ID A0A402A6S4_9CHLR Unreviewed; 809 AA.
AC A0A402A6S4;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 28-JUN-2023, entry version 10.
DE RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
GN ORFNames=KTT_47020 {ECO:0000313|EMBL:GCE14843.1};
OS Tengunoibacter tsumagoiensis.
OC Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
OC Dictyobacteraceae; Tengunoibacter.
OX NCBI_TaxID=2014871 {ECO:0000313|EMBL:GCE14843.1, ECO:0000313|Proteomes:UP000287352};
RN [1] {ECO:0000313|Proteomes:UP000287352}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Uno3 {ECO:0000313|Proteomes:UP000287352};
RA Wang C.M., Zheng Y., Sakai Y., Toyoda A., Minakuchi Y., Abe K., Yokota A.,
RA Yabe S.;
RT "Tengunoibacter tsumagoiensis gen. nov., sp. nov., Dictyobacter kobayashii
RT sp. nov., D. alpinus sp. nov., and D. joshuensis sp. nov. and description
RT of Dictyobacteraceae fam. nov. within the order Ktedonobacterales isolated
RT from Tengu-no-mugimeshi.";
RL Submitted (DEC-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|RuleBase:RU361166};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000256|PROSITE-ProRule:PRU10059, ECO:0000256|RuleBase:RU361166}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GCE14843.1}.
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DR EMBL; BIFR01000002; GCE14843.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A402A6S4; -.
DR OrthoDB; 9758662at2; -.
DR Proteomes; UP000287352; Unassembled WGS sequence.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030248; F:cellulose binding; IEA:InterPro.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR Gene3D; 2.60.40.290; -; 1.
DR Gene3D; 2.60.40.710; Endoglucanase-like; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001919; CBD2.
DR InterPro; IPR008965; CBM2/CBM3_carb-bd_dom_sf.
DR InterPro; IPR012291; CBM2_carb-bd_dom_sf.
DR InterPro; IPR001956; CBM3.
DR InterPro; IPR036966; CBM3_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR PANTHER; PTHR22298:SF29; ENDOGLUCANASE; 1.
DR Pfam; PF00553; CBM_2; 1.
DR Pfam; PF00942; CBM_3; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SMART; SM00637; CBD_II; 1.
DR SMART; SM01067; CBM_3; 1.
DR SUPFAM; SSF49384; Carbohydrate-binding domain; 2.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR PROSITE; PS51173; CBM2; 1.
DR PROSITE; PS51172; CBM3; 1.
DR PROSITE; PS00592; GH9_2; 1.
DR PROSITE; PS00698; GH9_3; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Cellulose degradation {ECO:0000256|RuleBase:RU361166};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Reference proteome {ECO:0000313|Proteomes:UP000287352}.
FT DOMAIN 502..655
FT /note="CBM3"
FT /evidence="ECO:0000259|PROSITE:PS51172"
FT DOMAIN 699..809
FT /note="CBM2"
FT /evidence="ECO:0000259|PROSITE:PS51173"
FT REGION 652..697
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 659..697
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 421
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10059"
FT ACT_SITE 460
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
FT ACT_SITE 469
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
SQ SEQUENCE 809 AA; 87539 MW; 67DD344F9B2D99EB CRC64;
MSLRSLFGTT WHRRVLSSLL ATGLMLSALA FSFAPSYLTH VKAAPAFNYG EALQKSIYFY
EEQSSGHKPD WNRVPWVGDS ALNDGSDVGL NLTGGWYDAG DHVKFGLPMA YSATMLAWGV
IANRDAYTSD GQLTNIMNNL HFVNDYFIKA HPAPNVLYGQ VGNGSDDHAF WGPAEVMQMN
RPAYKIDASC PGSDLAGETA AAMAASSIVF SSTDATYAST LLDHAKQLYS FADNYRGKYD
SCITAASGYY TSYSGYNDEL VWGAIWLYQA TNDPSYLTKA ENYYANLGTE PQSTTHSYKW
TIGWDDVSYG TYVLLAQITG QQQYKDDAQR WLDYWTVGVN GQKITYSPGG EAFLDQWGSL
RYAADTAFVA LIYADYLGAG NALYSRYHDF AVGQINYILG ANPRNCSYMV GFGSCYPQTP
HHRESHDSWT NNIGNPTYQR HILYGALVGG PSSANDSFTD NRQDYATNEP ADDYNAALTG
ALARLYKEYG GSPVASMPDK AKDDDEIYSE AAINASGSNF TEIKAEFINK SGWPARITSN
LSLRYYFTLD AGVTPNMLTL NTNYSECGSN FLSGPTQYAG NIYYVTASCA GTPIYPGGQS
AFQKQVQFRI TSSGAWDPSN DWSFQGVAPN GSNPVKVTNL PVFDGNTQVW GQLPDGSGLP
TPTPTPVTTP TPTPVTTPTP TPVTTPTPTP TATPTPTPTP ITGSACSVSY VIQNQWPGGF
TANMTITNTG SSTINGWTLA FTFPNGQQIT QIWNGALNQQ GSQVAIQNLS YNNVIAPGGT
VNPGFNGSWT TSNSAPTSFT LNGTTCSMS
//
