ID A0A409WK05_PSICY Unreviewed; 1748 AA.
AC A0A409WK05;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 27-MAR-2024, entry version 16.
DE RecName: Full=RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00012494};
DE EC=2.7.7.48 {ECO:0000256|ARBA:ARBA00012494};
GN ORFNames=CVT25_010631 {ECO:0000313|EMBL:PPQ78845.1};
OS Psilocybe cyanescens.
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Agaricomycetidae; Agaricales; Agaricineae; Strophariaceae; Psilocybe.
OX NCBI_TaxID=93625 {ECO:0000313|EMBL:PPQ78845.1, ECO:0000313|Proteomes:UP000283269};
RN [1] {ECO:0000313|EMBL:PPQ78845.1, ECO:0000313|Proteomes:UP000283269}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=2631 {ECO:0000313|EMBL:PPQ78845.1,
RC ECO:0000313|Proteomes:UP000283269};
RX PubMed=30283667; DOI=10.1002/evl3.42;
RA Reynolds H.T., Vijayakumar V., Gluck-Thaler E., Korotkin H.B.,
RA Matheny P.B., Slot J.C.;
RT "Horizontal gene cluster transfer increased hallucinogenic mushroom
RT diversity.";
RL Evol. Lett. 2:88-101(2018).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.48; Evidence={ECO:0000256|ARBA:ARBA00024517};
CC -!- SIMILARITY: Belongs to the RdRP family.
CC {ECO:0000256|ARBA:ARBA00005762}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PPQ78845.1}.
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DR EMBL; NHYD01003404; PPQ78845.1; -; Genomic_DNA.
DR STRING; 93625.A0A409WK05; -.
DR InParanoid; A0A409WK05; -.
DR OrthoDB; 2905532at2759; -.
DR Proteomes; UP000283269; Unassembled WGS sequence.
DR GO; GO:0004386; F:helicase activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0031047; P:regulatory ncRNA-mediated gene silencing; IEA:UniProt.
DR CDD; cd18808; SF1_C_Upf1; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR041679; DNA2/NAM7-like_C.
DR InterPro; IPR041677; DNA2/NAM7_AAA_11.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR007855; RNA-dep_RNA_pol_euk-typ.
DR InterPro; IPR047187; SF1_C_Upf1.
DR PANTHER; PTHR23079; RNA-DEPENDENT RNA POLYMERASE; 1.
DR PANTHER; PTHR23079:SF55; RNA-DIRECTED RNA POLYMERASE; 1.
DR Pfam; PF13086; AAA_11; 2.
DR Pfam; PF13087; AAA_12; 1.
DR Pfam; PF05183; RdRP; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022484};
KW Reference proteome {ECO:0000313|Proteomes:UP000283269};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884};
KW RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 1311..1446
FT /note="DNA2/NAM7 helicase helicase"
FT /evidence="ECO:0000259|Pfam:PF13086"
FT DOMAIN 1449..1516
FT /note="DNA2/NAM7 helicase helicase"
FT /evidence="ECO:0000259|Pfam:PF13086"
FT DOMAIN 1524..1726
FT /note="DNA2/NAM7 helicase-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF13087"
SQ SEQUENCE 1748 AA; 197897 MW; 77F9782CBD6E701D CRC64;
MPPPVLLREN PAPDDEKWEF TYNPASLSTR QKPGVKYGKL VTLRPNGIFL QIEQFPSNRI
LNSDDGGPSK FVLLSIDRLF RFQEQPMKVA VDYVLRLFKR GLWLNGVQYR FYGHSNSQLR
GRSCFLREAN SDQELDDRIY RMGDFKRIMN VAKRAKRIGL MFSSAEIDYS LDPRRIGDIP
DITDGDEVFS DGCGLVSKWL AIQLSKKKKI IFRGVRYTPT VFQIRYLGYK GVLMLHPDLD
KNGLQHVAQF RKSMKKFATT VDHTFSVVGH SKPYSFGRLN NDIIVLLSSL GVSSSAFLQK
QQEYFDWIMS ASGSSDNEKD PVSAVDFLFC LSEYTLAERA LLEGVDSEPV KREIRRLQNS
EVAGARQEGT NKFKSRIIVH KSRRLYGVCD PYQVLEEGQV HIRISVGRKG ATTPIHGDVI
IVRNPCLHPG DILKLRAVDH PKLSHLLDCL VFASVARPGH KAAPSMSSGG DLDGDEFFVC
WDKDIIPTKI AEVKFSTFLS HPSQVDEYSY DYPGNREFTK KDITRTDLGV HFASYTSFGI
RVVILIVLHR SGVAKVSALH AKWARSSPKG ALCVECQELN ALHSQSVDGA NIRIPEKLST
PPAPPDDVPY ILDLLEKAAE KFSTQFTESA SRRAEIATST SREDAEQLLG RLLQSQQNSL
SEFELFNLAY RLSQKHTIDI RPYLAQLDFS ALTTGEKHII STTLSLSDVE YPELWNSLVR
SDILTPQDLY QRNLAQPFQL HRLYSSRTSG LTTFFEYLRR ATQEYTRKML IIQTDPRFAI
GIFMRGKIPW DEDLEVKESN KDGDAMNVVV CSFMPSTAST IATLRPCPTG YRLHCSDYRF
QLYERHIGNT FIYVNRPPSE LKAEVSTSIA LDKISRTVQR QLGRVNKAPA LNIELHVVSN
RDRVAHQLFD LWFEHVPTEE YERRFQRNPA PYRLNDLKDV DWDSDPTKSI KDVFFRDIPG
VPLSSNRLKH DKSSMAERLS RRMMPEIDKI MEFAIKYHAE DELFLVFGNV ITRQPLSLDF
VKEWIERCPI LTFTLLKVYP PNDHDDLLPE LQSISHLIIR NIIRSANETK ISALVALEKL
AKTIGSLPLQ HYIDLLMLAA QSVRAKTLVQ EVLLVLNERR LEYCLPSPKN YYGHKHALGV
AFDRAEEAAE ECPCNEDGRP RQKMRVPPSH AKLQFGPDHL ENAEVTATIR IDAKTGVRLH
SHVRLQAASR AENRWIDAPI MDGLVVQALK GQLKIKLLHP APPEMEVMDW NLYDAGSTAT
SNAMMDALQR LLHDESECCL FYSLITGSGP VISDDAVFKD FDRSQISPES QLNESQIKAV
ESWRNPLSLI WGPPGTGKTT VMVQILRDII KSYKETPKIL MTASTHNGTA VYLNDPILRV
LNSSALAVDN VLERFIAIND VENLLREEQI LRVATDQSKV NPSLHHYTVN DRVGGDMNEN
NKLFKQAQDR VEKAVLIFST CAGAGLGILR KPDFDIALID EASQITEPCA LIPLVKGIKR
AVIVGDHVQL RPTVKNMGKT LESDVSLLER LYTKRDEVPD GLAKTMLDVQ YRSPRILNIF
PSEEFYDGQL KTHEANAEVS NFLSLSQFPW PVKDRTVIPT VFIQCSEEED MGDRSKSNQG
QVEVIHRLLP LISSQRPDTP AEPKLEGLKT TVLSPYTKQI QALRRKLPSS VTCSTIDSFQ
GRESDVIIFS TVRCNVEGDV GFLDDPRRLN VMWTRARLAL IIVGDRRTMS SNPLWKRALD
ACTECSLT
//
