ID A0A417XZ76_9ACTN Unreviewed; 459 AA.
AC A0A417XZ76;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 24-JAN-2024, entry version 16.
DE RecName: Full=Diacylglycerol O-acyltransferase {ECO:0000256|ARBA:ARBA00013244, ECO:0000256|RuleBase:RU361241};
DE EC=2.3.1.20 {ECO:0000256|ARBA:ARBA00013244, ECO:0000256|RuleBase:RU361241};
GN ORFNames=D0Z08_18010 {ECO:0000313|EMBL:RHW25672.1};
OS Nocardioides immobilis.
OC Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
OC Nocardioidaceae; Nocardioides.
OX NCBI_TaxID=2049295 {ECO:0000313|EMBL:RHW25672.1, ECO:0000313|Proteomes:UP000283644};
RN [1] {ECO:0000313|EMBL:RHW25672.1, ECO:0000313|Proteomes:UP000283644}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCTCC AB 2017083 {ECO:0000313|EMBL:RHW25672.1,
RC ECO:0000313|Proteomes:UP000283644};
RA Li C., Wang G.;
RT "Genome sequencing of Nocardioides immobilis CCTCC AB 2017083 for
RT comparison to Nocardioides silvaticus.";
RL Submitted (SEP-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC Evidence={ECO:0000256|ARBA:ARBA00001039,
CC ECO:0000256|RuleBase:RU361241};
CC -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004771, ECO:0000256|RuleBase:RU361241}.
CC -!- PATHWAY: Lipid metabolism. {ECO:0000256|ARBA:ARBA00005189}.
CC -!- SIMILARITY: Belongs to the long-chain O-acyltransferase family.
CC {ECO:0000256|ARBA:ARBA00009587, ECO:0000256|RuleBase:RU361241}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RHW25672.1}.
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DR EMBL; QXGH01000022; RHW25672.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A417XZ76; -.
DR OrthoDB; 9810950at2; -.
DR UniPathway; UPA00282; -.
DR Proteomes; UP000283644; Unassembled WGS sequence.
DR GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0019432; P:triglyceride biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR014292; Acyl_transf_WS/DGAT.
DR InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR NCBIfam; TIGR02946; acyl_WS_DGAT; 1.
DR PANTHER; PTHR31650:SF1; DIACYGLYCEROL O-ACYLTRANSFERASE TGS4-RELATED; 1.
DR PANTHER; PTHR31650; O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEIN; 1.
DR Pfam; PF06974; WS_DGAT_C; 1.
DR Pfam; PF03007; WS_DGAT_cat; 1.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 2.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315,
KW ECO:0000256|RuleBase:RU361241};
KW Glycerol metabolism {ECO:0000256|ARBA:ARBA00022798,
KW ECO:0000256|RuleBase:RU361241};
KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00022516,
KW ECO:0000256|RuleBase:RU361241};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098,
KW ECO:0000256|RuleBase:RU361241};
KW Reference proteome {ECO:0000313|Proteomes:UP000283644};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU361241}.
FT DOMAIN 17..264
FT /note="O-acyltransferase WSD1-like N-terminal"
FT /evidence="ECO:0000259|Pfam:PF03007"
FT DOMAIN 308..453
FT /note="O-acyltransferase WSD1 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF06974"
SQ SEQUENCE 459 AA; 49743 MW; B6D3E9AF8D941A09 CRC64;
MGRGTGRTAM NNIAMGDAMF LLGEAPSRPA QVITLQLYRS PDGCDADEWV ADYFQHLLSH
EDLKPVFRRR PARTALSPST LRWVEDEPVE IDHHVRHSAL PGQGRVRELL EAVAVQHGVP
LDRRRPLWEF HVFEGLEGGR FATAFKTHHA IADGMSLAKH VLGSLTEDPE VRGCIPPWAA
RPQAAERPTQ QSPSISLAPV GRSVSVVRSL VHMSRDANSR VPFEAPPSPL NVKVGGARRF
AGDQWPVERL KAVASAASCT VNDVGLAMTG GALRAYLDEL GLLPDRSLIA MVPVSVRRPG
EAIADGEGNA FGAMLCDLAT AVDDPAARLG SIADQTRTAK QRFSSMSVAE VLAVSKLIMG
GAVLSSFTGI TALPRHPFNL IISNVPATNR PLYYNGARMT DIYPVSMISE AQAMNVTLTR
YDDLMTFGIV GDRRALPHLQ RMLVHLDEAL TALEKAVLG
//
