ID A0A401L7L1_ASPAW Unreviewed; 5641 AA.
AC A0A401L7L1;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 24-JAN-2024, entry version 17.
DE SubName: Full=Nonribosomal peptide synthetase 8 {ECO:0000313|EMBL:GCB27465.1};
GN ORFNames=AAWM_10350 {ECO:0000313|EMBL:GCB27465.1};
OS Aspergillus awamori (Black koji mold).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus.
OX NCBI_TaxID=105351 {ECO:0000313|EMBL:GCB27465.1, ECO:0000313|Proteomes:UP000286921};
RN [1] {ECO:0000313|EMBL:GCB27465.1, ECO:0000313|Proteomes:UP000286921}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IFM 58123 {ECO:0000313|EMBL:GCB27465.1,
RC ECO:0000313|Proteomes:UP000286921};
RA Kusuya Y., Shimizu M., Takahashi H., Yaguchi T.;
RT "Aspergillus awamori IFM 58123T.";
RL Submitted (SEP-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GCB27465.1}.
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DR EMBL; BDHI01000029; GCB27465.1; -; Genomic_DNA.
DR STRING; 105351.A0A401L7L1; -.
DR Proteomes; UP000286921; Unassembled WGS sequence.
DR GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR CDD; cd05918; A_NRPS_SidN3_like; 5.
DR CDD; cd19542; CT_NRPS-like; 3.
DR CDD; cd19545; FUM14_C_NRPS-like; 2.
DR Gene3D; 3.30.300.30; -; 5.
DR Gene3D; 1.10.1200.10; ACP-like; 5.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 5.
DR Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 5.
DR Gene3D; 3.30.559.30; Nonribosomal peptide synthetase, condensation domain; 5.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR NCBIfam; TIGR01733; AA-adenyl-dom; 4.
DR PANTHER; PTHR45527:SF1; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR45527; NONRIBOSOMAL PEPTIDE SYNTHETASE; 1.
DR Pfam; PF00501; AMP-binding; 5.
DR Pfam; PF00668; Condensation; 5.
DR Pfam; PF00550; PP-binding; 5.
DR SMART; SM00823; PKS_PP; 4.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 5.
DR SUPFAM; SSF47336; ACP-like; 5.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 11.
DR PROSITE; PS00455; AMP_BINDING; 3.
DR PROSITE; PS50075; CARRIER; 5.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 3.
PE 4: Predicted;
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000286921}.
FT DOMAIN 773..849
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 1881..1957
