ID A0A401P7B4_SCYTO Unreviewed; 3891 AA.
AC A0A401P7B4;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE RecName: Full=DNA-dependent protein kinase catalytic subunit {ECO:0000256|ARBA:ARBA00018077};
GN ORFNames=scyTo_0008314 {ECO:0000313|EMBL:GCB68993.1};
OS Scyliorhinus torazame (Cloudy catshark).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Chondrichthyes;
OC Elasmobranchii; Galeomorphii; Galeoidea; Carcharhiniformes; Scyliorhinidae;
OC Scyliorhinus.
OX NCBI_TaxID=75743 {ECO:0000313|EMBL:GCB68993.1, ECO:0000313|Proteomes:UP000288216};
RN [1] {ECO:0000313|EMBL:GCB68993.1, ECO:0000313|Proteomes:UP000288216}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=30297745; DOI=.1038/s41559-018-0673-5;
RA Hara Y, Yamaguchi K, Onimaru K, Kadota M, Koyanagi M, Keeley SD, Tatsumi K,
RA Tanaka K, Motone F, Kageyama Y, Nozu R, Adachi N, Nishimura O, Nakagawa R,
RA Tanegashima C, Kiyatake I, Matsumoto R, Murakumo K, Nishida K, Terakita A,
RA Kuratani S, Sato K, Hyodo S Kuraku.S.;
RT "Shark genomes provide insights into elasmobranch evolution and the origin
RT of vertebrates.";
RL Nat. Ecol. Evol. 2:1761-1771(2018).
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC {ECO:0000256|ARBA:ARBA00004604}.
CC -!- SIMILARITY: Belongs to the PI3/PI4-kinase family.
CC {ECO:0000256|ARBA:ARBA00011031}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GCB68993.1}.
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DR EMBL; BFAA01003153; GCB68993.1; -; Genomic_DNA.
DR STRING; 75743.A0A401P7B4; -.
DR OMA; PSPMCRE; -.
DR Proteomes; UP000288216; Unassembled WGS sequence.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0004677; F:DNA-dependent protein kinase activity; IEA:InterPro.
DR GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; IEA:InterPro.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd05172; PIKKc_DNA-PK; 1.
DR Gene3D; 1.10.1070.11; Phosphatidylinositol 3-/4-kinase, catalytic domain; 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR037706; DNA-PK_dom.
DR InterPro; IPR046804; DNA-PKcs_N.
DR InterPro; IPR046803; DNAPKcs_CC1-2.
DR InterPro; IPR012582; DNAPKcs_CC3.
DR InterPro; IPR045581; DNAPKcs_CC5.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000403; PI3/4_kinase_cat_dom.
DR InterPro; IPR036940; PI3/4_kinase_cat_sf.
DR InterPro; IPR018936; PI3/4_kinase_CS.
DR InterPro; IPR003151; PIK-rel_kinase_FAT.
DR InterPro; IPR014009; PIK_FAT.
DR PANTHER; PTHR11139; ATAXIA TELANGIECTASIA MUTATED ATM -RELATED; 1.
DR PANTHER; PTHR11139:SF68; DNA-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT; 1.
DR Pfam; PF20500; DNA-PKcs_N; 1.
DR Pfam; PF20502; DNAPKcs_CC1-2; 1.
DR Pfam; PF08163; DNAPKcs_CC3; 1.
DR Pfam; PF19704; DNAPKcs_CC5; 1.
DR Pfam; PF02259; FAT; 1.
DR Pfam; PF00454; PI3_PI4_kinase; 1.
DR SMART; SM01344; NUC194; 1.
DR SMART; SM00146; PI3Kc; 1.
DR SUPFAM; SSF48371; ARM repeat; 3.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR PROSITE; PS51189; FAT; 1.
DR PROSITE; PS00915; PI3_4_KINASE_1; 1.
DR PROSITE; PS00916; PI3_4_KINASE_2; 1.
