ID A0A411WFT2_9GAMM Unreviewed; 718 AA.
AC A0A411WFT2;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE SubName: Full=Polysaccharide biosynthesis tyrosine autokinase {ECO:0000313|EMBL:QBH95171.1};
DE EC=2.7.10.2 {ECO:0000313|EMBL:QBH95171.1};
GN ORFNames=EKN56_01350 {ECO:0000313|EMBL:QBH95171.1};
OS Limnobaculum zhutongyuii.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Budviciaceae; Limnobaculum.
OX NCBI_TaxID=2498113 {ECO:0000313|EMBL:QBH95171.1, ECO:0000313|Proteomes:UP000293154};
RN [1] {ECO:0000313|EMBL:QBH95171.1, ECO:0000313|Proteomes:UP000293154}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CF-458 {ECO:0000313|EMBL:QBH95171.1,
RC ECO:0000313|Proteomes:UP000293154};
RA Ge Y.;
RT "Pragia sp. nov. isolated from the gut tract of Carduelis flavirostris.";
RL Submitted (MAR-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl-
CC [protein]; Xref=Rhea:RHEA:10596, Rhea:RHEA-COMP:10136, Rhea:RHEA-
CC COMP:10137, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:46858,
CC ChEBI:CHEBI:82620, ChEBI:CHEBI:456216;
CC Evidence={ECO:0000256|ARBA:ARBA00001074};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000256|ARBA:ARBA00004429}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004429}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the etk/wzc family.
CC {ECO:0000256|ARBA:ARBA00008883}.
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DR EMBL; CP034752; QBH95171.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A411WFT2; -.
DR KEGG; prag:EKN56_01350; -.
DR OrthoDB; 9775724at2; -.
DR Proteomes; UP000293154; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004715; F:non-membrane spanning protein tyrosine kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0045226; P:extracellular polysaccharide biosynthetic process; IEA:InterPro.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd05387; BY-kinase; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR025669; AAA_dom.
DR InterPro; IPR032807; GNVR.
DR InterPro; IPR003856; LPS_length_determ_N_term.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005702; Wzc-like_C.
DR NCBIfam; TIGR01007; eps_fam; 1.
DR PANTHER; PTHR32309; TYROSINE-PROTEIN KINASE; 1.
DR PANTHER; PTHR32309:SF13; TYROSINE-PROTEIN KINASE ETK-RELATED; 1.
DR Pfam; PF13614; AAA_31; 1.
DR Pfam; PF13807; GNVR; 1.
DR Pfam; PF02706; Wzz; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Cell inner membrane {ECO:0000256|ARBA:ARBA00022519};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Kinase {ECO:0000256|ARBA:ARBA00022777, ECO:0000313|EMBL:QBH95171.1};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:QBH95171.1};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius};
KW Tyrosine-protein kinase {ECO:0000256|ARBA:ARBA00023137}.
FT TRANSMEM 32..51
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 426..445
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 16..108
FT /note="Polysaccharide chain length determinant N-terminal"
FT /evidence="ECO:0000259|Pfam:PF02706"
FT DOMAIN 367..447
FT /note="Tyrosine kinase G-rich"
FT /evidence="ECO:0000259|Pfam:PF13807"
FT DOMAIN 525..648
FT /note="AAA"
FT /evidence="ECO:0000259|Pfam:PF13614"
FT COILED 255..289
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 718 AA; 80610 MW; C870D6601E379398 CRC64;
MSEIRSASYK NSKEEDEINL GKILGTIIDK KWLLVSITII LTLIGAFYAL FTTPIYRADV
LIQIEKNSTN NLLNNVSEIL SNNIMPSTAA EIELIKSRMV IGKTVSDLGL NIEVSEKYFP
IIGEKLSNLL GIKPGQVAIS KFDIPETMYN YDFILTVLPD NQYSLKGNDI DIIGKIALIE
KSNNISILIS DIIAPVGTIF KIKKKDYLTT INNVLSDLSI QDTTKDGGIL RLYVTGQDKD
KIRKIADSIS NNYLQQNVER KSEEAANSLA FLREQLPLIK RQLEEAEIKL NTFRQTNESV
DLTLEAKSIL DSMVSITSQL NEVTFKEAEI SKLYTKEHPA YRSLLEKKNT LIQEKERLNK
SVTNMPKTQQ EILRLTRDVQ SGQEIYMLLL NKQQELSINK ASTVGNARII DKAITQPNKI
KPKSSLIILL SFIAGVFLSI LIIFIQELFR RTINDSSQLE SIGINVYANI PLSKNLKNRN
RRLIKQPLEN HTKLLALEQE TDLAVEAIRS LRTSLHFAML DASNRILMIT GATSGIGKSF
ISSNLAVVVA QSGVRVLLID ADMRKGGLHK LFKITMGNGL SDILSNNKEI EKCVYKTPVD
GLDFIPRGSI PPNPSELLMN KYFSSFIEWA GANYDLIIFD TPPILATTDS MIIGRYSGTN
LLVARFEHSS VREVEMSLKR FEQNGLTIKG LVLNAVQQRA SKFYGDGVFE YYSYQSEK
//