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 2927..3003
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 4015..4091
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 5120..5196
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT REGION 5417..5438
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 5641 AA; 623747 MW; 29A82FCEA8C080D0 CRC64;
MGSMEDLPVG VFPTLVPPSS SGHTEFHARE ISPDILTNLL QSSIRKEVDL IVLLRSIWAI
VLRQYVESDG LCFKAGSMTS ACSELDMLVY QAGISSHDTP EGIWRQQQIT TGISAGGRNW
EVNTGVFLLK PCSDRQWRAR AMQAVDACLV MEEVDANAPR LYFLAKATLL SRQQVQYVAD
TVAHVASEIL RKPNQTLRDT GFFSPQHAPI LHKWNHRTFW PGEGDIPSSI LDAIRRHSQR
SPDALAIVAW DGSISYGDLE ARSTCLAEYL RRVGVGPGVM VPLLLDHSQW VIITQLAVLK
AGAAFVPLEP SHPQERLRYI VRCVKAFLIV ASEAHCARAR QLVEQVIPIN ESIMNTVRSA
ILSTACITPT ADAPAYVLFT SGSTGQPKGC VIDHRAMAQL PHDGVAASVG PDSRVLQFAT
YSFTMAIYEI YWCLSNGGTL CIPSDHDRLN NLPTVIEEMQ VTWAVLTPSV LRRLSHDNPP
SSLQTLALGG EPLAAGDMEA WVRKLQVFFG YASSEGTALN CLTELSEQSH ALEYTPTPRV
RHWIVDPDNH DKLAAMGTVG ELIVESPGLA REYLDNPTAT AAAFIQSPDW RRSFRFSQDG
SQPIRMYKTG DLFRYTERGN LQYVGRKATQ VKIRGQRLDL GEVEYNVSQQ CPAPARVIAE
TGVPRDGAGT MLLVAFLYSD NYTLEQSETT QSDEASLFAV PSDQFRADMK HLTQALEPLL
PAHMLPSNYI PLRRFPSTLT GKADRRLLRE LIQQHTRGEL ERYQSVTTNT VAPPSTRLER
DLHGLFAEVL GINAQDFGIH HSFISLGGDS LAAMKLVNLS RKLGLRLTVA QIMESKTVAK
LSNITLVDES SVSTIIPLRD GPLEAKLPLD FWEGIEEEHI EFISNCSTIQ QGILLGHIRN
PAHYHNRIVW EVLDPPAARD IDRMRRAWAH VCRRHPMLRT FFVEAPAGNS SMALQITLKV
SHFGPEDVDV LYEEPEQMPS TNPALASDQP LQWIPRLRIY EVTGNRLYCA IDIHHVQVDM
ISLGIIIRDL RRCYLDETEY VAAGDVSEPN SVSYTDYAQY LVASQSAESL SYWRIRLAHT
EPCIFPRLQQ GPFEAASSAL CSVELPLMPM ADHLQSFCKD TEVTVATVFK LAWSLVLSAY
TGSRCACYGS VTAMRDVPLA GVEDLVGPLI NTLPFCVELN PDERVMNVLR RVQNQFLEEL
PHRQVSIAEL QHSLKNSSGT LFNTTMSFVD SLDPAANSQH PIRLVSWYGP TEYDLVVEVT
PGPPVSCCIR YWSHMMSDWQ ASALADTLAM LVSQILRGAS SGQSVGSLDL CTARDKQKLV
EWNGSPPEAV RVRVHDLIKA RCIEQPDALA VWAWDGALSY RELDCLSSNL ARRLMARGVG
PEAFVGICFD RCKWTLVSVL AVAKAGGAFC LMDSSHPTYR LREICDALGA NLVITTPTHV
AKVSKCERPL LVVVEGVCSE TDDLPGGLGS EHRDTLPRNA LYAVFTSGST GKPKGVVITH
ENLAATIKNC PQRLDLRPSD RSLHFASYAF DFSIIEVIVP MVVGACVCIP SEHDRLNNLP
KAMADLAVTW AIMTPTVSRL LEPASLPTLR TLVLGGEAIM PYDFTTWQGY TTLISAYSPA
ECTPIGLCTV VQADDPNCLG KPFTSMSPWI VDPENVQRLV PVGAVGELVI EGTVVGRGYL
