ID A0A417YZD9_9BACI Unreviewed; 750 AA.
AC A0A417YZD9;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=D1B31_00790 {ECO:0000313|EMBL:RHW43247.1};
OS Neobacillus notoginsengisoli.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Neobacillus.
OX NCBI_TaxID=1578198 {ECO:0000313|EMBL:RHW43247.1, ECO:0000313|Proteomes:UP000284416};
RN [1] {ECO:0000313|EMBL:RHW43247.1, ECO:0000313|Proteomes:UP000284416}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 30743 {ECO:0000313|EMBL:RHW43247.1,
RC ECO:0000313|Proteomes:UP000284416};
RX PubMed=28786779; DOI=10.1099/ijsem.0.001975;
RA Zhang M.Y., Cheng J., Cai Y., Zhang T.Y., Wu Y.Y., Manikprabhu D., Li W.J.,
RA Zhang Y.X.;
RT "Bacillus notoginsengisoli sp. nov., a novel bacterium isolated from the
RT rhizosphere of Panax notoginseng.";
RL Int. J. Syst. Evol. Microbiol. 67:2581-2585(2017).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RHW43247.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; QWEG01000001; RHW43247.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A417YZD9; -.
DR OrthoDB; 9810135at2; -.
DR Proteomes; UP000284416; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 127..406
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 407..674
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 148..155
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 750 AA; 84751 MW; 8AFFAD0CD0184B8E CRC64;
MKSAIYQNGT VHLELLGRDR FQKIYMAGRN GELSCPSCLE PVRLYLGIEK DPHFFHLYHP
EKICLEASET IKETRQIEGF RVPSAKPVGE GFLGAQPLAI GAATATASLP ILDNPHDYVK
LLEAEGFKLD GNQAAAVTET EGPILVLAGA GSGKTRVLTA RTAYMLDVRG IKPSEIMLVT
FTTKAAQEMK SRLLRYPNMK SGLLSKLVSG TFHSIFYRIL MHADGAKWNN NRLLKKEWQR
EKIVKEAGRE LGLSEKEFPY DLALQQIGFW KNSLLAPAKI KPDSEWEETV LMLYERYERY
KQDQGLFDFD DMLTGCYELF QTAPELLAMY QERFRYFLID EFQDINKVQY ELIKMLSAKT
KNVCVVGDDD QTIYSFRGSS PEFLLNFEHD FPGARLILLS QNYRSSNEIV AAANRIISYN
KKRRRKEMAA NISEGLPPVA FFPYDEEEEA TMIVTDIQEK IAGGADPSDF AILYRTHAGS
RAVFERLASS NLPFRIEQDA ESFYERRTVR TVLGFLKLAI NEDDIDAVAA ILPALFLKQD
VLSDIKASTI LDDCSALEAL GKVKTAHAFQ MKKLKTAVTA IKSMRHLAPY AAIERIEKEL
GFLDYLKKRG SEGNKLEKGS DDVKDLKVAA KNFDSSQTLL EHASHMAAMN REIKNLSKHF
TSSITLSTIH RAKGLEYKTV YILGAVDGSL PHDYALESLR NGDTAPLEEE RRLFYVAITR
AMERLYISVP SMRRNRAANP TRFLTPVRKD
//
