ID A0A416R6Z7_9FIRM Unreviewed; 404 AA.
AC A0A416R6Z7;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 27-MAR-2024, entry version 16.
DE RecName: Full=Pyridinium-3,5-bisthiocarboxylic acid mononucleotide nickel insertion protein {ECO:0000256|HAMAP-Rule:MF_01074};
DE Short=P2TMN nickel insertion protein {ECO:0000256|HAMAP-Rule:MF_01074};
DE EC=4.99.1.12 {ECO:0000256|HAMAP-Rule:MF_01074};
DE AltName: Full=Nickel-pincer cofactor biosynthesis protein LarC {ECO:0000256|HAMAP-Rule:MF_01074};
GN Name=larC {ECO:0000256|HAMAP-Rule:MF_01074,
GN ECO:0000313|EMBL:RHR08235.1};
GN ORFNames=DWX58_10490 {ECO:0000313|EMBL:RHR08235.1};
OS Pseudoflavonifractor sp. AF19-9AC.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae;
OC Pseudoflavonifractor.
OX NCBI_TaxID=2292244 {ECO:0000313|EMBL:RHR08235.1, ECO:0000313|Proteomes:UP000285063};
RN [1] {ECO:0000313|EMBL:RHR08235.1, ECO:0000313|Proteomes:UP000285063}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AF19-9AC {ECO:0000313|EMBL:RHR08235.1,
RC ECO:0000313|Proteomes:UP000285063};
RA Zou Y., Xue W., Luo G.;
RT "A genome reference for cultivated species of the human gut microbiota.";
RL Submitted (AUG-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in the biosynthesis of a nickel-pincer cofactor
CC ((SCS)Ni(II) pincer complex). Binds Ni(2+), and functions in nickel
CC delivery to pyridinium-3,5-bisthiocarboxylic acid mononucleotide
CC (P2TMN), to form the mature cofactor. Is thus probably required for the
CC activation of nickel-pincer cofactor-dependent enzymes.
CC {ECO:0000256|HAMAP-Rule:MF_01074}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Ni(II)-pyridinium-3,5-bisthiocarboxylate mononucleotide =
CC Ni(2+) + pyridinium-3,5-bisthiocarboxylate mononucleotide;
CC Xref=Rhea:RHEA:54784, ChEBI:CHEBI:49786, ChEBI:CHEBI:137372,
CC ChEBI:CHEBI:137373; EC=4.99.1.12; Evidence={ECO:0000256|HAMAP-
CC Rule:MF_01074};
CC -!- SIMILARITY: Belongs to the LarC family. {ECO:0000256|HAMAP-
CC Rule:MF_01074}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RHR08235.1}.
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DR EMBL; QUGI01000005; RHR08235.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A416R6Z7; -.
DR Proteomes; UP000285063; Unassembled WGS sequence.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR GO; GO:0051604; P:protein maturation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.70.1380; Transcriptional regulatory protein pf0864 domain like; 1.
DR HAMAP; MF_01074; LarC; 1.
DR InterPro; IPR002822; Ni_insertion.
DR NCBIfam; TIGR00299; nickel pincer cofactor biosynthesis protein LarC; 1.
DR PANTHER; PTHR36566; NICKEL INSERTION PROTEIN-RELATED; 1.
DR PANTHER; PTHR36566:SF1; NICKEL INSERTION PROTEIN-RELATED; 1.
DR Pfam; PF01969; Ni_insertion; 1.
PE 3: Inferred from homology;
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_01074};
KW Nickel {ECO:0000256|ARBA:ARBA00022596, ECO:0000256|HAMAP-Rule:MF_01074};
KW Reference proteome {ECO:0000313|Proteomes:UP000285063}.
SQ SEQUENCE 404 AA; 44120 MW; E30FB14EB3BCD954 CRC64;
MKTLYLDCGM GAAGDMLAAA LLELIPDRDG FIGRMNSFGL PGVTVTQEVS VKCGITGTHF
SVKVSGEEEG SHDYHNCGNH DPEHGHGYHP HRGMDEIAHI VCECLPVPQK VKKDVMAVYK
LIAEAESHAH GVPVSDIHFH EVGTMDAIAD ITVVCLLMNE IAPDEVVVSP VHVGSGKVRC
AHGVLPVPAP ATAYILKDVP IYGGQIQGEL CTPTGAALLK YFATKFGAMP VMRTQAIGYG
MGKKDFETAN CVRAMLGEGE GKTDAVLELS CNVDDMTAEQ IGFAMERLFD GGALDVYTIP
IGMKKSRPGT LIRVMCRERD KERLVPLIFK YTTTIGVRET KMQRYILDRD VTTLETPYGT
IRRKDSSGYG VRRSKYEYED LARIAREHGI RMEDVVAGIE DQSS
//
