ID A0A418NW25_9SPHN Unreviewed; 719 AA.
AC A0A418NW25;
DT 08-MAY-2019, integrated into UniProtKB/TrEMBL.
DT 08-MAY-2019, sequence version 1.
DT 27-MAR-2024, entry version 15.
DE RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000256|HAMAP-Rule:MF_00255};
DE EC=6.1.1.14 {ECO:0000256|HAMAP-Rule:MF_00255};
DE AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000256|HAMAP-Rule:MF_00255};
DE Short=GlyRS {ECO:0000256|HAMAP-Rule:MF_00255};
GN Name=glyS {ECO:0000256|HAMAP-Rule:MF_00255};
GN ORFNames=D2V07_00665 {ECO:0000313|EMBL:RIV88824.1};
OS Aurantiacibacter zhengii.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC Erythrobacteraceae; Aurantiacibacter.
OX NCBI_TaxID=2307003 {ECO:0000313|EMBL:RIV88824.1, ECO:0000313|Proteomes:UP000286576};
RN [1] {ECO:0000313|EMBL:RIV88824.1, ECO:0000313|Proteomes:UP000286576}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=V18 {ECO:0000313|EMBL:RIV88824.1,
RC ECO:0000313|Proteomes:UP000286576};
RA Fang C., Wu Y.-H., Sun C., Wang H., Cheng H., Meng F.-X., Wang C.-S.,
RA Xu X.-W.;
RT "Erythrobacter zhengii sp.nov., a bacterium isolated from deep-sea
RT sediment.";
RL Submitted (AUG-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC EC=6.1.1.14; Evidence={ECO:0000256|ARBA:ARBA00000201,
CC ECO:0000256|HAMAP-Rule:MF_00255};
CC -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC {ECO:0000256|ARBA:ARBA00011209, ECO:0000256|HAMAP-Rule:MF_00255}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496,
CC ECO:0000256|HAMAP-Rule:MF_00255}.
CC -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00008226, ECO:0000256|HAMAP-Rule:MF_00255}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RIV88824.1}.
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DR EMBL; QXFL01000001; RIV88824.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A418NW25; -.
DR OrthoDB; 9775440at2; -.
DR Proteomes; UP000286576; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR InterPro; IPR008909; DALR_anticod-bd.
DR InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR NCBIfam; TIGR00211; glyS; 1.
DR PANTHER; PTHR30075:SF2; GLYCINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL 2; 1.
DR PANTHER; PTHR30075; GLYCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF05746; DALR_1; 1.
DR Pfam; PF02092; tRNA_synt_2f; 1.
DR PRINTS; PR01045; TRNASYNTHGB.
DR SUPFAM; SSF109604; HD-domain/PDEase-like; 1.
DR PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|HAMAP-Rule:MF_00255};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00255}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00255};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|HAMAP-Rule:MF_00255};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00255};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917, ECO:0000256|HAMAP-
KW Rule:MF_00255}; Reference proteome {ECO:0000313|Proteomes:UP000286576}.
FT DOMAIN 631..711
FT /note="DALR anticodon binding"
FT /evidence="ECO:0000259|Pfam:PF05746"
SQ SEQUENCE 719 AA; 79075 MW; AA35324CBCD600FF CRC64;
MSDFLLELRS EEIPARMQAG ARAELEKLFR REMEAAGVDF GDITIWSTPR RLALITRGLP
DATQPVSSEE KGPPVGAPDQ AVDGFCRKQG VSRDDLEVRD VKGRETYFAV KNIPGRATKD
LLAEAIPAII RDFSWPKSMR WGEASLSTES LRWVRPLSGI VALLGEELVE CEVHGVTSGW
VTLGHRFHHS GDITIGSADD YAEKLRACHV IVDHEERQDI VRSAAAKVAA DAGLKLVEDE
GLVIENAGLT EWPVPLLGRF DEDFLDVPPE VIQLTARVNQ KYFVCEDSAG KLANAFVCTA
NIEAADGGAA IVDGNRKVLA ARLSDARFFW ELDQKTPLRE HAKKLERITF HEKLGTVADK
VDRVAKLAAW LTSDARAPNG DHNMARQAAE LSKSDLVTEM VGEFPELQGL MGGYYAEKEG
LPQEVADAIR DHYKPVGQDD EVPTAPLTVA VSLADKLDNL FSFFNIDILP TGSKDPFALR
RAALGYLRLV QANGLELSLE QAAQAWGGLG QAKLGTRVAE FLSDRLAVQM REEGVRHDYI
AASREWQGHT DARTDRIEAR ARALAEFLST DNGTNLLAGY KRAANILKKE DWRGIEGEIA
RTGEEDPLAL VDDPDMKAVI DAKMSERHAR ELSYTPEPAE KALIEALDAA EPRASQAIEK
EDFATAMSAL ASLRAPIDAF FEEVTVNDPD EDKRASRLDL LARFRAAVHN VADFSKIEG
//