DR PROSITE; PS50290; PI3_4_KINASE_3; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW Kinase {ECO:0000256|ARBA:ARBA00022777};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000288216};
KW Serine/threonine-protein kinase {ECO:0000256|ARBA:ARBA00022527};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 2884..3476
FT /note="FAT"
FT /evidence="ECO:0000259|PROSITE:PS51189"
FT DOMAIN 3660..3891
FT /note="PI3K/PI4K catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50290"
SQ SEQUENCE 3891 AA; 443970 MW; A31A5543002BE8AC CRC64;
MAAGTQWRQG AGFEPERRLQ QLQQLLSASD SKNAALTSLT IVTNLSHDFL HSGQESRLAL
HSSLMFSKDE GLLIFLRKSL SNEDFRDCRD DVLKLITAFV EKIKETVGPY AVEIKDICLV
VYTKERAAKC KVAALDLLLV LLRCKISLQD FKIGEIFNKF YGELAQKSKL PDTVLEKVYE
LLGVLAEVQP TEMLDNSEKL FRAYLSELKT QMTSATREPK LLVVSGCLRG LIALLVNFTK
SVEEDPKTSK DIFDYAMKAI NLQIDIKRYA VPLAGLRLFT VHASQFSTCL MDSYQTLFEM
MSKWCGHSNA EMKKAGYTAL ESFLKEIALQ VAENAHLHKD KLQYFMQQFY DIIRTMESST
KELSIAIRGY GLFAAPCKAV NSQYVDFMYT ELIQRCKQMY LTEADTEDDN VYQLPSFLDS
LASVIFHIDK IPGVYTSVLE RLLVVQIDSF PQYSVRMQPV CCHSIVKLFI ALAGKGPVLW
NFISTVVHQG LIRVCSKPVL LPGEGMGKAE PWSNAQQSLD NGSVAAGKWR VPSYKDYLEL
FRSLLDCGKM KEFGLVETFQ MFDSPLKSLN QLLYDEFIKS VLKIIEKLDL TVDRQSQSEE
GGSGDINSSI VIPTSDPAAN LQPTKPKDFT ALFNLVEFCG ELLLEKHTDF FEQWVYRFGH
ELILRSTQFP LVSGFYKLLS VTMKIAQKIK YFEGVGAKTR KQCSKCPERF ACFALFGKFG
KEVAIRMKQY KDELLASCLT FVLSLPHDII ALDIKTFIPA LQTAFKLGLS HIPLADVGLD
ALEHWSIHIS KHAIEPYYKD ILPHLDGYLK TASNEIENNW EMMSINTSSK GGNKVLVRRL
KNARDLSMNE ESPAAAVRSR VVRLLGSLGG QINRSLVAAA TSEEMMKKCV AWVAERPLSF
AVPFTDMKPV IYLDSFLPHI SELALSANDR QTKVAACELL HSVVTYMLGK GSQISEGHQE
TMYKLNKHMF PVLLRLGCDV DKVTNQLFEP LVMQLIHWFT NNKKFESRDT VALLEAILDG
IVDPVDSTLR DFCGNCVQEF LKWSIKQTTP EQQQKSPVNT KSLFKRLYSL ALHPNTFKRL
GAALAFNSLY REFREENVLV EQFVFEALVV YLESLALAHS DDKSLGTVQQ CCDSIDHLKR
IIKQKARDLN TPVKRRVPRG FSSDQMVCLT DIVMWLLAQC GRPQTECRHK CMELFYELVP
LLPGNKTPSS WLDEIIKKNG VSFLVNKFEG AGNEHDKCSG IIFHPTFNDL QQPFSVRAAL
QWMDMLLAAL DCYNTFIGLH LIKPNELLGT YQKSGFMKAL HFFLKNLAMC DITAAEECFA
YGIKGILFSP REREEYNYSK CTIIVRIMEF VSMVLESCEQ DLWKVLEKDL LSSNLFELVA
IVVTDPPSIG FNMADVQVMK RLPDIGVRLL KAVLRLPYQD TLESNMRKQI TPQSIEGLCI
VDLYRQTDHD KLSSILLACK QLHKAGLLNS VLMCQTGSIG LPLNSKLLST VYKGIAPGDE
RKSLPSIDDS SKMLADGLLQ LSFSLGGQCE ELVSLLLNTV ELSVPLSGAS QRHFISFSHG
EYFYNLFPET INGELLRNLN SAVTQLMGST RENPKMVSIV LNGMLDQSFK DHTVRKTQGI
LLANAVLKNW AKLASWWSED SIPESKMAVL TLLSKVLQID SSVSYGDSHE AFSMVFQTYT
