEPV
DEWVPLAPIQ QLFFEIAPQG PDHFNQSLLL RTSRRVSAEN LAGGLDILVG RHSMLRARFC
RDDSGQWSQQ VRSRGPYPAS AFYRLTTHNH IAPELLPSLL TATQMALSIQ EGPLIAVDLV
NLMDDTQLVY LVAHHLIIDL VSWRILHGEL EEYLQTGSLA STTGSVPFLT WSRAQAEYSA
NHLTPTIALP GFQEANDSFD ASRYWGISRE SNTFGQTSTS TFTLDQTLTD QLFGPANNVL
DTRPAEVLQA ALWYSFTRSL TDRPGPSIYV EGHGREPWTG SIDLSGTVGW FTTMSPLVSA
PWDSLSQTSM RDFLDALSYI KDQRRRIPAN GWAYFTSRYL NDEGKVAYRM KPVVEILFNY
MGQYQEMNRE DAILQLAGDD IQSGTGAADV ADNVPRFSLI DVSAFISNGC LTFQFIFPKS
LQQDSRLEGC FQEYERTLVA AANSLSTEGP RKTLADFPLM PGLTYDQLSQ CLDHTLPSMG
LCARDVVDIY PCSPVQQGML LAQLRDRQAY QQRFKFQVKS RGSTDRLTLE KLKDAWTEVI
NRHDILRTLL LPVSDYSHLD QVVMAPGSLQ HLVRINAMDA NPTQGLPHSI NITCDSTGTV
ICEWNVGHAL VDAMSIAVIQ QEVNEALEGS LGQHQKAPRY ADYIQWLSLQ DNTETQAYWK
KYLEGVEPCL FPKLASSTDK VNPEGTISAI RATWTRDSRL DKLCHTHGIT LTNLFHIVWT
LLLSAYVGTD KVCFGYTTLG RDVPVDGVEK MVGPLVNVIA TTIQLQEDDS ILDALLTHQT
HLSNSLQHQH YALADVYASL GLVGSQLFNT IVSLQDISHF NAPDERPTRV EMLPANDVSE
YDVALNIGVD QSSIQVVCSY RTLSLSAEQA DALLRTTSHV LDEILRDPTQ PLRDLEVISP
ECKERLVKWN AAMPAPTVEY IHEKIQDQCR LHSSREAVYA WDGIFTFAEV DDLSSRLAAR
LIRMGVTSGH IIPIYSPKSR WTVIAILGVL KTGATFTLLE TSHPMARLRV ICNEIKADII
IAPASHAVPA ATLAPILVVL DSITSMSPQE SDLLPAVGMP PAAEALAYLI FTSGSTGNPK
GVMVTHQNLC SNTSIITTSV NMTSDSRVLQ FASHAFDACL WEIFGALFAG ACLIIPSESE
NKEDLTDCIE RMVVTWAFLT PSVARILKPE SLTSLRNLVL GGEPIAASDL DMWRGHVQVV
CAYGPTETTI LASTTSPSTF PRDGKDIGVP TGSSLWIVDK QNYNKLAPLG ATGELLIEGP
NVSQGYLGDP EKTNEAFPVA PHWLSQLRKS PTRVYRTGDL VRFNTSTGTI HFVGRKDNQI
KFHGQRIELG DIEHHAQQAF SNASMVIVDL ITPEQPQQPY IVAFVHQPDT RTGTADPIET
ILLPPSESFR ADAIGAQNHM HKRLPHYMVP TAFLPLHRLP LSGTGKADRR RLRQCALSLS
SLELNAYRAT ASTKGMPFTT AERKMQELVA TVLGRDVSEI GMDDSFFYLG GDSIQAMRLV
SEGRQQGLTL SLQAIFDAPR LGDLAYRTTN LVRVSEPTPP TLLATSSDEC DHKETIVAAL
PIKKTDVAEV LPTTSFQRTW LDAQLKSYIV VDIPGPIDLA RLRKAILRVV KAHPILRASF
VPYETTTMQV ILRTAEVITE ADLSTTTVEG ICRQDANAPM APGTPYLRII LATQGEVDHK
LIMRLSHAQY DGISLSLLMN DLSHAYASES RPLPSSHFPS FNDYITYQQT QRADPTATTF
WHRLLKDVPI TYLDLQPAET STSNGSLITC TRDINIAAFP SLPNGITTAT AVKSAWSLVL
AQKTGSLAVI FGQVVHGRGI ALTEVEGIVG PCANITPVVA RLGLQTTGME LMQTLQDQHR
SAMPYEMVDL DDALAYTKDS QAGREKLQTI VQHQNNVMAD DMELSLGEAR CGVDVRAVDH
MPQEVWVYSS IDGGRPGMLE AKIMSSTLVL SEEVAEELMD LLVEMMKGLL SDPEGVCV
//