HDAEHRDIAS SPFIPPTRWL SQYRTIDVTN PSRLYRTGDL VQFTEDGAVR FIGRKDLQVK
IRGQRLELGE VESRLRSYLP WAGAIVVEAV VLKSPSGRAV LVAFISICSP DVTDNAETVC
EIAGSFDNDD NSVYQRDIDK ALSHLGDELP NWMVPSVVLK VQGIPMTKSG KVDRRRLRQL
ATLIPRETLG ASNAHSSLRQ APVTEEERRL QMAFANILQM RPEEIFADSH FFQLGGDSVG
AMELVAAARL QNMGISVSEI FQHPTLSELA AVATKTMDEE TSEIPPFSLL PSDYESDIIS
SALEQCALSL NDLGDIYPCT AFQEGLMALA SQKAGKYVCQ CLFELGRDVE INKLKTAWQA
TVTANDILRT RIIHVHPHGT FQVVLTPAAA PIFWHRYETV NEWRKKDMEL NMELGTTLAR
FSILSSAQGQ TLVGVSLHHA IYDGVSLPRL FDQVQRAYQN ERLPLNKFTP FIRSLILQNP
KDVEEFWRTE FHGLQTSVFP GLPEPGFVPN PTASVEKEIR FPRPPHLPSV TLSTVMRLAW
ALLVSYYTDS EDVVFGVTVS GRARSVPGIN LLTGPSMATV PFRTRICRDS LLLDALCDAQ
DHSMRMIPFE QAGLQRIREL SDEASVACGF QSQLVIQHGE HSQASPNMMI LRDQFADHAA
FLNYALVILC TPSTAHQTVR VNVQFDPQIV LPAVADRLLS QFAHIVDQMS YQPHTLIRFI
ISYCRRRAVE SWDGTLTYAE LNDLSGKLGQ HLLNRGLKQG SRVVLLFDRS LISVVTMLAV
LRSGGICVNI DPSLPQTRIT QTIHQARPSV ALVSSTYQSR LADALPVPVL TIALDTIPRS
AKNIPWPLVR STDLAFILFT SGTTGKPKGI MVEHGNCATD IFHYSAVLAV TPEIRTLHFC
SYAFDLSIYE VLTPWMHGGC LCIPSESARL SDLPGVIQKY HVNFAILTPS VGSALHPSDL
PSLQTLILGG EVMPAVMFDR WASHVRLINL YGPAEATQCA ALHLKPTDEF VPGLLGAMVG
ASGWVTVPGS PNCLTPIGAV GELLIEGPAV TRGYFDEMEQ TQASYIDPPA WLQEFRGPAE
PGRLFCSGDL VQYVPNRPGW LRYIGRKDKQ VKIRAQRVDL TEVEHHVDEL LPPGSQAIAE
VLFQHSSPRQ SSQLAVFLAF GEQLTIKMDI ICALDEPFRR LIAEIHPQLQ KSLPSYMVPT
LFFPVSHVPQ TPTGKTDRNR LRQLVEGWTT EQLLPYHPFS TDSARAPVET AEERIWQHMW
CEVLCIRPEN IARTSHFFHL GGESVRAMRL AGLAQREGWI ISVPDIFAHP TLSEMASFAV
PSPKGVPVPS PDRFSLVGSR RRASYLVNMA THQCNVTKSE IEDIYPCTPL QETLIASTVQ
KGGSYQALEA FDLIADVDLE RLEMAWQKTA ADHAILRTRI MQDYTGELFQ VVVKGPLAWT
VEQADKNPEN GLLHFQRTMG IGTPLIHLVL LQSPGSTIPD RLLLSIHHAL YDGWSLPLLL
EEVNQKYIGP CRGQDTTVPF NVFVSHVIKS MDRCCHFWKK ELETKTEARL FPRLPDGAYE
PHPQAILQDQ VSVSPTRNES GITLATMVQA SWALTVSCYL NASDVGFGIT TSGRAAPVPG
IDRLLGPTLV TLPRRIHIQN TLDIKLFLRQ LQQRFTEQSK YEHIGLQQIR QLGPSAASAC
RFQTLLVIQP DQPKRQLAWA GNHQSAAELT QFSNVALTLI CQLPSPSSTS IGLTAVYDPR
VLAPAQMQRV LSQVGHVFRE LQDAPDGVPI ASLNMLNPSD SVELQQWNKL TVPIGKHQDQ
ECVHDMIRRR CQLQPDAVAV EAWDGSLTYG ELDSYTEYLA AKLQLCGVRN DHFIALYFEK
SCWTIVAQLA VLKAGGALVM LEPSHPVDRR REICQTLDVM LILGPPHLTH SLEQELNIKV
LPISKAHLIV MDGEHLSVDS HIQSHNLMYA IATSGTTGCP KLALVHHGGF VATARTLVDR