TILTDSTLAL NLKTKAVIIL PFFANLPEEN LRELKNALNQ FVAAHFPMKS DEFPKGTLRY
NNYIDCVKKL LDALELSQSP LLLKILTEVL CRDSKHVMEE AFQICFQNIV KRGTCEKQVV
LLEAAYEMFR NEEMLSDTTR QFVMDRSLLT LIVHCSPEAL REFFCKIVKD AMEIVQSRFI
KISESSFDNQ LTKKIGCYKL FEVMYGRLPK NDVYTDTSKI IQAFHSSGVI EGNKLSKTLL
KSCYDAVTEN MAGETQLVEK RRQYHCAAYN CMIAVLCCIF TEAKFYQGFL FSEKPEKNVF
ILENLIDVQR TYSFPVEIEV PVEKKRRYFA IRKEAREATD GDSDEPHYLS SQSYMVDSSL
SEEMSQFDFS TGVQSFSYSS QDPKVSVVRT RKTQEWTESN VMQNDIVELE MDEMNQHECM
ATMTALVNHM QKMNITPKVE EGQTPHEPPP WMKFLHGKLR NSATPLNIRL FIAKLIVNTE
EIFQPYAQQW LGPLLQFVVS GNNGGDGIHY MVVEIVVTVL SWTSVATPKG NPTNEILADR
LLAFLMKNSF HEKRAVFRHN LEIIKTVVEC WKDCLSIPYS LIFERFAGRD PNTKDNSVGI
QLLGIVLANN LPPYEPTCAI DSERYFQRLV QNISLTRYKE VYVAAAEVLG LVLQYLAENQ
MDDAIHDLVV KELQQLRNTK EDKFIVCLNK IVKHFPPFAD RFLNAVFFLL PKLYGVLKTQ
CLEVVMYRAE EIPDINIQLK SKDFAQIMSH RDEERQKVSL DIVYKIKAKL TSTELKEMLS
SVMAFISHPS PICRERLYDI LMWIYDNYRD PENQPDLESK EIYMSATDAL LQGLTDENPA
LQLYVRNFWS HENRLPTGTL DRIMAILGSL YCNKIETQYL SIATNVLLEM TSRSPDYCRE
MFEYPLSECK FQDYSIDSSW HQRSRILTPL FVETQATPGS IRIPTQDASA AGQRPVGGQI
RATQTHYEFT PTQNPGARNF NWLTGNSLDT FAEYSLLSSE SVSSSLLVYS KQSERQQRAA
MKPVGPEFGK KRLAVPGDEV DSKTKAVADQ RADILRLRRR FLKDREKESL IFARRNIREK
HLAKEKKTEQ KMKRDAQVTL YRCYRVGDLP DIQIKFSSLI APLQALAQKD HLLAKQLFTS
LFSGILEEVK KSKTAAEMSG IGKELMEHFN CLLKSTTLCF PPFVTCIQDM SRQHSDLLNL
DPSIVSSTAL TSLQQPMGIL LLELSLIHHT LPEEPPAKRA RGRNQLPPDT ARWVQLAKLY
RSLGDYDVLR GIFSEKIGTK SITQKALLAE GKSDYAEAVR IYDQALNSEE WGDGDPTDAE
KDFWEIAALE CYSHLTEWKS LEYCSTVNIG DKTPANIEEI WTDPFYQETY LPHMMRSKMK
LLQLGEVDQS LLTFIDNAMK MDQRKTVIES RYSQELSLLY ILQDDFDRAK YYVNNGIQIF
MQNYSSIDTL LHRSRLTKLQ SLQTLTEIQD FLSFITKGFN LTSDVPLRKL IRTWTGRYPE
SKLDPMSNNF SVSTKLLKEL HKESKDKNDW RMRWIQSYSR FSHKRCRSLD KPEQIVTVMK
TIPLLVEAEV GTLNTRQHAL RDQNTLLGTT YHIMANALKK DPSILQHIEE DRARTVIELS
GATSDDVVQV SIGLYRKAHE YLRNAVKKVE EELQSLSEEH VDTVGLIKAY MNLANFCDKC
LREEEESENF LNFPELQTFP ARVVENMLKA LKYNSREARL RFPRLLQIVE LYPAETQDLM
TRGVSSVPCW LFIGWISQMM AALDKEEASV IHHIIEDIAD SYPQAIVYPF MISSENYTFE
PSASGNRNKE FVERLKTKLN KGGVIENFIN SLEQLSNPDL LFKDWVNDFR NQLEKKVKIK
KILKNMYDEM YSNLASSQSP GIGAFRTRYA QTFKKLFDQY FGKEGSKLLD MNLTTFNEAT
KKIQDSINKK SCEQPGNLKE YSPWMSEFKP EYLRNELEIP GQYDGKSKPM PEYHARITGF
DERIKVMNSI RKPKRLIIRG NDEKEYPFLV KGGEDLRQDQ RIEQLFDIMN IILSRDAACS
QRGLQLKTYQ VIPMTSRLGL IEWLDNTCVV KDFLLSNVTE DERKRVPRRQ SPRELFTEWL
QKISGPKIEG PPQYGLMYMK ASRTETVKTF RNCEQAVPQD LLRRAFTKMS TTPEAFLTLR
SHFASSHALM CVSHWILGIG DRHLSNFMIN LETGGMVGID FGHAFGSATQ V
//