LLVLASSRVL QFSSYGFDAS LVEQLLPLIV GGCVCVPTPF DRDNRLAFTM ATMKINWMVA
PASVIHLLSP DTVPTLQTLI SAGEPLQQSI IDVWATRVNL INAYGPAECS MVCSVNPSVR
PGTDPNNIGY PIGGTCWIVD PNDINTLLPI GAEGELLVEG PNVGVGYAKD PRRTRVAFSS
SPPHWLRSIH GDSVHTRIYR TGDIARYNPD GSVHYVGRKD SQIKLHGQRI ELAEVEQHVR
LCFPGAIQVV VEMVKFDDFE TSSLVALIVL SSSGFPASGS NILLPTAANF LNNVQHAEAL
LQDRIPLYMI PTLFVPVAQI PFNTNGKADR ARLRTLVASL SIHDRTGYRS TATTKPNTDL
EIVLHGIWSR VLRISPQKLG IHESFFRVGG NSISSMQLAA QCNEAGLPIS VQDIFRCRTI
SRLASSFKGH PVQADRSLEA HSQDGSLGPV QRLLPEVGHP KAPVDSTLAA FTDGQLEEIN
NGIPPGYEGQ IQDIYPCSHA QQGMLISNTR NSSLYQQVYR FEVVRGFDAP KVDCQQLVRA
WHAVVKRHGA LRTVFLDIPG RGFHQIVLVN ASDNVIIAAD GNHSSTCTQK RPGTQDLYGP
LPTLIIHQEY DERLWIELHI SHALLDGVSL RIIEQDLVKA YYNNLPSSLP DYRDYVTYLR
AKGDTEISLS YWKKYLEGAQ PCLFPRLRDS TAAVTDVSEP KATVDFVSLD LGPPKSLDQL
FWAIVLQQYT GTDSVCFGYA TSARHVPIPH VDEIVGPMIN TLPSQIQLSR DSSVLSTLQK
YTVHFMSCME HAYVSLGQIL HSAMSSTQSP FNTMITIVET GDRRDDADGS CLSLVGMDGH
GETEYALALT VSKASSRMDL HLSYQTSMVN ASQAHDVCLM FQKVLCEVLE RPTAKTGAID
ALNMGQRQRM ESRQPTPPAA ANTLIYETIN RRCAEQPSSS AVCAWDGEFT YGEIDRLSSV
LAGEILALGV KPEDPVLVCL EKSRWVPVVM LAVWKAGACL VLLDATHPTT RLHMIAKQSA
ASLAIANRST RSKANILCSR VLQIDDDRLW TRHVNSSRAD MVHVDGHSAA YIIFTSGSTG
TPKGAVIEHA SLSIAWETLI TRLQLNPTSR VLQFASHSWD ATIIDVTAAL MSGGCLCVPS
ENERMGHLAL AANRMRANWM LLTPTVARQL TPSEFLYLET LVLGGERVNT TDIEVWHDKV
CLLSAYGPAE CTQITSVTRP LDMTCDARNI GVPNASAGWV VRPDDPEQLV PDGAIGELLI
EGPIVGQGYL NEPQRTYQAF IEAPSWLQAI RSPGTVRVYR TGDLVRFAAD GSLIFIGRMD
DQVKLHGQRL ELGEVEHHVQ QAFAGSLTVI DLIQPGEEQQ MGPFLAACIF SPGHACKSPF
HAGIWEPPSD TFRKKVQEAR TALYQSLPSY MVPSVFLPLA CLPKATTGKI DRKRLRNYIL
NMSQEELLTY SSPDVNWAAS VLTLMETRLQ DHISSILHKQ PWQVSPDQDL FQLGMDSVLA
MALIARLYKD GLKLTVRDIF EQPQLRNLAL VVRETLRPDQ EGVRKAHSRS PLLHYAREIA
DEWKMALTSI EHILPTTHFQ RESLEARHKV YFIFHFHGKI DCQRLQHAVQ AAVDKYAILR
TVFIPWDEST VQITLGDATV PVEITTTDLN PVEIAGSICR EDSSAPVASG KLHTQLRLIM
GSNRDSMALR LSHAQYDGIS MPRLFTDIAR AYQGITLSPA KVDFPEYLHS RIYDIPSQAY
KFWRDLLRGS SMTYLRSESS SGSPPSHNRG SLITSTTEVS YPPAPAGTTM ATVAKAAWAL
CLAHMAGTHD IVFGQLVNGR NLELPEAETV VGACVNYVPV RVTLQADWTV SSLLHAVHQQ
QTRSMSFETL EYDELVAQCT SWPADTDPGT GIHYVNIPDA FAGSGLPGVG SDYETYVSRL
PYYHPRVTCS PLSKGTLELT LYVSDQLWDQ SQADQTLHLF GLTVVRILEN PTGLALDVLK
R
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